ZmIBH1-1 regulates plant architecture in maize

Cao, Yingying; Zeng, Haixia; Ku, Lixia; Ren, Zhenzhen; Han, Yun; Su, Huihui; Dou, Deqiang; Liu, Huafeng; Dong, Yan; Zhu, Fangfang; Li, Tianyi; Zhao, Qiannan; Chen, Yanhui

doi:10.1093/jxb/eraa052

Cited by 44 publications

(23 citation statements)

References 65 publications

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“…Library DNA was checked for concentration and size distribution in an Agilent 2,100 Bioanalyzer before sequencing with an Illumina HiSeq4000 system according to the manufacturer's instructions. The RNA‐seq data analysis was performed according to the method described by Cao et al (2020). To validate the expression differences between WT and ZmNST3 observed by RNA‐Seq, quantitative real‐time PCR (qPCR) of the randomly selected 20 DEGs was performed using the SYBR Green CR Master Mix kit (Applied Biosystems, Waltham, MA), following the manufacturer's protocol on a LightCycler 480II Sequence Detection System.…”

Section: Methodsmentioning

confidence: 99%

Functions and regulatory framework of ZmNST3 in maize under lodging and drought stress

Ren

Zhang

Cao

et al. 2020

Plant Cell & Environment

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The growth and development of maize are negatively affected by various abiotic stresses including drought, high salinity, extreme temperature, and strong wind. Therefore, it is important to understand the molecular mechanisms underlying abiotic stress resistance in maize. In the present work, we identified that a novel NAC transcriptional factor, ZmNST3, enhances maize lodging resistance and drought stress tolerance. ChIP-Seq and expression of target genes analysis showed that ZmNST3 could directly regulate the expression of genes related to cell wall biosynthesis which could subsequently enhance lodging resistance. Furthermore, we also demonstrated that ZmNST3 affected the expression of genes related to the synthesis of antioxidant enzyme secondary metabolites that could enhance drought resistance. More importantly, we are the first to report that ZmNST3 directly binds to the promoters of CESA5 and Dynamin-Related Proteins2A (DRP2A) and activates the expression of genes related to secondary cell wall cellulose biosynthesis. Additionally, we revealed that ZmNST3 directly binds to the promoters of GST/GlnRS and activates genes which could enhance the production of antioxidant enzymes in vivo. Overall, our work contributes to a comprehensive understanding of the regulatory network of ZmNST3 in regulating maize lodging and drought stress resistance.

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Section: Methodsmentioning

confidence: 99%

Functions and regulatory framework of ZmNST3 in maize under lodging and drought stress

Ren

Zhang

Cao

et al. 2020

Plant Cell & Environment

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“…In maize, a bHLH transcription factor ZmIBH1-1 is a negative regulator of LA in maize. Transcriptome analysis suggested that the ZmIBH1-1-mediated LA in the leaf ligular region was highly correlated with cytokinin (CK), JA, and ethylene synthesis and signal transduction pathways associated genes, in particular the two CK responsive genes, GRMZM2G145280 (BBC1) and GRMZM2G149952 (ZmAS1) that were tightly correlated with cell division, implying that CK may modulate LA through the control of cell profile [85]. Notably, it has been demonstrated that CK indirectly interacted with BR through auxin pathway.…”

Section: Other Phytohormones Involved In Regulation Of Lamina Jointmentioning

confidence: 99%

Synergistic Interaction of Phytohormones in Determining Leaf Angle in Crops

Lü

et al. 2020

IJMS

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Leaf angle (LA), defined as the angle between the plant stem and leaf adaxial side of the blade, generally shapes the plant architecture into a loosen or dense structure, and thus influences the light interception and competition between neighboring plants in natural settings, ultimately contributing to the crop yield and productivity. It has been elucidated that brassinosteroid (BR) plays a dominant role in determining LA, and other phytohormones also positively or negatively participate in regulating LA. Accumulating evidences have revealed that these phytohormones interact with each other in modulating various biological processes. However, the comprehensive discussion of how the phytohormones and their interaction involved in shaping LA is relatively lack. Here, we intend to summarize the advances in the LA regulation mediated by the phytohormones and their crosstalk in different plant species, mainly in rice and maize, hopefully providing further insights into the genetic manipulation of LA trait in crop breeding and improvement in regarding to overcoming the challenge from the continuous demands for food under limited arable land area.

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“…Tian et al (2019) mapped 12 QTLs for leaf angle in a population of 866 maize–teosinte BC 2 S 3 recombinant inbred lines derived from a cross between the maize inbred line W22 and the teosinte accession CIMMYT 8759. To date, in the multiple QTLs that have been located, only six genes have been reported as a result of the combined use of quantitative genetics (Ku et al , 2011; Zhang et al ., 2014; Ren et al , 2020; Tian et al ., 2019; Cao et al ., 2020). The first reported candidate gene, ZmTAC1 , on a major QTL for LA that was detected on chromosome 2, was cloned using a comparative genomics method (Ku et al , 2011).…”

Section: Introductionmentioning

confidence: 99%

“…(2020) also found that ZmILI1 and CYP90D1 formed a negative feedback loop to maintain the balance of brassinolide in maize. Cao et al . (2020) demonstrated that ZmIBH1-1 cloned by a map-based method negatively regulated leaf angle by causing cell wall lignification and cell elongation in the ligular region in maize.…”

Section: Introductionmentioning

confidence: 99%

ZmCLA4 regulates leaf angle through multiple plant hormone-mediated signal pathways in maize

Dou

Han

et al. 2020

Preprint

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Leaf angle in cereals is an important agronomic trait contributing to plant architecture and grain yield by determining the plant compactness. Although ZmCLA4 was identified to shape plant architecture by affecting leaf angle, the detailed regulatory mechanism of ZmCLA4 in maize remains unclear. ZmCLA4 was identified as a transcriptional repressor using the Gal4-LexA/UAS system and transactivation analysis in yeast. The DNA affinity purification (DAP)-seq assay showed that ZmCLA4 not only acts as a repressor containing the EAR motif (CACCGGAC), but was also found to have two new motifs, CCGARGS and CDTCNTC. On analyzing the ZmCLA4-bound targeted genes, we found that ZmCLA4, as a cross node of multiple plant hormone-mediated pathways, directly bound to ARF22 and IAA26 to regulate auxin transport and mediated brassinosteroid signaling by directly binding to BZR3 and 14-3-3. ZmCLA4 bound two WRKY genes involved with abscisic acid, two genes (CYP75B1, CYP93D1) involved with jasmonic acid, B3 involved in the response to ethylene, and thereby negatively regulated leaf angle formation. We built a new regulatory network for the ZmCLA4 gene controlling leaf angle in maize, which contributed to the understanding of ZmCLA4’s regulatory mechanism and will improve grain yields by facilitating optimization of plant architecture.

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ZmIBH1-1 regulates plant architecture in maize

Cited by 44 publications

References 65 publications

Functions and regulatory framework of ZmNST3 in maize under lodging and drought stress

Functions and regulatory framework of ZmNST3 in maize under lodging and drought stress

Synergistic Interaction of Phytohormones in Determining Leaf Angle in Crops

ZmCLA4 regulates leaf angle through multiple plant hormone-mediated signal pathways in maize

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