Zinc supplementation prior to heat shock enhances HSP70 synthesis through HSF1 phosphorylation at serine 326 in human peripheral mononuclear cells

Peng-Winkler, Yifan; Büttgenbach, Anna; Rink, Lothar; Weßels, Inga

doi:10.1039/d2fo01406h

Cited by 1 publication

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References 57 publications

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“…HSF1 promotes the expression of Hsp72 under heat stimulation, which could indicate the occurrence of heat stress to a certain extent [ 36 ]. This research was designed to explore the changes of JNK pathway-related protein expression by WB, to verify the expression changes again through IF, and to analyze the distribution characteristics of the proteins.…”

Section: Discussionmentioning

confidence: 99%

Physiological and Psychological Stress of Microwave Radiation-Induced Cardiac Injury in Rats

Yin

et al. 2023

IJMS

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Electromagnetic waves are widely used in both military and civilian fields, which could cause long-term and high-power exposure to certain populations and may pose a health hazard. The aim of this study was to simulate the long-term and high-power working environment of workers using special electromagnetic radiation occupations to clarify the radiation-induced stress response and cardiac damage and thus gain insights into the mechanisms of injuries caused by electromagnetic radiation. In this study, the combination of microwave and stress was an innovative point, aiming to broaden the research direction with regard to the effect and mechanism of cardiac injury caused by radiation. The myocardial structure was observed by optical and transmission electron microscope, mitochondrial function was detected by flow cytometry, oxidative-stress markers were detected by microplate reader, serum stress hormone was detected by radioimmunoassay, and heart rate variability (HRV) was analyzed by multichannel-physiological recorder. The rats were weighed and subjected to an open field experiment. Western blot (WB) and immunofluorescence (IF) were used to detect the expressions and distributions of JNK (c-Jun N-terminal kinase), p-JNK (phosphorylated c-Jun N-terminal kinase), HSF1 (heat shock factor), and NFATc4 (nuclear factor of activated T-cell 4). This study found that radiation could lead to the disorganization, fragmentation, and dissolution of myocardial fibers, severe mitochondrial cavitation, mitochondrial dysfunction, oxidative-stress injury in myocardium, increase to stress hormone in serum, significant changes in HRV, and a slow gain in weight. The open field experiment indicated that the rats experienced anxiety and depression and had decreased exercise capacity after radiation. The expressions of JNK, p-JNK, HSF1, and NFATc4 in myocardial tissue were all increased. The above results suggested that 30 mW/cm2 of S-band microwave radiation for 35 min could cause both physiological and psychological stress damage in rats; the damage was related to the activation of the JNK pathway, which provided new ideas for research on protection from radiation.

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Section: Discussionmentioning

confidence: 99%

Physiological and Psychological Stress of Microwave Radiation-Induced Cardiac Injury in Rats

Yin

et al. 2023

IJMS

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Zinc supplementation prior to heat shock enhances HSP70 synthesis through HSF1 phosphorylation at serine 326 in human peripheral mononuclear cells

Abstract: Zinc supplementation prior to heat shock increases HSP70 (Heat shock protein 70) expression, which has cytoprotective effects in tissue cells during inflammation. Effects of zinc deficiency in this regard are...

Cited by 1 publication

References 57 publications

Physiological and Psychological Stress of Microwave Radiation-Induced Cardiac Injury in Rats

Physiological and Psychological Stress of Microwave Radiation-Induced Cardiac Injury in Rats

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