ZmNAC55, a maize stress-responsive NAC transcription factor, confers drought resistance in transgenic Arabidopsis

Mao, Hude; Yu, Lijuan; Han, Ran; Li, Zhanjie; Liu, Hui

doi:10.1016/j.plaphy.2016.04.018

Cited by 96 publications

(72 citation statements)

References 46 publications

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“…In addition, the phylogenetic tree of common bean NAC genes and known-function NAC genes from other species also suggested that these 22 NAC genes may be related to drought stress. For example, one group included five common bean NAC genes and 14 known-function NAC genes that are all induced by drought stress [1, 39, 40, 42, 44, 65–70]. The members of this subfamily are also the most widely studied and play important roles in the NAC family.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis and discovery of drought-related NAC transcription factors in common bean

Wang

2016

BMC Plant Biol

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BackgroundCommon bean (Phaseolus vulgaris L.) is an important warm-season food legume. Drought is the most important environmental stress factor affecting large areas of common bean via plant death or reduced global production. The NAM, ATAF1/2 and CUC2 (NAC) domain protein family are classic transcription factors (TFs) involved in a variety of abiotic stresses, particularly drought stress. However, the NAC TFs in common bean have not been characterized.ResultsIn the present study, 86 putative NAC TF proteins were identified from the common bean genome database and located on 11 common bean chromosomes. The proteins were phylogenetically clustered into 8 distinct subfamilies. The gene structure and motif composition of common bean NACs were similar in each subfamily. These results suggest that NACs in the same subfamily may possess conserved functions. The expression patterns of common bean NAC genes were also characterized. The majority of NACs exhibited specific temporal and spatial expression patterns. We identified 22 drought-related NAC TFs based on transcriptome data for drought-tolerant and drought-sensitive genotypes. Quantitative real-time PCR (qRT-PCR) was performed to confirm the expression patterns of the 20 drought-related NAC genes.ConclusionsBased on the common bean genome sequence, we analyzed the structural characteristics, genome distribution, and expression profiles of NAC gene family members and analyzed drought-responsive NAC genes. Our results provide useful information for the functional characterization of common bean NAC genes and rich resources and opportunities for understanding common bean drought stress tolerance mechanisms.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0882-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Comprehensive analysis and discovery of drought-related NAC transcription factors in common bean

Wang

2016

BMC Plant Biol

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“…In a previous study, improved drought tolerance associated with reduced water transpiration rates of transgenic Arabidopsis plants ectopically expressing chickpea (Cicer arietinum) CarNAC4 has also been reported [73]. Furthermore, transgenic Arabidopsis harboring the maize ZmNAC55 showed better drought tolerance, which was partially attributed to the faster stomatal closure rates, leading to a slower cellular dehydration process under water-deficit conditions [74]. In another research, the enhanced tolerance of TaNAC2-transgenic Arabidopsis lines to drought was also featured with a lower water loss rate in detached leaves coupled with lower osmotic potential [75].…”

Section: Gmnac019-transgenic Plants Displayed Better Drought Tolerancmentioning

confidence: 87%

“…Functional analyses of TaNAC29 [50], ZmSNAC1 [51], AhNAC2 [91] and OsNAC52 [92] in Arabidopsis also revealed that these TFs act as positive regulators for plant responses to dehydration or drought in the ABA-dependent signaling pathway. It is suggested that this ABA-responsive feature of the examined NACs may enhance the stomatal closure, and thus, reduce water transpiration from leaves [73,74]. On the other hand, the foxtail millet (Setaria italica) SiNAC110 has been demonstrated to exhibit ABA-independent actions in enhancing the drought tolerance of SiNAC110-transgenic Arabidopsis plants [93].…”

Section: Gmnac019-transgenic Plants Were More Sensitive To Abamentioning

confidence: 99%

The Soybean GmNAC019 Transcription Factor Mediates Drought Tolerance in Arabidopsis in an Abscisic Acid-Dependent Manner

Hoang

Nguyen

et al. 2019

IJMS

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Being master regulators of gene expression, transcription factors (TFs) play important roles in determining plant growth, development and reproduction. To date, many TFs have been shown to positively mediate plant responses to environmental stresses. In the current study, the biological functions of a stress-responsive NAC [NAM (No Apical Meristem), ATAF1/2 (Arabidopsis Transcription Activation Factor1/2), CUC2 (Cup-shaped Cotyledon2)]-TF encoding gene isolated from soybean (GmNAC019) in relation to plant drought tolerance and abscisic acid (ABA) responses were investigated. By using a heterologous transgenic system, we revealed that transgenic Arabidopsis plants constitutively expressing the GmNAC019 gene exhibited higher survival rates in a soil-drying assay, which was associated with lower water loss rate in detached leaves, lower cellular hydrogen peroxide content and stronger antioxidant defense under water-stressed conditions. Additionally, the exogenous treatment of transgenic plants with ABA showed their hypersensitivity to this phytohormone, exhibiting lower rates of seed germination and green cotyledons. Taken together, these findings demonstrated that GmNAC019 functions as a positive regulator of ABA-mediated plant response to drought, and thus, it has potential utility for improving plant tolerance through molecular biotechnology.

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“…Similar effects were observed in other plant species, including tomato (Lee et al, 2003), sugarcane (Reis et al, 2014), and Lolium perenne (Li et al, 2011). Transcriptome analysis has been used to elucidate the gene regulatory networks involved in abiotic stress responses in several plant species, such as A. thaliana (Mao et al, 2016), rice (Oono et al, 2014), potato (Zhang N. et al, 2014), Brassica juncea (Bhardwaj et al, 2015), and Robinia pseudoacacia L. ‘Idaho’ (Xiu et al, 2015). Transcriptome analysis of S. miltiorrhiza identified a number of DEGs between the AtDREB1C transgenic lines and WT, suggesting that their corresponding proteins play specific roles in the drought stress response.…”

Section: Discussionmentioning

confidence: 99%

Modulating AtDREB1C Expression Improves Drought Tolerance in Salvia miltiorrhiza

et al. 2017

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Dehydration responsive element binding proteins are transcription factors of the plant-specific AP2 family, many of which contribute to abiotic stress responses in several plant species. We investigated the possibility of increasing drought tolerance in the traditional Chinese medicinal herb, Salvia miltiorrhiza, through modulating the transcriptional regulation of AtDREB1C in transgenic plants under the control of a constitutive (35S) or drought-inducible (RD29A) promoter. AtDREB1C transgenic S. miltiorrhiza plants showed increased survival under severe drought conditions compared to the non-transgenic wild-type (WT) control. However, transgenic plants with constitutive overexpression of AtDREB1C showed considerable dwarfing relative to WT. Physiological tests suggested that the higher chlorophyll content, photosynthetic capacity, and superoxide dismutase, peroxidase, and catalase activity in the transgenic plants enhanced plant drought stress resistance compared to WT. Transcriptome analysis of S. miltiorrhiza following drought stress identified a number of differentially expressed genes (DEGs) between the AtDREB1C transgenic lines and WT. These DEGs are involved in photosynthesis, plant hormone signal transduction, phenylpropanoid biosynthesis, ribosome, starch and sucrose metabolism, and other metabolic pathways. The modified pathways involved in plant hormone signaling are thought to be one of the main causes of the increased drought tolerance of AtDREB1C transgenic S. miltiorrhiza plants.

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ZmNAC55, a maize stress-responsive NAC transcription factor, confers drought resistance in transgenic Arabidopsis

Cited by 96 publications

References 46 publications

Comprehensive analysis and discovery of drought-related NAC transcription factors in common bean

Comprehensive analysis and discovery of drought-related NAC transcription factors in common bean

The Soybean GmNAC019 Transcription Factor Mediates Drought Tolerance in Arabidopsis in an Abscisic Acid-Dependent Manner

Modulating AtDREB1C Expression Improves Drought Tolerance in Salvia miltiorrhiza

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