ZmbZIP54 and ZmFDX5 cooperatively regulate maize seedling tolerance to lead by mediating ZmPRP1 transcription

Hou, Fengxia; Zhang, Na; Ma, Langlang; Liu, An; Zhou, Xun; Zou, Chaoying; Yang, Cong; Pan, Guangtang; Lübberstedt, Thomas; Shen, Yaou

doi:10.1016/j.ijbiomac.2022.10.151

Cited by 8 publications

(3 citation statements)

References 69 publications

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“…9a). Our previous study demonstrated that ZmbZIP54 was a key transcript factor in responses to Pb stress, which regulated Pb tolerance and accumulation in maize seedlings (Hou et al 2022b). Subsequently, we used Y1H to test whether ZmbZIP54 bound to the ZmNRAMP6 promoter.…”

Section: Zmnramp6 Being Regulated By the Pb Tolerance-related Transcr...mentioning

confidence: 99%

“…Owing to its good adaptability to lowfertility soil, fast growth, and high bioaccumulation, maize has been used as an optimum phytoremediation crop for heavy metal-contaminated soil (Wang et al 2007). A number of studies have shown that maize accumulates more Pb in its roots and transports only a small amount to its aboveground parts (Dai et al 2007; Ma et al 2022;Hou et al 2022b). This enables maize to aid in soil phytoremediation and energy supply, in consideration of its food security (Li et al 2023).…”

Section: Introductionmentioning

confidence: 99%

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A genome-wide co-expression network analysis revealed ZmNRAMP6-mediated regulatory pathway involved in maize tolerance to lead stress

et al. 2023

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Lead (Pb) is one of the most toxic heavy metal pollutants, which can penetrate to plant cells via root absorption and thus cause irreversible damages to the human body through the food chain. To identify the hub gene responsible for Pb tolerance in maize, we performed a trait-associated co-expression network analysis at a genome-wide level, using two maize lines with contrasting Pb tolerances. Finally, ZmNRAMP6 that encodes a metal transporter was identi ed as the hub gene among the Pb toleranceassociated co-expression module. Heterologous expression of ZmNRAMP6 in yeast veri ed its role in Pb transport. Combined Arabidopsis overexpression and maize mutant analysis suggested that ZmNRAMP6 conferred plant sensitivity to Pb stress by transporting Pb ion from the roots to shoots. Knockout of ZmNRAMP6 caused Pb retention in the roots and activation of the antioxidant enzyme system, resulting in an increased Pb tolerance in maize. An integration of yeast one-hybrid and dual-luciferase reporter assay uncovered that ZmNRAMP6 was negatively regulated by a known Pb tolerance-related transcript factor ZmbZIP54. Different from the other known NRAMP genes, ZmNRAMP6 acted as a Pb transporter from the roots to shoots and environment. Collectively, knockout of ZmNRAMP6 will aid in the bioremediation of contaminated soil and food safety guarantee of forage and grain corn. Key MessageA metal transporter ZmNRAMP6 was identi ed by using a trait-associated co-expression network analysis at a genome-wide level. ZmNRAMP6 confers maize sensitivity to Pb by accumulating it to maize shoots. ZmNRAMP6 knockout detains Pb in roots, activates antioxidant enzymes, and improves Pb tolerance.

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Section: Zmnramp6 Being Regulated By the Pb Tolerance-related Transcr...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A genome-wide co-expression network analysis revealed ZmNRAMP6-mediated regulatory pathway involved in maize tolerance to lead stress

et al. 2023

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“…Gao et al found that many transcription factor families, such as bZIP, ERF, and GARP, can respond to Pb stress in maize [44]. Zhang et al verified the positive regulatory effects of several bZIP genes on Pb tolerance in maize [45], and Hou et al also found that the ZmbZIP54 gene could regulate Pb tolerance in maize by targeting the ZmPRP1 gene [46]. The Pb resistance function of more transcription factors needs to be verified in the future.…”

Section: Target Pathway Prediction Analysis Of 7 Candidate Sinacsmentioning

confidence: 99%

Transcriptome-Wide Identification and Response Pattern Analysis of the Salix integra NAC Transcription Factor in Response to Pb Stress

Yue

Huang

et al. 2023

IJMS

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The NAC (NAM-ATAF1/2-CUC) transcription factor family is one of the largest plant-specific transcription factor families, playing an important role in plant growth and development and abiotic stress response. As a short-rotation woody plant, Salix integra (S. integra) has high lead (Pb) phytoremediation potential. To understand the role of NAC in S. integra Pb tolerance, 53 SiNAC transcripts were identified using third-generation and next-generation transcriptomic data from S. integra exposed to Pb stress, and a phylogenetic analysis revealed 11 subfamilies. A sequence alignment showed that multiple subfamilies represented by TIP and ATAF had a gene that produced more than one transcript under Pb stress, and different transcripts had different responses to Pb. By analyzing the expression profiles of SiNACs at 9 Pb stress time points, 41 of 53 SiNACs were found to be significantly responsive to Pb. Short time-series expression miner (STEM) analysis revealed that 41 SiNACs had two significant Pb positive response patterns (early and late), both containing 10 SiNACs. The SiNACs with the most significant Pb response were mainly from the ATAF and NAP subfamilies. Therefore, 4 and 3 SiNACs from the ATAF and NAP subfamilies, respectively, were selected as candidate Pb-responsive SiNACs for further structural and functional analysis. The RT-qPCR results of 7 transcripts also confirmed the different Pb response patterns of the ATAF and NAP subfamilies. SiNAC004 and SiNAC120, which were randomly selected from two subfamilies, were confirmed to be nuclear localization proteins by subcellular localization experiments. Functional prediction analysis of the associated transcripts of seven candidate SiNACs showed that the target pathways of ATAF subfamily SiNACs were “sulfur metabolism” and “glutathione metabolism”, and the target pathways of NAP subfamily SiNACs were “ribosome” and “phenylpropanoid biosynthesis”. This study not only identified two NAC subfamilies with different Pb response patterns but also identified Pb-responsive SiNACs that could provide a basis for subsequent gene function verification.

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A combination of QTL mapping and genome-wide association study revealed the key gene for husk number in maize

Liang,

Xi,

Liu

et al. 2024

Theor Appl Genet

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ZmbZIP54 and ZmFDX5 cooperatively regulate maize seedling tolerance to lead by mediating ZmPRP1 transcription

Cited by 8 publications

References 69 publications

A genome-wide co-expression network analysis revealed ZmNRAMP6-mediated regulatory pathway involved in maize tolerance to lead stress

A genome-wide co-expression network analysis revealed ZmNRAMP6-mediated regulatory pathway involved in maize tolerance to lead stress

Transcriptome-Wide Identification and Response Pattern Analysis of the Salix integra NAC Transcription Factor in Response to Pb Stress

A combination of QTL mapping and genome-wide association study revealed the key gene for husk number in maize

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