Zinc oxide nanoparticles inhibit murine photoreceptor‐derived cell proliferation and migration <i>via</i> reducing TGF‐β and MMP‐9 expression <i>in vitro</i>

Guo, Da Dong; Li, Qing Ning; Li, Chun Min; Bi, Hong

doi:10.1111/cpr.12163

Cited by 29 publications

(14 citation statements)

References 52 publications

(59 reference statements)

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“…ZnONPs increased lactate dehydrogenase release in medium, aggravate ROS level within cells, collapse mitochondrial membrane potential, block delayed rectifier potassium current, and attenuate expressions of Na + /K + -ATPase at mRNA and protein levels and its activity (19,20). ZnONPs also showed adverse effects on murine photoreceptor cells, via reducing TGF-β and MMP-9 expression in vitro (21). Regarding selective toxicity of ZnONPs to SIRC cells, it seems that there are some different proteins or signals with roles in cytotoxic pathway in SIRC cells.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Cytotoxicity of Zinc Oxide Nanoparticles in Cultured Statens Seruminstitut Rabbit Cornea Cells

Lee

Park

2019

ToxicolRes

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The possibility of eye exposure for workers participating in manufacturing of nanoparticles or consumers using products containing nanoparticles has been reported, but toxicity studies on the eye are scarce. In this study, cytotoxicity of five nanoparticles including silver, ceria, silica, titanium and zinc were tested using Statens Seruminstitut Rabbit Cornea (SIRC) cells. When cells were treated with nanoparticles with concentrations of 1–100 μg/mL for 24 hr, zinc oxide nanoparticles showed higher toxicity to cornea cells. LC 50 of zinc oxide nanoparticles was less than 25 μg/mL but those of other nanoparticles could not be calculated in this test, which means more than 100 μg/mL. Generation of reactive oxygen species was observed, and expression of apoptosis related biomarkers including Bax and Bcl-2 were changed after treatment of zinc oxide nanoparticles, while no other significant toxicity- related changes were observed in cornea cells treated with Ag, CeO 2 , SiO 2 and TiO 2 nanoparticles.

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Section: Discussionmentioning

confidence: 99%

In Vitro Cytotoxicity of Zinc Oxide Nanoparticles in Cultured Statens Seruminstitut Rabbit Cornea Cells

Lee

Park

2019

ToxicolRes

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“…An increase in NPs concentration in cells contributes its attachment on cell surface followed by its uptake in cytoplasm resulting in cell death. ROS are produced in the cells in response to ZnO NPs exposure leading to free radical formation which reacts with the cell organelles to cause enzymatic changes, facilitates cellular content disorganization ultimately resulting in apoptosis [76,77]. 7b) and DU145 ( Figure 7f) cells compared to treatments and more when compared with negative controls (Figure 7a,e).…”

Section: Dapi Stainingmentioning

confidence: 99%

Crotalaria verrucosa Leaf Extract Mediated Synthesis of Zinc Oxide Nanoparticles: Assessment of Antimicrobial and Anticancer Activity

et al. 2020

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In this work, we present an ecofriendly, non-hazardous, green synthesis of zinc oxide nanoparticles (ZnO NPs) by leaf extract of Crotalaria verrucosa (C. verrucosa). Total phenolic content, total flavonoid and total protein contents of C. verrucosa were determined. Further, synthesized ZnO NPs was characterized by UV–visible spectroscopy (UV-vis), X-ray diffractometer (XRD), Fourier transform infra-red (FTIR) Spectra, transmission electron microscope (TEM), and Dynamic light scattering (DLS) analysis. UV-vis shows peak at 375 nm which is unique to ZnO NPs. XRD analysis demonstrates the hexagonal phase structures of ZnO NPs. FTIR spectra demonstrates the molecules and bondings associated with the synthesized ZnO NPs and assures the role of phytochemical compounds of C. verrucosa in reduction and capping of ZnO NPs. TEM image exhibits that the prepared ZnO NPs is hexagonal shaped and in size ranged between 16 to 38 nm which is confirmed by DLS. Thermo-gravimetric analysis (TGA) was performed to determine the thermal stability of biosynthesized nanoparticles during calcination. The prepared ZnO NPs showed significant antibacterial potentiality against Gram-positive (S. aureus) and Gram-negative (Proteus vulgaris, Klebsiella pneumoniae, and Escherichia coli) pathogenic bacteria and SEM image shows the generalized mechanism of action in bacterial cell after NPs internalization. In addition, NPs are also found to be effective against the studied cancer cell lines for which cytotoxicity was assessed using MTT assay and results demonstrate highest growth of inhibition at the concentration of 100 µg/mL with IC50 value at 7.07 µg/mL for HeLa and 6.30 µg/mL for DU145 cell lines, in contrast to positive control (C. verrucosa leaf extract) with IC50 of 22.30 µg/mL on HeLa cells and 15.72 µg/mL on DU145 cells. Also, DAPI staining was performed in order to determine the effect on nuclear material due to ZnO NPs treatment in the studied cell lines taking leaf extract as positive control and untreated negative control for comparison. Cell migration assay was evaluated to determine the direct influence of NPs on metastasis that is potential suppression capacity of NPs to tumor cell migration. Outcome of the synthesized ZnO NPs using C. verrucosa shows antimicrobial activity against studied microbes, also cytotoxicity, apoptotic mediated DNA damage and antiproliferative potentiality in the studied carcinoma cells and hence, can be further used in biomedical, pharmaceutical and food processing industries as an effective antimicrobial and anti-cancerous agent.

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“…It is also reported that ZnO nanoparticles induce death in proliferating cells. All these properties are very important for an IOL. Thus, the main objective of the present study is to prepare hydrophobic polymer‐ZnO nanocomposite films and study the effect of added ZnO on film properties that are important in manufacture and function of IOLs.…”

Section: Resultsmentioning

confidence: 99%

“…Zinc oxide (ZnO) nanoparticles are known to be selective killers of proliferating cells. [16][17][18][19] It was also shown that ZnO nanoparticles and UV radiation have synergistic inhibitory effect on human lens epithelial cells. 20,21 Thus, nanocomposites of ZnO with hydrophobic polymers offer excellent scope for preparation of IOLs that resist cellular adhesion, and the same has been investigated in the present study.…”

Section: Introductionmentioning

confidence: 99%

“Cell adhesion resistant, UV curable, polymer zinc oxide nanocomposite materials for intraocular lens application”

Hussain

Shailaja

Tammishetti

et al. 2017

Polymers for Advanced Techs

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Foldable acrylic lenses are used for surgical replacement of diseased intraocular lens in treatment of cataract. One of the main postsurgical complications of this treatment method is postcapsular opacification caused by proliferating epithelial cells. The most common approach taken by research community to address this issue has been modification of lens surface with hydrophobic or hydrophilic moieties to manipulate surface cell interaction. In the present study, inherently cell repellent photopolymer resins are presented. Taking advantage of zinc oxide antiproliferative properties, its nanocomposite resins were made and investigated. Hydrophobically modified zinc oxide nanoparticles and poly(phenylethylacrylate‐co‐phenylethyl methacrylate) were made, and their nanocomposite films were prepared by UV polymerization. Films made with 5 wt% ZnO nanoparticles in them resisted fibroblast attachment substantially. In addition, these films filtered harmful UV light and showed other necessary properties like visible light transparency, glass transition temperatures, mechanical strength, and biocompatibility necessary for making intraocular lenses.

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Zinc oxide nanoparticles inhibit murine photoreceptor‐derived cell proliferation and migration via reducing TGF‐β and MMP‐9 expression in vitro

Cited by 29 publications

References 52 publications

In Vitro Cytotoxicity of Zinc Oxide Nanoparticles in Cultured Statens Seruminstitut Rabbit Cornea Cells

In Vitro Cytotoxicity of Zinc Oxide Nanoparticles in Cultured Statens Seruminstitut Rabbit Cornea Cells

Crotalaria verrucosa Leaf Extract Mediated Synthesis of Zinc Oxide Nanoparticles: Assessment of Antimicrobial and Anticancer Activity

“Cell adhesion resistant, UV curable, polymer zinc oxide nanocomposite materials for intraocular lens application”

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