Ziziphora clinopodioides ameliorated rheumatoid arthritis and inflammatory paw edema in different models of acute and chronic inflammation

Shabbir, Arham; Batool, Syeda Amina; Basheer, Muhammad Irfan; Shahzad, Muhammad; Sultana, Kishwar; Tareen, Rasool Baksh; Iqbal, Javed; Saeed-Ul-Hassan,

doi:10.1016/j.biopha.2017.11.118

Cited by 62 publications

(42 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The anti-inflammatory activity of the MEDP was evaluated according to the method previously described (Shabbir et al. 2018). The paw oedema was induced by sub-plantar administration of 0.1 mL of freshly prepared solutions of histamine (0.5%) or serotonin (5-HT) (0.5%).…”

Section: Methodsmentioning

confidence: 99%

Assessment of the antinociceptive and anti-inflammatory activities of the stem methanol extract of Diplotropis purpurea

Cruz

Díaz

Gupta

et al. 2019

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Since there is still a great need to search for plant species with antinociceptive and anti-inflammatory activities, Diploptropis purpurea (Rich.) Amshoff (Fabaceae) is studied for the first time. Objective: This evaluates the analgesic and anti-inflammatory activities of the stem methanol extract of Diplotropis purpurea (MEDP). Material and methods: The anti-inflammatory and analgesic effects of MEDP of D. purpurea were evaluated in vivo . The antinociceptive activity was assessed in CD1 male mice were treated by oral gavage with 500 mg/kg of MEDP 30 min before submitting to acetic acid-induced abdominal writhing, hot-plate, and formalin tests. Paws oedema induced by carrageenan, histamine or serotonin were performed in male Sprague–Dawley rats to determinate the anti-inflammatory activity. Results: Oral administration of MEDP produced significant antinociceptive effects on the inflammatory phase in the formalin test [12.0 s versus 72.5 s in carboxymethyl cellulose (CMC) control group]. MEDP produced an analgesic effect in the hot-plate model, although the effect was modest compared to tramadol (40 and 60%, respectively). The oral administration of MEDP in a dose of 500 mg/kg showed maximum inhibition (75.1%) after 0.5 h in carrageenan-induced oedema, but it did not modify histamine or serotonin-induced oedemas. Discussion and conclusion: In the peripheral nociception model, acetic acid-induced abdominal writhing, the MEDP did not show a protective effect, but its analgesic effects were evident in the inflammatory phase of the formalin test and in the hot-plate model. These results show that the anti-inflammatory effect was accompanied by a reduction in the perception of painful stimuli.

show abstract

Section: Methodsmentioning

confidence: 99%

Assessment of the antinociceptive and anti-inflammatory activities of the stem methanol extract of Diplotropis purpurea

Cruz

Díaz

Gupta

et al. 2019

Pharmaceutical Biology

View full text Add to dashboard Cite

show abstract

“…Scores 0–4 were given to normal-severe edema and/or erythema in ipsilateral paw. Arthritic score crossing 4 is the indication of involvement of contralateral paw [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

Amelioration of Rheumatoid Arthritis by Anacardium occidentale via Inhibition of Collagenase and Lysosomal Enzymes

Naz

Ahmed

Shahzad

et al. 2020

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

Anacardium occidentale (cashew) has been used in the traditional system of medicine for curing many inflammatory disorders. The present study investigates the antiarthritic effects of cashew leaves extract using the rat model of FCA-induced rheumatoid arthritis. Arthritic rats were treated with 100 and 200 mg/kg b.w. ethanolic extract of cashew leaves. Animals were sacrificed at day 23, and before sacrificing the animals, gross pathological changes were observed. Histopathology of ankle joint was evaluated with hematoxylin and eosin staining, whereas the serum levels of C-reactive protein (CRP) were evaluated by the agglutination method. Inflammatory cells and other hematological parameters were assessed by employing an automated hemocytometer and chemistry analyzer. Rheumatoid factor (Rf) and lysosomal enzymes levels were determined in blood. Results indicated that A. occidentale significantly decreased the CPR levels, macroscopic arthritic score, and rheumatoid factor as compared to the diseased group. Histopathological evaluation showed significant attenuation in bone erosion, joint inflammation, and pannus formation by plant extract. Treatment with A. occidentale significantly suppressed the levels of acid phosphatase, β-galactosidase, β-glucuronidase, N-acetylglucosaminidase, and collagenase. Moreover, A. occidentale significantly raised the HB levels and RBCs counts which were found depleted in the diseased group. The raised counts of total leukocytes, platelets, neutrophils, lymphocytes, and monocytes were also significantly decreased by treatment with plant extract. Comparative analysis showed that higher dose of A. occidentale demonstrated superior amelioration of rheumatoid arthritis as compared to low dose. In conclusion, A. occidentale possesses significant antiarthritic potential, which may be attributed to the suppression of lysosomal enzymes and collagenase levels.

show abstract

“…The percentage of paw edema suppression was calculated from the average difference in paw volume in the experimental group treated and in the control group using the following formula [96]: After micro-CT scanning, the animal paws were fixed in 10% buffered formalin solution for 72 hours. They were decalcified in a decalcifying solution of SoftiDec (Biovitrum, St. Petersburg, Russia) for 30 days.…”

Section: Micro-ct Analysismentioning

confidence: 99%

Anti-Arthritic Effect of Chicken Embryo Tissue Hydrolyzate Against Adjuvant Arthritis in Rats (X-Ray Microtomographic and Histopathological Analysis)

Rzhepakovsky¹,

Avanesyan²,

Benlidayı³

et al. 2020

Preprint

View full text Add to dashboard Cite

Finding new, safe strategies to prevent and control rheumatoid arthritis is an urgent task. Of particular interest in this regard are bioactive peptides and peptide-rich protein hydrolyzates, which represent a new trend in the development of functional foods and nutraceuticals. The resulting tissue hydrolyzate of the chicken embryo (CETH) has been evaluated for acute toxicity and tested against chronic arthritis induced by Freund's full adjuvant in rats. The anti-arthritic effect of CETH was studied on the 28th day of the experiment after two weeks of oral administration of CETH at doses of 60 and 120 mg/kg body weight. Arthritis was evaluated on the last day of the experiment on the injected animal paw using X-ray computerized microtomography and histopathology analysis methods. The CETH effect was compared with the non-steroidal anti-inflammatory drug diclofenac sodium (5 mg/kg). Oral administration of CETH was accompanied by effective dose-dependent correction of morphological changes caused by the adjuvant injection. CETH had relatively high recovery effects in terms of parameters for reducing inflammatory edema, inhibition of osteolysis, prevention of osteophitosis, reduction of the inflammatory reaction of periarticular tissues, and cartilage degeneration. This study presents a potential theoretical strategy for the safe correction of this pathological process and, for the first time, shows that CETH may be a powerful potential nutraceutical agent or bioactive component of functional products in the treatment of rheumatoid arthritis.

show abstract

Ziziphora clinopodioides ameliorated rheumatoid arthritis and inflammatory paw edema in different models of acute and chronic inflammation

Cited by 62 publications

References 39 publications

Assessment of the antinociceptive and anti-inflammatory activities of the stem methanol extract of Diplotropis purpurea

Assessment of the antinociceptive and anti-inflammatory activities of the stem methanol extract of Diplotropis purpurea

Amelioration of Rheumatoid Arthritis by Anacardium occidentale via Inhibition of Collagenase and Lysosomal Enzymes

Anti-Arthritic Effect of Chicken Embryo Tissue Hydrolyzate Against Adjuvant Arthritis in Rats (X-Ray Microtomographic and Histopathological Analysis)

Contact Info

Product

Resources

About