Zinc Oxide Nanoparticles Inhibition of Initial Adhesion and ALS1 and ALS3 Gene Expression in Candida albicans Strains from Urinary Tract Infections

Hosseini, Seyedeh Sedigheh; Ghaemi, Ezzatollah; Noroozi, Azita; Niknejad, Farhad

doi:10.1007/s11046-019-00327-w

Cited by 41 publications

(33 citation statements)

References 22 publications

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“…Similarly, in this study, Fluconazole-resistant strains were shown to increase the expression of SAP. 17,27 We used sub-MIC (3±1.7µg/mL) concentrations of ZnO nanoparticles and Nystatin to inhibit the expression of SAP1-3 genes. The results revealed that both ZnO and Nystatin decreased the SAP1-3 genes expression in all isolates that is in accordance with the findings of Cho et al 10,[28][29][30] This finding shows that ZnO nanoparticles 5±1.3µg/mL and Nystatin 3±1.3µg/mL Figure 5 Comparison of the expression of SAP1-3 of VVC C. albicans isolates with 3±1.7µg/mL concentrations of ZnO-nanoparticles and Nystatin compared to the calibrated gene in the control group (Value of the nonadjacent samples was zero).…”

Section: Discussionmentioning

confidence: 99%

Antifungal Activity of ZnO Nanoparticles and Nystatin and Downregulation of SAP1-3 Genes Expression in Fluconazole-Resistant Candida albicans Isolates from Vulvovaginal Candidiasis

Hosseini

Joshaghani

Shokohi

et al. 2020

IDR

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Purpose: Antifungal resistance and virulence properties of Candida albicans (C. albicans) are growing health problems worldwide. The present study aims to investigate the effect of Zinc Oxide (ZnO) nanoparticles and Nystatin on SAP1-3 genes expression in C. albicans isolates of females with Vulvovaginal Candidiasis (VVC) isolated from Sayad Shirazi Obstetrics and Gynecology Hospital in Northeastern Iran during 2017-2018. Patients and Methods: In this descriptive-analytic study, vaginal samples were collected from 280 VVC women. 196 (70%) of C. albicans isolates were identified by phenotypic and ITS genotypic methods. Susceptibility to Fluconazole C. albicans isolates was determined by the disk diffusion method. Detection of ERG11 gene was done by RT-PCR technique. Results: It was revealed that PCR amplified the ERG11 gene in all of the Fluconazole-resistant isolates. Real-time PCR was used to survey the effects of 3±1.7µg/mL concentrations of ZnO nanoparticles and Nystatin on expression of SAP1-3 genes before and after treatment. 186 (95%) susceptible C. albicans and 10 (5%) Fluconazole-resistant C. albicans isolates from VVC were exposed to sub-minimum inhibitory concentrations (Sub-MIC) of ZnO-np (range=0.02-12 μg/ mL). Sub-MIC concentration was used for each strain, which reduced the expression of SAP1-3 genes to 1.8 MIC in the vaginal swabs. The observed reduction in gene expression was significant for both ZnO nanoparticles and Nystatin (P=0.01 and P=0.07, respectively). Conclusion: ZnO as antifungal agent can well reduce the growth and gene expression of SAP1-3 in the pathogenesis of VVC.

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Section: Discussionmentioning

confidence: 99%

Antifungal Activity of ZnO Nanoparticles and Nystatin and Downregulation of SAP1-3 Genes Expression in Fluconazole-Resistant Candida albicans Isolates from Vulvovaginal Candidiasis

Hosseini

Joshaghani

Shokohi

et al. 2020

IDR

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“…Ct indicates the average threshold period of the genes obtained in three independent experiments ( Escribano et al, 2017 ). Data are presented as mRNA transcripts (arbitrary units) relative to ACT1 ( Hosseini et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

ALS3 Expression as an Indicator for Candida albicans Biofilm Formation and Drug Resistance

et al. 2021

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Resistance caused by the formation of the Candida albicans (C. albicans) biofilm is one of the main reasons for antifungal therapy failure. Thus, it is important to find indicators that predict C. albicans biofilm formation to provide evidence for the early prevention and treatment of the C. albicans biofilms. In this study, C. albicans samples were selected from C. albicans septicemia that were sensitive to common antifungal agents. It was found that the agglutinin-like sequence 3 (ALS3) gene was differentially expressed in free, antifungal, drug-sensitive C. albicans. The average ALS3 gene expression was higher in the C. albicans strains with biofilm formation than that in the C. albicans strains without biofilm formation. Then, it was further confirmed that the rate of biofilm formation was higher in the high ALS3 gene expression group than that in the low ALS3 gene expression group. It was found that C. albicans with biofilm formation was more resistant to fluconazole, voriconazole, and itraconazole. However, it maintained its sensitivity to caspofungin and micafungin in vitro and in mice. Further experiments regarding the prevention of C. albicans biofilm formation were performed in mice, in which only caspofungin and micafungin prevented C. albicans biofilm formation. These results suggest that the expression level of ALS3 in C. albicans may be used as an indicator to determine whether C. albicans will form biofilms. The results also show that the biofilm formation of C. albicans remained sensitive to caspofungin and micafungin, which may help to guide the selection of clinical antifungal agents for prevention and therapy.

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“…For that, they treated C. albicans strains with a sub-MIC concentration of ZnO NPs. Results indicated that, after treatment with the sub-MIC concentration of ZnO NPs, the expression of ALS1 and ALS3 decreased significantly, suggesting ZnO NPs as an effective antibiofilm agent (Hosseini et al, 2019). In another investigation, Hosseini et al studied the influence of ZnO NPs on C. albicans biofilm derived from urinary-catheters isolated strains, some of which were fluconazole resistant.…”

Section: Antibacterialmentioning

confidence: 99%

“…Nowadays, inorganic NPs are widely considered as catheters coating, among which AgNPs are already being used to prevent catheter bacterial colonization, while other inorganic materials are currently under investigation. All reported inorganic compounds-based NPs as well as organic NPs, show a tendency to be include in urinary tract devices to prevent biofilm formation and reduce CAUTIs (Meenakumari et al, 2000;Ulett et al, 2007;Syed et al, 2009;Syed et al, 2010;Evliyaoglu et al, 2011;Agarwala et al, 2014;Prabha et al, 2014;Ahmed et al, 2016;Shafreen et al, 2017;Shalom et al, 2017;Bhargava et al, 2018;Hosseini et al, 2018;Rajivgandhi et al, 2018;Divya et al, 2019;Iribarnegaray et al, 2019;Hosseini et al, 2019;Kanugala et al, 2019). Furthermore, some investigations have exposed that their synthetized NPs could have the potential to be used either as a catheter coating or as a systemic administration drug.…”

Section: Other Inorganic Nanoparticlesmentioning

confidence: 99%

Nanoparticles as Potential Novel Therapies for Urinary Tract Infections

Sánchez

Navarro

Catalán-Figueroa

et al. 2021

Front. Cell. Infect. Microbiol.

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Urinary tract infection (UTI) is one of the most common reasons for antibiotic treatment. Nevertheless, uropathogens are steadily becoming resistant to currently available therapies. In this context, nanotechnology emerges as an innovative and promising approach among diverse strategies currently under development. In this review we deeply discuss different nanoparticles (NPs) used in UTI treatment, including organic NPs, nanodiamonds, chemical and green synthesized inorganic NPs, and NPs made of composite materials. In addition, we compare the effects of different NPs against uropathogens in vivo and in vitro and discuss their potential impact the in the near future.

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Zinc Oxide Nanoparticles Inhibition of Initial Adhesion and ALS1 and ALS3 Gene Expression in Candida albicans Strains from Urinary Tract Infections

Cited by 41 publications

References 22 publications

<p>Antifungal Activity of ZnO Nanoparticles and Nystatin and Downregulation of SAP1-3 Genes Expression in Fluconazole-Resistant <em>Candida albicans</em> Isolates from Vulvovaginal Candidiasis</p>

<p>Antifungal Activity of ZnO Nanoparticles and Nystatin and Downregulation of SAP1-3 Genes Expression in Fluconazole-Resistant <em>Candida albicans</em> Isolates from Vulvovaginal Candidiasis</p>

ALS3 Expression as an Indicator for Candida albicans Biofilm Formation and Drug Resistance

Nanoparticles as Potential Novel Therapies for Urinary Tract Infections

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