Zinc Phosphide Induced DNA Damage in the Blood Cells of &lt;i&gt;Gallus&lt;/i&gt; sp. using Comet DNA Assay

Adnan, Md.

doi:10.3844/ajabssp.2012.82.87

Cited by 4 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sister chromatid test, chromosome aberrations, comet assay, and micronucleus test are currently the most widely employed methods to detect DNA lesions in ecotoxicology. Sister chromatid test and chromosome aberrations are time consuming, resource intensive, require proliferating cell population [12], and not very effective due to the relatively large number of small chromosomes of many aquatic animal species. Theses disadvantages have stimulated the development and the use of comet assay and micronucleus test as simpler, more sensitive and time efficiency genotoxicity tests.…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity Tests and Their Contributions in Aquatic Environmental Research

Osman¹

2014

JEP

View full text Add to dashboard Cite

As many chemicals with genotoxic potential are emitted to surface water, genotoxicity tests are gaining importance which led to the development of several techniques to detect directly DNA damage. The relevance of detecting the genotoxic risks associated with water pollution was firstly perceived in the late 1970s. Since that time several tests have been developed for evaluating DNA alterations in aquatic animals. These tests rely on the premise that any changes to DNA may have long-lasting and profound consequences. Sister chromatid test, chromosome aberrations, comet assay, and micronucleus test are currently the most widely employed methods to detect DNA lesions in ecotoxicology. Chromosomal aberration and sister chromatid exchanges are time consuming, resource intensive and require proliferating cell population. Hence, Comet assay and Micronucleus test as cost effective and more sensitive test systems have now been introduced for assessing the genotoxicity of chemicals. This review presents a synthesis of the state of the art in the methodologies of comet assay and micronucleus test and their contributions in aquatic environmental research. The text explores the latest knowledge and thinking on these very important approaches for the assessment of environmental health, management, and conservation. The primary concern of the present review is the measurement of genotoxic potential in aquatic organisms under field and laboratory conditions, where effects of chemicals at different levels of biological organization can be examined.

show abstract

Section: Introductionmentioning

confidence: 99%

Genotoxicity Tests and Their Contributions in Aquatic Environmental Research

Osman¹

2014

JEP

View full text Add to dashboard Cite

show abstract

“…Muid et al (2012) has pointed out that the comet assay is considered a suitable and rapid test for DNA-damaging potential in environmental and biomonitoring studies.Research reports indicate that it can be applicable to freshwater and marine fishes and that gill cells are more sensitive than the hematopoietic cells to micronucleus inducing agents which has been reported by most of the researchers (Al-Sabti and Metcalfe, 1995;Cavas et al, 2005;Fontanetti et al, 2010;Palus et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

<i>IN-VIVO</i> EVALUATION OF HEXAVALENT CHROMIUM INDUCED DNA DAMAGE BY ALKALINE COMET ASSAY AND OXIDATIVE STRESS IN <i>CATLA CATLA</i>

Arunachalam

Annamalai

Kuruva

2013

American Journal of Environmental Sciences

View full text Add to dashboard Cite

In the present study, the acute toxicity of Chromium in fingerlings of Catla catla, an Indian major carp, was evaluated with renewal bioassay method. In vivo studies were designed to assess the extent of Micronucleus Assay, Comet Assay under the exposure of common heavy-metal compounds, namely, Chromium Nitrate, using Catla catla (2n = 20), as a test model. The laboratory acclimatized fishes were divided into four groups. Group I served as positive control and the other three as exposed groups for three different time durations of 7, 14 and 21 days and were subjected to uninterrupted sub lethal concentrations (50% of 96 h LC 50 ). The experiments were planned in such a way that fish from all the groups were sacrificed on the same day. The frequencies of micronuclei and bi-nuclei were evaluated comparatively in peripheral erythrocytes. As a result, it was observed that, the fishes and different tissues showed differential sensitivity to the heavy-metal treatment. A significant increase in the frequencies of micronucleated and binucleated cells and percentage increase in DNA tail (p<0.001) through Alkaline Comet Assay were observed after 21 days of exposure to chromium. Our results also showed decrease in enzyme Superoxide Dismutase (SOD) activity and increase in catalase enzyme due to increasing chromium concentration. The bio-concentration factor profiles of Chromium in Catla catla during sub lethal toxicity study was also calculated.

show abstract