ZmGRF, a GA regulatory factor from maize, promotes flowering and plant growth in Arabidopsis

Xu, Miaoyun; Lu, Yanjin; Yang, Hongmei; He, Jingcheng; Hu, Zhiqiu; Hu, Xiaolong; Luan, Mingda; Zhang, Lan; Fan, Yanting; Wang, Lei

doi:10.1007/s11103-014-0267-9

Cited by 13 publications

(13 citation statements)

References 27 publications

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“…For example, GA2ox , which functions in converting active GAs to inactive forms (Sun, ), was up‐regulated in GhWIP2OE plants. Conversely, GRF1 , a homolog of ZmGRF , which promotes the accumulation of active GA by increasing the expression of KO1 in maize (Xu et al ., ), was down‐regulated in GhWIP2OE plants. Homologs of genes known to be related to the GA signaling pathway, such as AtZFP8 (Gan et al ., ), were down‐regulated in GhWIP2OE (Fig.…”

Section: Discussionmentioning

confidence: 93%

“…Plant morphogenesis is regulated by coordinated activation of cell differentiation, cell division, and cell expansion; obviously, organ growth is one of best reflections of these processes (Mizukami, ). At the cellular level, organ growth occurs by initial cell proliferation in which cell number increase, followed by cell expansion, which is critically important for the final shape and size of all plant organs (Li et al ., ; Xu et al ., ; Zhang et al ., ; Velasquez et al ., ). Molecular mechanisms that control the cell expansion are not well understood.…”

Section: Introductionmentioning

confidence: 99%

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GhWIP2, a WIP zinc finger protein, suppresses cell expansion in Gerbera hybrida by mediating crosstalk between gibberellin, abscisic acid, and auxin

Ren

Huang

et al. 2018

New Phytologist

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Cell expansion is a key determinant for the final size and shape of plant organ, and is regulated by various phytohormones. Zinc finger proteins (ZFPs) consist of a superfamily involved in multiple aspects of organ morphogenesis. However, little is known about WIP-type ZFP function in phytohormone-mediated organ growth. Using reverse genetics, RNA-seq and phytohormone quantification, we elucidated the role of a new WIP-type ZFP from Gerbera hybrida, GhWIP2, in controlling organ growth via regulation of cell expansion. GhWIP2 localizes to the nucleus and acts as a transcriptional repressor. Constitutive overexpression of GhWIP2 (GhWIP2OE) in both Gerbera and Arabidopsis thaliana caused major developmental defects associated with cell expansion, including dwarfism, short petals, scapes, and petioles. Furthermore, GhWIP2OE plants were hypersensitive to GA, but not to ABA, and showed a reduction in endogenous GA and auxin, but not ABA concentrations. Consistent with these observations, RNA-seq analysis revealed that genes involved in GA and auxin signaling were down-regulated, while those involved in ABA signaling were up-regulated in GhWIP2OE plants. Our findings suggest that GhWIP2 acts as a transcriptional repressor, suppressing cell expansion during organ growth by modulating crosstalk between GA, ABA, and auxin.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

GhWIP2, a WIP zinc finger protein, suppresses cell expansion in Gerbera hybrida by mediating crosstalk between gibberellin, abscisic acid, and auxin

Ren

Huang

et al. 2018

New Phytologist

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“…The process from ent-kaurene to ent-kaurene acid is catalyzed by AtKO1 , which belongs to the cytochrome P450 CYP701A subfamily (Regnault et al, 2014 ). The up-regulation of AtKO1 in transgenic Arabidopsis resulted in an increase in GA 4 content (Xu et al, 2015 ). KAO1 is highly expressed in the seeds and flowers of Arabidopsis , while its expression is low in seedlings and vegetative tissues (Xu et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic Regulation of GA Metabolism during Vernalization, Floral Bud Initiation and Development in Pak Choi (Brassica rapa ssp. chinensis Makino)

et al. 2017

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Pak choi (Brassica rapa ssp. chinensis Makino) is a representative seed vernalization vegetable and premature bolting in spring can cause significant economic loss. Thus, it is critical to elucidate the mechanism of molecular regulation of vernalization and floral bud initiation to prevent premature bolting. Gibberellin (GA) is the key plant hormone involved in regulating plant development. To gain a better understanding of GA metabolism in pak choi, the content of GA in pak choi was measured at different stages of plant development using enzyme-linked immunosorbent assay. The results showed that the GA content increased significantly after low-temperature treatment (4°C) and then decreased rapidly with vegetative growth. During floral bud initiation, the GA content increased rapidly until it peaked upon floral bud differentiation. To elucidate these changes in GA content, the expression of homologous genes encoding enzymes directly involved in GA metabolism were analyzed. The results showed that the changes in the expression of four genes involved in GA synthesis (Bra035120 encoding ent-kaurene synthase, Bra009868 encoding ent-kaurene oxidase, Bra015394 encoding ent-kaurenoic acid oxidase, and Bra013890 encoding GA20-oxidase) were correlated with the changes in GA content. In addition, by comparing the expression of genes involved in GA metabolism at different growth stages, seven differentially expressed genes (Bra005596, Bra009285, Bra022565, Bra008362, Bra033324, Bra010802, and Bra030500) were identified. The differential expression of these genes were directly correlated with changes in GA content, suggesting that these genes were directly related to vernalization, floral bud initiation and development. These results contribute to the understanding of the molecular mechanism of changes in GA content during different developmental phases in pak choi.

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“…Briefly, AsA is indirectly involved in floral induction due to its interaction with ABA and GA production (Barth et al, 2006). It is well evident that GA promotes flowering (Yamaguchi et al, 2014; Xu et al, 2015), inhibits senescence (Currey et al, 2013), antagonizes the effect of salicylic, jasmomic acid and ABA, and shows a complex interplay among hormones in order to mediate signaling which can be in the form of a network (He et al, 2001; Navabpour et al, 2003; Barth et al, 2006). …”

Section: Ascorbate-hormone Crosstalkmentioning

confidence: 99%

Ascorbic Acid-A Potential Oxidant Scavenger and Its Role in Plant Development and Abiotic Stress Tolerance

2017

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Over-production of reactive oxygen species (ROS) in plants under stress conditions is a common phenomenon. Plants tend to counter this problem through their ability to synthesize ROS neutralizing substances including non-enzymatic and enzymatic antioxidants. In this context, ascorbic acid (AsA) is one of the universal non-enzymatic antioxidants having substantial potential of not only scavenging ROS, but also modulating a number of fundamental functions in plants both under stress and non-stress conditions. In the present review, the role of AsA, its biosynthesis, and cross-talk with different hormones have been discussed comprehensively. Furthermore, the possible involvement of AsA-hormone crosstalk in the regulation of several key physiological and biochemical processes like seed germination, photosynthesis, floral induction, fruit expansion, ROS regulation and senescence has also been described. A simplified and schematic AsA biosynthetic pathway has been drawn, which reflects key intermediates involved therein. This could pave the way for future research to elucidate the modulation of plant AsA biosynthesis and subsequent responses to environmental stresses. Apart from discussing the role of different ascorbate peroxidase isoforms, the comparative role of two key enzymes, ascorbate peroxidase (APX) and ascorbate oxidase (AO) involved in AsA metabolism in plant cell apoplast is also discussed particularly focusing on oxidative stress perception and amplification. Limited progress has been made so far in terms of developing transgenics which could over-produce AsA. The prospects of generation of transgenics overexpressing AsA related genes and exogenous application of AsA have been discussed at length in the review.

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ZmGRF, a GA regulatory factor from maize, promotes flowering and plant growth in Arabidopsis

Cited by 13 publications

References 27 publications

GhWIP2, a WIP zinc finger protein, suppresses cell expansion in Gerbera hybrida by mediating crosstalk between gibberellin, abscisic acid, and auxin

GhWIP2, a WIP zinc finger protein, suppresses cell expansion in Gerbera hybrida by mediating crosstalk between gibberellin, abscisic acid, and auxin

Genetic Regulation of GA Metabolism during Vernalization, Floral Bud Initiation and Development in Pak Choi (Brassica rapa ssp. chinensis Makino)

Ascorbic Acid-A Potential Oxidant Scavenger and Its Role in Plant Development and Abiotic Stress Tolerance

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