ZmASR3 from the Maize ASR Gene Family Positively Regulates Drought Tolerance in Transgenic Arabidopsis

Liang, Yani; Jiang, Yingli; Du, Ming; Li, Baoyan; Chen, Long; Chen, Mingchao; Jin, Demiao; Wu, Jiandong

doi:10.3390/ijms20092278

Cited by 38 publications

(34 citation statements)

References 58 publications

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“…These lines had lower malondialdehyde content and higher ABA and proline content improving their drought tolerance. The results thus, proved Zm ASR3 enhance drought tolerance via an ABA dependent pathway [82]. Their precise role in conferring improved tolerance to drought and salt has also been established in tomato, rice and lily [33,34,26].…”

Section: Plos Onementioning

confidence: 58%

“…Maize ASRs also have a Zn-binding domain at the N-terminal and a nuclear targeting signal with two abscissic acid/water deficit stress domains (ABA/WDS) at the C-terminal. Hybrid assays and sub-cellular fractionation studies confirmed the role of ZmASR proteins as transcription factors or molecular chaperons in different plant species [ 61 ].…”

Section: Discussionmentioning

confidence: 95%

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Characterization of ASR gene and its role in drought tolerance in chickpea (Cicer arietinum L.)

et al. 2020

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Chickpea has a profound nutritional and economic value in vegetarian society. Continuous decline in chickpea productivity is attributed to insufficient genetic variability and different environmental stresses. Chickpea like several other legumes is highly susceptible to terminal drought stress. Multiple genes control drought tolerance and ASR gene plays a key role in regulating different plant stresses. The present study describes the molecular characterization and functional role of Abscissic acid and stress ripening (ASR) gene from chickpea (Cicer arietinum) and the gene sequence identified was submitted to NCBI Genbank (MK937569). Molecular analysis using MUSCLE software proved that the ASR nucleotide sequences in different legumes show variations at various positions though ASR genes are conserved in chickpea with only few variations. Sequence similarity of ASR gene to chickpea putative ABA/WDS induced protein mRNA clearly indicated its potential involvement in drought tolerance. Physiological screening and qRT-PCR results demonstrated increased ASR gene expression under drought stress possibly enabled genotypes to perform better under stress. Conserved domain search, protein structure analysis, prediction and validation, network analysis using Phyre2, Swiss-PDB viewer, ProSA and STRING analysis established the role of hypothetical ASR protein NP_001351739.1 in mediating drought responses. NP_001351739.1 might have enhanced the ASR gene activity as a transcription factor regulating drought stress tolerance in chickpea. This study could be useful in identification of new ASR genes that play a major role in drought tolerance and also develop functional markers for chickpea improvement.

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Section: Plos Onementioning

confidence: 58%

Section: Discussionmentioning

confidence: 95%

Characterization of ASR gene and its role in drought tolerance in chickpea (Cicer arietinum L.)

et al. 2020

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“…Most of the ASRs in foxtail millet, maize and rice were ubiquitously expressed in all tested tissues, suggesting the wide functioning of ASRs in many development processes in cereal crops [16,37]. The TF-binding sites analysis suggested that most TaASR genes were involved in various processes during growth and development.…”

Section: Discussionmentioning

confidence: 93%

“…Also, it has been documented that overexpression of LLA23 gene in Arabidopsis conferred the cold and freezing tolerance [39]. Likewise, overexpression plantain MpASR and maize ZmASR3 in Arabidopsis, tomato SlASR1, Brachypodium distachyon BdASR1 and wheat ASR1 in tobacco all improved the tolerance to water stress [8,21,37,[40][41][42]. In addition, transgenic rice plants with overexpression of OsASR2 enhanced the tolerance to drought by targeting the GT-1 cis-element [35], while overexpression of OsASR5 enhanced the drought tolerance by regulating ABA synthesis, promoting stomatal closure, and acting as chaperone-like protein [34].…”

mentioning

confidence: 97%

“…Increasing evidences showed that ASR proteins play important roles in plant growth and fruit ripening [1,[25][26][27], as well as in regulation of floral development and flowering time [15,28,29]. The ASR proteins have been well documented for their responses to multiple abiotic stresses, including drought, salt, heat, cold, and exposure to cadmium (Cd) and aluminum (Al) [9,24,[30][31][32][33][34][35][36][37][38].…”

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confidence: 99%

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Genome-wide characterization of the abscisic acid-, stress- and ripening-induced (ASR) gene family in wheat (Triticum aestivum L.)

Guan²,

Zhuo

et al. 2020

Biol Res

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Background: Abscisic acid-, stress-, and ripening-induced (ASR) genes are a class of plant specific transcription factors (TFs), which play important roles in plant development, growth and abiotic stress responses. The wheat ASRs have not been described in genome-wide yet. Methods: We predicted the transmembrane regions and subcellular localization using the TMHMM server, and Plant-mPLoc server and CELLO v2.5, respectively. Then the phylogeny tree was built by MEGA7. The exon-intron structures, conserved motifs and TFs binding sites were analyzed by GSDS, MEME program and PlantRegMap, respectively. Results: In wheat, 33ASR genes were identified through a genome-wide survey and classified into six groups. Phylogenetic analyses revealed that the TaASR proteins in the same group tightly clustered together, compared with those from other species. Duplication analysis indicated that the TaASR gene family has expanded mainly through tandem and segmental duplication events. Similar gene structures and conserved protein motifs of TaASRs in wheat were identified in the same groups. ASR genes contained various TF binding cites associated with the stress responses in the promoter region. Gene expression was generally associated with the expected group-specific expression pattern in five tissues, including grain, leaf, root, spike and stem, indicating the broad conservation of ASR genes function during wheat evolution. The qRT-PCR analysis revealed that several ASRs were up-regulated in response to NaCl and PEG stress. Conclusion: We identified ASR genes in wheat and found that gene duplication events are the main driving force for ASR gene evolution in wheat. The expression of wheat ASR genes was modulated in responses to multiple abiotic stresses, including drought/osmotic and salt stress. The results provided important information for further identifications of the functions of wheat ASR genes and candidate genes for high abiotic stress tolerant wheat breeding.

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Expression patterns of the ZmASR family during simulated drought stress in maize

Zhang

Guo

et al. 2021

Agronomy Journal

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Maize (Zea mays L.) is an important food crop. Maize production is adversely affected by abiotic stress, especially drought stress. The screening and characterization of the roles of stress-related genes in maize are thus essential for improving abiotic stress resistance. Abscisic acid-stress-ripening (ASR)-induced proteins with abscisic acid/water deficit stress (ABA/WDS) domains are a class of plant-specific transcription factors that play an important role in plant development, growth, and abiotic stress responses. In this study, nine members of the ASR-induced protein in maize (ZmASR) family were predicted and analyzed in maize in terms of gene structure, subcellular localization, amino acid sequence, physical and chemical properties, secondary structure, tertiary structure, and phosphorylation modification sites by bioinformatics. Analysis of regulatory promoter elements was performed to provide additional information about their distinctive tissue-specific expression and possible functions. To further investigate the function of these proteins in maize, the expression levels of ZmASRs in different tissues and in response to different drought stress treatments were analyzed using quantitative real-time polymerase chain reaction (qRT-PCR). The results showed that the nine members of the maize ZmASR family have very different expression levels in different organs and under different degrees of drought stress. Moreover, the expression levels of the nine members in the same organ also varied greatly with leaf age. These findings provide a reference for functional research of the maize ZmASR gene family. Our findings have important theoretical and practical significance for cultivating new drought-resistant maize varieties and promoting the development of the maize industry.

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ZmASR3 from the Maize ASR Gene Family Positively Regulates Drought Tolerance in Transgenic Arabidopsis

Cited by 38 publications

References 58 publications

Characterization of ASR gene and its role in drought tolerance in chickpea (Cicer arietinum L.)

Characterization of ASR gene and its role in drought tolerance in chickpea (Cicer arietinum L.)

Genome-wide characterization of the abscisic acid-, stress- and ripening-induced (ASR) gene family in wheat (Triticum aestivum L.)

Expression patterns of the ZmASR family during simulated drought stress in maize

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