Zinc transport mediated by barley ZIP proteins are induced by low pH

Pedas, Pai; Husted, Søren

doi:10.4161/psb.4.9.9375

Cited by 36 publications

(12 citation statements)

References 17 publications

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“…1 ( Glyma.01g207300 ). HD‐ZIP family proteins are believed to be involved in cellular uptake of Zn 2+ in barley and maize ( Zea mays L.) (Mondal et al, 2014; Pedas and Husted, 2009). The phytochelatin gene on chr.…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel Genomic Loci Associated with Soybean Shoot Tissue Macro and Micronutrient Concentrations

Dhanapal

Ray²,

Smith³

et al. 2018

The Plant Genome

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The mineral composition of crop shoot tissues is important for yield formation and nutrient remobilization to seeds. The natural diversity that exists within crop species can be used to investigate mechanisms that define plant mineral composition and to identify important genomic loci for these processes. The objective of this study was to determine shoot mineral nutrient concentrations in genetically diverse soybean [ (L.) Merr.] genotypes and to identify genomic regions associated with concentrations of different nutrients in shoot tissue. The genotypes were grown at two locations in 2 yr and characterized for macronutrient (Ca, Mg, P, K, and S) and micronutrient (B, Cu, Fe, Mn, and Zn) concentrations in shoot tissues. Genome-wide association studies were conducted with 31,748 single nucleotide polymorphisms (SNPs) via a unified mixed model to identify SNPs associated with macro- and micronutrient concentrations. The number of putative loci identified for the macronutrients ranged from 11 for Ca to 20 for K. For the micronutrients, the number ranged from 10 for Mn to 24 for Fe. In addition to colocated loci for multiple nutrients, 22 individual SNPs were associated with more than one nutrient such that 11 different nutrient combinations were encompassed by these SNPs. Ultimately, the putative loci identified in this study will need to be confirmed and are expected to aid in the identification of new sources of variation for use in soybean breeding programs as well as for mechanistic studies aimed at understanding the regulation of mineral nutrient uptake, translocation, and shoot tissue concentrations.

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Section: Discussionmentioning

confidence: 99%

Identification of Novel Genomic Loci Associated with Soybean Shoot Tissue Macro and Micronutrient Concentrations

Dhanapal

Ray²,

Smith³

et al. 2018

The Plant Genome

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“…It is known that the accumulation of zinc into the vacuole by CDF transporters (e.g., ZrcA) is mediated through a Zn 2+ /H + antiporter mechanism and relies on the proton gradient generated by the V-ATPase ( 30 ). In contrast, and although it is not completely known how ZIP proteins transport zinc across the plasma membrane, it seems that it is not dependent on the proton gradient generated by the plasma membrane H + -ATPase but is pH dependent, such that intracellular acidification increases zinc transport, whereas extracellular acidification decreases zinc transport ( 31 – 33 ). In this regard, it is plausible that under the alkaline zinc-limiting conditions provided by sRPMI medium, a low PmaA activity would increase intracellular acidification to favor zinc uptake by ZIP transporters located in the plasma membrane.…”

Section: Discussionmentioning

confidence: 99%

Novel Zinc-Attenuating Compounds as Potent Broad-Spectrum Antifungal Agents with In Vitro and In Vivo Efficacy

Cohrt¹,

Marín

Kjellerup

et al. 2018

Antimicrob Agents Chemother

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An increase in the incidence of rare but hard-to-treat invasive fungal pathogens as well as resistance to the currently available antifungal drugs calls for new broad-spectrum antifungals with a novel mechanism of action. Here we report the identification and characterization of two novel zinc-attenuating compounds, ZAC307 and ZAC989, which exhibit broad-spectrum in vitro antifungal activity and in vivo efficacy in a fungal kidney burden candidiasis model. The compounds were identified serendipitously as part of a drug discovery process aimed at finding novel inhibitors of the fungal plasma membrane proton ATPase Pma1. Based on their structure, we hypothesized that they might act as zinc chelators. Indeed, both fluorescence-based affinity determination and potentiometric assays revealed these compounds, subsequently termed zinc-attenuating compounds (ZACs), to have strong affinity for zinc, and their growth inhibitory effects on Candida albicans and Aspergillus fumigatus could be inactivated by the addition of exogenous zinc to fungal growth media. We determined the ZACs to be fungistatic, with a low propensity for resistance development. Gene expression analysis suggested that the ZACs interfere negatively with the expression of genes encoding the major components of the A. fumigatus zinc uptake system, thus supporting perturbance of zinc homeostasis as the likely mode of action. With demonstrated in vitro and in vivo antifungal activity, low propensity for resistance development, and a novel mode of action, the ZACs represent a promising new class of antifungal compounds, and their advancement in a drug development program is therefore warranted.

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“…GmZIP1 is expressed specifically in nodules, but not in roots, stems, or leaves, and the protein is localized to the peribacteroid membrane, suggesting a role in symbiosis. In barley, Zn transporters are induced by low pH (Pedas and Husted 2009).…”

Section: Zip Transportersmentioning

confidence: 99%

Zn Uptake and Translocation in Rice Plants

Ishimaru

Bashir

Nishizawa

2011

Rice

157

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Zinc (Zn) is an essential micronutrient with numerous cellular functions in plants, and its deficiency represents one of the most serious problems in human nutrition worldwide. Zn deficiency causes a decrease in plant growth and yield. On the other hand, Zn could be toxic if excess amounts are accumulated. Therefore, the uptake and transport of Zn must be strictly regulated. In this review, the dominant fluxes of Zn in soil-root-shoot translocation in rice plants (Oryza sativa) are described, including Zn uptake from soils in the form of Zn 2+ and Zn-DMA at the root surface, and Zn translocation to shoots. Knowledge of these fluxes could be helpful to formulate genetic and physiologic strategies to address the widespread problem of Zn-limited crop growth.

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Zinc transport mediated by barley ZIP proteins are induced by low pH

Cited by 36 publications

References 17 publications

Identification of Novel Genomic Loci Associated with Soybean Shoot Tissue Macro and Micronutrient Concentrations

Identification of Novel Genomic Loci Associated with Soybean Shoot Tissue Macro and Micronutrient Concentrations

Novel Zinc-Attenuating Compounds as Potent Broad-Spectrum Antifungal Agents with In Vitro and In Vivo Efficacy

Zn Uptake and Translocation in Rice Plants

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