Zinc Status Alters Growth and Oxidative Stress Responses in Rat Hepatoma Cells

Sankavaram, Kavitha; Chong, Leelyn; Bruno, Richard S.; Freake, Hedley C.

doi:10.1080/01635581.2014.851713

Cited by 6 publications

(5 citation statements)

References 53 publications

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“…A current leading hypothesis relating to the development of PE is increased placental oxidative stress [114]. Zinc itself has antioxidant capabilities and is an integral structural component of superoxide dismutase, a first line defense antioxidant [115] which has reduced activity in cell lines, animal models and human studies of zinc deficiency [116,117,118,119,120]. Hence, it is possible in pregnancies complicated by PE, that low maternal zinc concentration (˂562.1 µg/L) may reduce the potential to combat rises in free radical production and increase the severity of the complication.…”

Section: Discussionmentioning

confidence: 99%

Association between Maternal Zinc Status, Dietary Zinc Intake and Pregnancy Complications: A Systematic Review

et al. 2016

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Adequate zinc stores in the body are extremely important during periods of accelerated growth. However, zinc deficiency is common in developing countries and low maternal circulating zinc concentrations have previously been associated with pregnancy complications. We reviewed current literature assessing circulating zinc and dietary zinc intake during pregnancy and the associations with preeclampsia (PE); spontaneous preterm birth (sPTB); low birthweight (LBW); and gestational diabetes (GDM). Searches of MEDLINE; CINAHL and Scopus databases identified 639 articles and 64 studies were reviewed. In 10 out of 16 studies a difference was reported with respect to circulating zinc between women who gave birth to a LBW infant (≤2500 g) and those who gave birth to an infant of adequate weight (>2500 g), particularly in populations where inadequate zinc intake is prevalent. In 16 of our 33 studies an association was found between hypertensive disorders of pregnancy and circulating zinc; particularly in women with severe PE (blood pressure ≥160/110 mmHg). No association between maternal zinc status and sPTB or GDM was seen; however; direct comparisons between the studies was difficult. Furthermore; only a small number of studies were based on women from populations where there is a high risk of zinc deficiency. Therefore; the link between maternal zinc status and pregnancy success in these populations cannot be established. Future studies should focus on those vulnerable to zinc deficiency and include dietary zinc intake as a measure of zinc status.

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Section: Discussionmentioning

confidence: 99%

Association between Maternal Zinc Status, Dietary Zinc Intake and Pregnancy Complications: A Systematic Review

et al. 2016

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“… 42 Previous studies showed that zinc-induced apoptosis occurs from 50 μM and higher, but zinc-induced ROS generation is observed at 100 μM doses. 14 , 17 , 25 , 43 , 44 Although, we have not counted intracellular free zinc concentrations after the drug and zinc exposures, in a published study, maximum intracellular zinc concentrations have been obtained at 50 μM ZnSO 4 treatment, and finally decreased to initial level when the concentration of ZnSO 4 exceeded 100 μM. 17 In addition, the cell growth inhibition and apoptosis were evaluated 24 hours after the treatment.…”

Section: Discussionmentioning

confidence: 99%

Zinc supplementation induces apoptosis and enhances antitumor efficacy of docetaxel in non-small-cell lung cancer

Koçdor¹,

Ateş²,

Aydın³

et al. 2015

DDDT

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BackgroundExposure to exogenous zinc results in increased apoptosis, growth inhibition, and altered oxidative stress in cancer cells. Previous studies also suggested that zinc sensitizes some cancer cells to cytotoxic agents depending on the p53 status. Therefore, zinc supplementation may show anticancer efficacy solely and may increase docetaxel-induced cytotoxicity in non-small-cell lung cancer cells.MethodsHere, we report the effects of several concentrations of zinc combined with docetaxel on p53-wild-type (A549) and p53-null (H1299) cells. We evaluated cellular viability, apoptosis, and cell cycle progression as well as oxidative stress parameters, including superoxide dismutase, glutathione peroxidase, and malondialdehyde levels.ResultsZinc reduced the viability of A549 cells and increased the apoptotic response in both cell lines in a dose-dependent manner. Zinc also amplified the docetaxel effects and reduced its inhibitory concentration 50 (IC50) values. The superoxide dismutase levels increased in all treatment groups; however, glutathione peroxidase was slightly increased in the combination treatments. Zinc also caused malondialdehyde elevations at 50 μM and 100 μM.ConclusionZinc has anticancer efficacy against non-small-cell lung cancer cells in the presence of functionally active p53 and enhances docetaxel efficacy in both p53-wild-type and p53-deficient cancer cells.

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“…Zinc was shown to be a critical regulator of cell growth and death. Previous findings indicate that the mechanistic actions of zinc are shown through change in caspase enzyme activities, as well as the direct alteration of apoptotic regulator expression (16)(17)(18)(19). As shown in Fig.…”

Section: Knockdown Of Liv1 Protects Cells From Tsa-induced Apoptosis mentioning

confidence: 72%

Effect of LIV1 on the sensitivity of ovarian cancer cells to trichostatin A

Duan

Liu

et al. 2014

Oncology Reports

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In a previous study, we used a functional gene screen approach to identify the key genes responsible for the tumor-selective action of trichostatin A (TSA), of which LIV1, a novel zinc transporter, was isolated by its marked ability to confer resistance against TSA-induced apoptosis. The aim of the present study was to investigate the effect of LIV1 expression on the sensitivity of ovarian cancer cells to TSA. We tested the induction of LIV1 in ovarian cancer cells and clinical samples after TSA treatment by real-time PCR and western blot analysis. We investigated the effect of LIV1 expression on the sensitivity of ovarian cancer cells to TSA by MTT assay, flow cytometry and colony forming assays. Finally, we analyzed the mechanism of LIV1 in ovarian cancer cells by western blot analysis. We found that the levels of LIV1 mRNA and protein were significantly upregulated after TSA treatment. The viability and colony forming rates of the ovarian cancer cells transfected with AS-LIV1 (pCEP4 carrying antisense LIV1 cDNA) were obviously higher than the rates of the control as detected by MTT and colony forming assays, which could be reversed by FL-LIV1 (pCEP4 carrying full-length LIV1 cDNA). The apoptotic rate of the AS-LIV1 cells was markedly lower than the rate of the control as determined FACS. Using western blot analysis, we demostrated that the inhibition of TSA-induced apoptosis by knockdown of LIV1 might be associated with decreased endogenous levels of Bcl-2, enhanced levels of Bax and cleavage of procaspase-3. The present study suggests that the drug resistance of ovarian cancer cells to TSA may be related to expression of the LIV1 gene, and targeting LIV1 could be exploited as a novel strategy to more effectively kill ovarian cancer cells.

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Zinc Status Alters Growth and Oxidative Stress Responses in Rat Hepatoma Cells

Cited by 6 publications

References 53 publications

Association between Maternal Zinc Status, Dietary Zinc Intake and Pregnancy Complications: A Systematic Review

Association between Maternal Zinc Status, Dietary Zinc Intake and Pregnancy Complications: A Systematic Review

Zinc supplementation induces apoptosis and enhances antitumor efficacy of docetaxel in non-small-cell lung cancer

Effect of LIV1 on the sensitivity of ovarian cancer cells to trichostatin A

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Zinc Status Alters Growth and Oxidative Stress Responses in Rat Hepatoma Cells

Cited by 6 publications

References 53 publications

Association between Maternal Zinc Status, Dietary Zinc Intake and Pregnancy Complications: A Systematic Review

Association between Maternal Zinc Status, Dietary Zinc Intake and Pregnancy Complications: A Systematic Review

Zinc supplementation induces apoptosis and&nbsp;enhances antitumor efficacy of docetaxel in&nbsp;non-small-cell lung cancer

Effect of LIV1 on the sensitivity of ovarian cancer cells to trichostatin A

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Zinc supplementation induces apoptosis and enhances antitumor efficacy of docetaxel in non-small-cell lung cancer