Zinc sensing for cellular application

Kikuchi, Kazuya; Komatsu, Kazuya; Nagano, Tetsuo

doi:10.1016/j.cbpa.2004.02.007

Cited by 308 publications

(160 citation statements)

References 49 publications

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“…The development of highly sensitive, selective, and cell membrane permeable probes that exhibit a visible "turn-on" fluorescent emission in aqueous media is very much necessary for fluorescence imaging of metal ions in living cells. Till date a number of fluorescence probes has been reported for imaging various intracellular heavy metal ions such as Zn 2 þ , Cd 2 þ , Cu þ , Pb 2 þ , Fe 3 þ , Hg 2 þ and Cu 2 þ [14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

A new rhodamine B based fluorometric chemodosimeter for Cu2+ ion in aqueous and cellular media

Kempahanumakkagaari

Ramakrishnappa

Malingappa

2014

Journal of Luminescence

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a b s t r a c tA simple, sensitive and selective fluorescent chemo dosimeter rhodamine B phenyl hydrazide (RBPH) for Cu 2 þ was proposed. This probe is non fluorescent and colorless but exhibits fluorescent enhancement at 580 nm and displayed color change from colorless to pink for Cu 2 þ in the pH range 1-6. Fluorescence microscope experimental results reveals that this chemo sensor is cell permeable and can be used for fluorescence imaging of Cu 2 þ ions in living cells. This probe can detect Cu 2 þ with good linear relationships from 10 to 100 nM with r ¼0.99971 then limit of detection was found to be 0.015 nM with 7 0.91% RSD at 10 nM concentrations.

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Section: Introductionmentioning

confidence: 99%

A new rhodamine B based fluorometric chemodosimeter for Cu2+ ion in aqueous and cellular media

Kempahanumakkagaari

Ramakrishnappa

Malingappa

2014

Journal of Luminescence

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“…While a great deal is known about the biochemistry of Zn(II) in relation to metalloproteins, far less is understood about the specific mechanisms of its cellular physiology and distribution because it is tightly bound to zincbinding ligands (4). To understand the specific functions of Zn(II), research has focused on the development of Zn(II) fluorescent probes (5,6). These Zn(II) probes are changing our understanding of the biological function of this important ion in cell and tissue culture experiments.…”

mentioning

confidence: 99%

The synthesis and in vitro testing of a zinc-activated MRI contrast agent

Major

Parigi

Luchinat

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

177

164

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Zinc(II) plays a vital role in normal cellular function as an essential component of numerous enzymes, transcription factors, and synaptic vesicles. While zinc can be linked to a variety of physiological processes, the mechanisms of its cellular actions are less discernible. Here, we have synthesized and tested a Zn(II)-activated magnetic resonance imaging (MRI) contrast agent in which the coordination geometry of the complex rearranges upon binding of Zn(II). In the absence of Zn(II) water is restricted from binding to a chelated Gd(III) ion by coordinating acetate arms resulting in a low relaxivity of 2.33 mM ؊1 ⅐s ؊1 at 60 MHz. Upon addition of Zn(II) the relaxivity of the Gd(III)-Zn(II) complex increases to 5.07 mM ؊1 ⅐s ؊1 and is consistent with one water molecule bound to Gd(III). These results were confirmed by nuclear magnetic relaxation dispersion analysis. There was no observed change in relaxivity of the Gd(III) complex when physiologically competing cations Ca(II) and Mg(II) were added. A competitive binding assay gave a dissociation constant of 2.38 ؋ 10 ؊4 M for the Gd(III)-Zn(II) complex. In vitro magnetic resonance images confirm that Zn(II) concentrations as low as 100 M can be detected by using this contrast agent. biological molecular imaging ͉ zinc sensing ͉ gadolinium Z inc(II) plays a critical role in cellular physiology and is involved in structural stability, catalytic activity, and signal transduction processes (1-3). While a great deal is known about the biochemistry of Zn(II) in relation to metalloproteins, far less is understood about the specific mechanisms of its cellular physiology and distribution because it is tightly bound to zincbinding ligands (4). To understand the specific functions of Zn(II), research has focused on the development of Zn(II) fluorescent probes (5, 6). These Zn(II) probes are changing our understanding of the biological function of this important ion in cell and tissue culture experiments.Our goal has been to noninvasively image Zn(II) activity in whole organisms, and we have focused on developing Zn(II) magnetic resonance (MR) contrast agents. Unlike light-based microscopy, MRI can provide three-dimensional images without the limitations of light scattering and photobleaching (7,8). MRI takes advantage of the most abundant molecule in biological tissues, water. In the presence of a magnetic field the magnetic moments of the protons in water molecules orient themselves along the magnetic field. An applied radiofrequency pulse inverts the magnetization vector, and reorientation to the original magnetic field direction occurs with a characteristic time constant. This process of realignment characterized by T 1 is called longitudinal or spin-lattice relaxation, and it is the dominant factor in producing contrast in a T 1 -weighted MR image. While intrinsic contrast between organs can be observed by using MRI, resolution and sensitivity improve greatly with the use of contrast agents such as Gd(III) chelates. The efficacy of these complexes to decrease the T 1 o...

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“…Simultaneously, the positive charge of the metal ion repulses that of the imidazolium cation, which makes the π-π stacking interaction more stable. (14) Fig . The metallic complex can emit very strong fluorescence because of the enhanced rigidity and the coplanar molecular structure of L-M-Py. Generally, the fluorescence emission mechanism of a metallic complex is as follows: first, the π-electrons in the conjugation system in the ligand molecule absorb visible light.…”

Section: Fluorescence Property Of Metal Complexesmentioning

confidence: 99%

Imidazolium-Salt-Based Fluorescent Chemosensor for Cu(II), Zn(II) and Co(II)

2008

Sensors and Materials

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A ligand of macrocyclic polyamine with an imidazolium salt group (MCP-ISG) and its Cu(II), Zn(II) and Co(II) metallic complexes were synthesized, and MCP-ISG was used as a chelation-enhanced fluorophore. The complexes of Cu(II), Zn(II) and Co(II) have different intensities of fluorescence response in a solution of pyridine/water (1:1). The fluorescence intensity of Cu(II) complex was enhanced in the solution while those of Co(II) and Zn(II) complexes were weakened markedly, with the Zn(II) complex showing the larger reduction. The interaction and fluorescence emission mechanisms of the ligand with the cations are also disccussed in this paper.

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Zinc sensing for cellular application

Cited by 308 publications

References 49 publications

A new rhodamine B based fluorometric chemodosimeter for Cu2+ ion in aqueous and cellular media

A new rhodamine B based fluorometric chemodosimeter for Cu2+ ion in aqueous and cellular media

The synthesis and in vitro testing of a zinc-activated MRI contrast agent

Imidazolium-Salt-Based Fluorescent Chemosensor for Cu(II), Zn(II) and Co(II)

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