Zinc uptake from ZnSO4 (aq) and Zn-EDTA (aq) and its root-to-shoot transport in soybean plants (Glycine max) probed by time-resolved in vivo X-ray spectroscopy

Nanoparticles of trace elements are easily included in the metabolic cycles of plants. Zinc and molybdenum are important components of enzymes, but even at low concentrations can have phytotoxic effects. Treatment of chufa tubers with microelements increases the viability of tubers, but at certain concentrations of nanoparticles can negatively affect the growth processes at the germination and early vegetative stages. Effect of treatment of tubers with nanoparticle solution of molybdenum, zinc oxide and zinc citrate at a concentration of 20, 40 and 60 ppm on the growth of roots and seedlings was established on 3 rd and 10 th days after emergence. The positive effect of all treatments at a concentration of 20 ppm was found, but higher concentrations caused different effects on root growth. Zinc citrate and molybdenum nanoparticle solution at a concentration of 60 ppm adversely affect the weight of the root, leaves and root length. Pre-sowing treatment with zinc citrate is indirectly more effective than zinc oxide nanoparticles against root growth at concentrations of 20 and 40 ppm but inhibits it at 60 ppm. Treatments with zinc oxide nanoparticles are more stable regardless of concentration. Short-term effects on root growth processes indicate the prospects of increasing drought resistance and other mechanisms of physiological adaptation.

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“…Reason for the phytotoxicity of zinc citrate may be its greater digestibility (Montanha et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

Effect of Nanoparticle Metal Solutions on Chufa (Cyperus Esculentus L.) Root and Seedling Growth

Mazurenko¹,

Honchar²,

Garbar³

2021

AgricultForest

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“…The worldwide survey of the Zn found out 33% of the area is lacking Zn [31]. For annual biomass production of 10 tonnes ha -1 an estimate of 100-300 g, ha -1 Zn is demanded [48]. All the parameters recorded in wheat namely, no of tillers, plant height, spike length, no of spikelets per spike, no of grains per spike, 1000 grain weight, biological yield, grain yield, and harvesting index, Ca, Mg, Fe, Zn, Cu and protein content are significantly affected [39].…”

Section: Zinc Fertilizersmentioning

confidence: 99%

The First Reported Case of Bullous Lichen Planus/Systemic Lupus Erythematosus Overlap Syndrome

Zhuo¹

2021

BJSTR

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Agriculture is the mainstay between humans and the environment. The existence of arbuscular mycorrhizal fungi (AMF), on the earth, is about 600 million years ago. The application of mycorrhizae as biofertilizer is in increasing trend. Mycorrhizae improve several agricultural practices like better nutrient cycling, improving crop yield, and remediation of toxic heavy metals from soil. There is much variation in arbuscular mycorrhizal association although 80% of the plant species are infected with mycorrhizae. Mycorrhizal dependency (MD) is defined as the degree to which a host plant is dependent on AMF to produce maximum growth or yield at a given level of soil fertility. Plant with high MD need to feed with a higher amount of carbon and lipid to the fungus than a plant with less MD. Mycorrhizae modify, maintain, and create habitat by directly and indirectly regulating biotic and abiotic environments. Mycorrhizae derived photosynthetically formed C for the growth and uptake, tolerance against abiotic stress such as drought, heavy metals, salinity as well as protecting from pathogen attack to host plant and preventing from erosion. Application of ZnO would have been the probable solution of mitigating drought.ZnO treatment decreased the adverse effects of drought stress in plants by enhancing antioxidant enzyme activity and changing physiological parameters. Heat stress affects many processes in a variety of plants as water relations, nutrient uptake, photosynthesis, assimilate partitioning, respiration, growth, and reproduction, and induced oxidative damage. Foliar application of ZnSO4.7H2O effectively alleviated by enhancing Zn concentration, superoxide dismutase activity, chlorophyll content, Fv/Fm ratio, and photosystem II under heat stress. In maize, there is a substantial reduction of germination above 37oC. All the parameters recorded in wheat, namely, no of tillers, plant height, spike length, no of spikelets per spike, no of grains per spike, 1000 grain weight, biological yield, grain yield and harvesting index, Ca, Mg, Fe, Zn, Cu and protein content are significantly affected by the mycorrhizal application. Almost 25% of recommended dose of phosphate fertilizer could be saved as in Niger(Guizotia abyssinica)by inoculating Glomusmosseae. Zinc is a vital micronutrient for many plants but its excess can be calamitous.AMF contributes to plant Zn uptake but their role in the edible portion of the crop has not been studied yet. The mycorrhizal pathway of Zn uptake contributed up to 24.3% of the total above-ground Zn in wheat and up to 12% of that Zn in Barley. The greatest contribution by the mycorrhizal pathway was observed in Barley at the lowest Zn addition and in wheat at the .highest one. Besides the grain yield of bread wheat was increased by AMF.

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“…In this scenario, X-ray fluorescence spectroscopy (XRF) has been widely employed to explore the elemental composition and spatial distribution in a myriad of botanical materials (van der Ent et al 2018; Gomes et al 2019; Montanha et al 2020a; Lau et al 2020; Romeu et al 2021; Macedo et al 2021). New generations of XRF instrumentation, i.e., faster high energy-resolution detectors (van der Ent et al 2018; Montanha et al 2020b) and portable XRF systems enabled to assess plant specimens under in vivo and in situ conditions (Montanha et al 2020b; Montanha et al 2020c; Mijovilovich et al 2020; Soares et al 2021; Corrêa et al 2021), thereby providing means for tracking ever-changing physiological processes, e.g., the absorption of nutrients and pollutants through roots and leaves, and their movement toward tissues while they are taking place (van der Ent et al 2018; da Cruz et al 2019; Montanha et al 2020b; Montanha et al 2020c).…”

Section: Introductionmentioning

confidence: 99%

“…e ., faster high energy-resolution detectors (van der Ent et al . 2018; Montanha et al . 2020b) and portable XRF systems enabled to assess plant specimens under in vivo and situ conditions (Montanha et al .…”

Section: Introductionmentioning

confidence: 99%

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Physiological responses of plants to in vivo XRF radiation damage: insights from anatomical, elemental, histochemical, and ultrastructural analyses

Montanha

Marques

Santos

et al. 2022

Preprint

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X-ray fluorescence spectroscopy (XRF) is a powerful technique for the in vivo assessment of plant tissues. However, the potential X-ray exposure damages might affect the structure and elemental composition of living plant tissues leading to artefacts on the recorded data. Herein, we exposed soybean (Glycine max (L.) Merrill) leaves to several X-ray doses through a polychromatic benchtop microprobe X-ray fluorescence spectrometer, modulating the photon flux by adjusting either the beam size, focus, or exposure time. The structure and physiological responses of the irradiated plant tissues were investigated through light and transmission electron microscopy (TEM). Histochemical tests were also conducted. Depending on the dose, the X-ray exposure induced decreased K and X-ray scattering intensities, and increased Ca, P, and Mn signals on soybean leaves. Anatomical analysis indicated necrosis of the epidermal and mesophyll cells on the irradiated spots, where TEM images revealed the collapse of cytoplasm and cell-wall breaking. Furthermore, the histochemical analysis detected the production of reactive oxygen species, as well as inhibition of chlorophyll autofluorescence in these areas. Under certain X-ray exposure conditions, e.g., high photon flux and exposure time, XRF measurements may affect the soybean leaves structures and ultrastructure, elemental composition, cellular ultrastructure, and induce programmed cell death. These results shed light on the characterization of the radiation damage, and thus, help to assess the X-ray radiation limits and strategies for in vivo for XRF analysis.

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Zinc uptake from ZnSO4 (aq) and Zn-EDTA (aq) and its root-to-shoot transport in soybean plants (Glycine max) probed by time-resolved in vivo X-ray spectroscopy

Cited by 20 publications

References 34 publications

Effect of Nanoparticle Metal Solutions on Chufa (Cyperus Esculentus L.) Root and Seedling Growth

Effect of Nanoparticle Metal Solutions on Chufa (Cyperus Esculentus L.) Root and Seedling Growth

The First Reported Case of Bullous Lichen Planus/Systemic Lupus Erythematosus Overlap Syndrome

Physiological responses of plants to in vivo XRF radiation damage: insights from anatomical, elemental, histochemical, and ultrastructural analyses

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