Zippered release from polymer-gated carbon nanotubes

Mashat, Afnan; Deng, Lin; Altawashi, Azza; Sougrat, Rachid; Wang, Guangchao; Khashab, Niveen M.

doi:10.1039/c2jm30454f

Cited by 16 publications

(9 citation statements)

References 61 publications

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“…These properties allow polymer-coated CNTs to be efficient transfection agents. 8,[17][18][19][20] In our previous work, we functionalized CNTs with two polyamine polymers, PEI and PAMAM. These compounds have low cytotoxicity, ability to conjugate microRNAs (such as miR-503) and, at the same time, transfect efficiently endothelial cells.…”

Section: Celluzzi Et Almentioning

confidence: 99%

“…To assess the stability of CNT/miRNA complexes, heparin 5,000 U/mL (Teva Pharmaceutical Industries, Petah Tikva, Israel) was added to CNT/ miRNA complexes, and the displacement of the complex was monitored after incubating for 30 and 60 min. To test the supernatant toxicity also, cells were treated with supPEI-CNTs and supPAM-CNTs at the same concentration of polymers (4,8,12 and 20 μg/mL) or CNTs (10,20,30 and 50 μg/mL).…”

Section: Gel Retardation Assaysmentioning

confidence: 99%

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Biophysical and biological contributions of polyamine-coated carbon nanotubes and bidimensional buckypapers in the delivery of miRNAs to human cells

Celluzzi

Paolini

D’Oria

et al. 2017

IJN

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Recent findings in nanomedicine have revealed that carbon nanotubes (CNTs) can be used as potential drug carriers, therapeutic agents and diagnostics tools. Moreover, due to their ability to cross cellular membranes, their nanosize dimension, high surface area and relatively good biocompatibility, CNTs have also been employed as a novel gene delivery vector system. In our previous work, we functionalized CNTs with two polyamine polymers, polyethyleneimine (PEI) and polyamidoamine dendrimer (PAMAM). These compounds have low cytotoxicity, ability to conjugate microRNAs (such as miR-503) and, at the same time, transfect efficiently endothelial cells. The parameters contributing to the good efficiency of transfection that we observed were not investigated in detail. In fact, the diameter and length of CNTs are important parameters to be taken into account when evaluating the effects on drug delivery efficiency. In order to investigate the biophysical and biological contributions of polymer-coated CNTs in delivery of miRNAs to human cells, we decided to investigate three different preparations, characterized by different dimensions and aspect ratios. In particular, we took into account very small CNTs, a suspension of CNTs starting from the commercial product and a 2D material based on CNTs (ie, buckypapers [BPs]) to examine the transfection efficiency of a rigid scaffold. In conclusion, we extensively investigated the biophysical and biological contributions of polyamine-coated CNTs and bidimensional BPs in the delivery of miRNAs to human cells, in order to optimize the transfection efficiency of these compounds to be employed as efficient drug delivery vectors in biomedical applications.

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Section: Celluzzi Et Almentioning

confidence: 99%

Section: Gel Retardation Assaysmentioning

confidence: 99%

Biophysical and biological contributions of polyamine-coated carbon nanotubes and bidimensional buckypapers in the delivery of miRNAs to human cells

Celluzzi

Paolini

D’Oria

et al. 2017

IJN

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“…Several CNT-based smart systems have been designed to initiate and manipulate cargo release based on abnormalities in physiological/environmental conditions. Several studies designed CNT systems in response to either endogenous factors (pH [151][152][153][195][196][197][198][199], enzymatic [148,157,166,200], redox gradients [162,164,165,171], and temperature [201]) or exogenous factors (electric [202][203][204], electromagnetic radiation [145,[205][206][207], magnetic [175] and ultrasound [170]).…”

Section: Stimuli-responsive Cnt-based Drug Delivery Systemsmentioning

confidence: 99%

Enzymatic ‘stripping’ and degradation of PEGylated carbon nanotubes

et al. 2014

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Single-walled carbon nanotubes (SWCNTs) coated or functionalized with PEG chains of different molecular weight were assessed for their propensity to undergo biodegradation under in vitro conditions using recombinant myeloperoxidase (MPO) or ex vivo using freshly isolated primary human neutrophils. Our findings suggest that under natural conditions, a combined process of 'stripping' (i.e., defunctionalization) and biodegradation of PEG-SWCNTs might occur and that PEG-SWCNTs are a promising--and degradable--nanomedicine vector.

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“…Some other DOX-loaded NPs were also constructed to realize reduced adverse effect and enhanced therapeutic efficiency. [8][9][10][11][12] These nanomedicines demonstrated diverse advantages, such as enhanced drug stability and intracellular uptake, prolonged circulation time, decreased systemic toxicity and improved antitumor efficacy, by virtue of the multifunctional materials as well as the enhanced permeability and retention (EPR) effect. However, the content of excipient in most nanomedicines can reach as high as 90%, resulting in low drug loading content (DLC) (usually less than 10%, w/w).…”

Section: Introductionmentioning

confidence: 99%

A facile doxorubicin-dichloroacetate conjugate nanomedicine with high drug loading for safe drug delivery

Yang

Wang

Qin

et al. 2018

IJN

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BackgroundDoxorubicin (DOX) is an effective chemotherapeutic agent but severe side effects limit its clinical application. Nanoformulations can reduce the toxicity while still have various limitations, such as complexity, low drug loading capability and excipient related concerns.MethodsAn amphiphilic conjugate, doxorubicin-dichloroacetate, was synthesized and the corresponding nanoparticles were prepared. The in vitro cytotoxicity and intracellular uptake, in vivo imaging, antitumor effects and systemic toxicities of nanoparticles were carried out to evaluate the therapeutic efficiency of tumor.ResultsDoxorubicin-dichloroacetate conjugate can self-assemble into nanoparticles with small amount of DSPE-PEG2000, leading to high drug loading (71.8%, w/w) and diminished excipient associated concerns. The nanoparticles exhibited invisible systemic toxicity and high maximum tolerated dose of 75 mg DOX equiv./kg, which was 15-fold higher than that of free DOX. It also showed good tumor targeting capability and enhanced antitumor efficacy in murine melanoma model.ConclusionThis work provides a promising strategy to simplify the drug preparation process, increase drug loading content, reduce systemic toxicity as well as enhance antitumor efficiency.

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Zippered release from polymer-gated carbon nanotubes

Cited by 16 publications

References 61 publications

Biophysical and biological contributions of polyamine-coated carbon nanotubes and bidimensional buckypapers in the delivery of miRNAs to human cells

Biophysical and biological contributions of polyamine-coated carbon nanotubes and bidimensional buckypapers in the delivery of miRNAs to human cells

Enzymatic ‘stripping’ and degradation of PEGylated carbon nanotubes

A facile doxorubicin-dichloroacetate conjugate nanomedicine with high drug loading for safe drug delivery

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