Zinc mapping in bone tissues by histochemistry and synchrotron radiation–induced x-ray emission: correlation with the distribution of alkaline phosphatase

Gómez, Santiago; Rizzo, Roberto; Pozzi-Mucelli, Massimo; Bonucci, E.; Vittur, Franco

doi:10.1016/s8756-3282(99)00102-7

Cited by 75 publications

(68 citation statements)

References 39 publications

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“…Zn is a normal trace component of periodontium [38] and bone, and is presumed to have a role in bone formation. It has been found associated with osteons and Haversian canals [36]. The King William Island (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Individual metals are displayed using a color scale based on the maximum concentration of the metal; the scale is not normalized in such a way as to allow direct comparison of the concentrations of different metals. While it has been suggested that a detailed study of the Haversion canals in bone may provide a chronology of Pb accumulation [36], the existing literature [16][17][18][19][20][21][22][23] on the kinetics of Pb/bone exchange make it clear that the Pb distribution patterns within bone will provide a realistic chronology of uptake. The significance of the distribution of other metals must be uncertain, though the Beechey Island materials (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Pb distribution in bones from the Franklin expedition: synchrotron X-ray fluorescence and laser ablation/mass spectroscopy

et al. 2013

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Synchrotron micro-X-ray Fluorescence has been used to map the metal distribution in selected bone fragments representative of remains associated with the Franklin expedition. In addition, laser ablation mass spectroscopy using a 25 µm diameter circular spot was employed to compare the Pb isotope distributions in small regions within the bone fragments. The X-ray Fluorescence mapping shows Pb to be widely distributed in the bone while the Pb isotope ratios obtained by laser ablation within small areas representative of bone with different Pb exchange rates do not show statistically significant differences. These results are inconsistent with the hypothesis that faulty solder seals in tinned meat were the principle source of Pb in the remains of the expedition personnel.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Pb distribution in bones from the Franklin expedition: synchrotron X-ray fluorescence and laser ablation/mass spectroscopy

et al. 2013

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“…2e, f) 6 . Zinc is an excellent marker for bone growth and remodeling as it is known to deposit between zones of bone calcification 6,9 . The heterogeneous accumulation of both tungsten and zinc at the inner and outer edges of the cortical bone tissue is likely Table 1 for intensity values connected to the changing width, and thus increased remodeling activity, of the long bone in these young, growing mice.…”

Section: Resultsmentioning

confidence: 99%

Accumulation of persistent tungsten in bone as in situ generated polytungstate

et al. 2018

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Tungsten accumulates in bone but is neither labile nor inert once absorbed. Tungsten's relatively high cytosolic solubility and availability are problematic given its association with childhood lymphocytic leukemia. In light of tungsten's technological prevalence, and the increased concern of regulatory agencies, here we characterize the chemical form and localization in mice exposed to tungsten through drinking water. Using X-ray fluorescence spectroscopy, we report accumulation of tungsten in bone tissue with some sites having~10-fold greater intensities than background levels. The long bone tissue studied includes cortical, cancellous and bone marrow. Persistence of tungsten in cortical bone tissue following removal of the source indicates that it is retained in an insoluble form. The X-ray absorption near-edge structure spectra for tungsten in these tissues indicate that it is no longer in the originally administered form, orthotungstate, but rather resembles the heteropolytungsate species, phosphotungstate.

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“…A number of studies have identified zinc in bone and dental tissues [19,22,[28][29][30][31][32][33][34][35]. Overall, cementum is zinc-enriched (407 ppm) compared with dentine and bone [34].…”

Section: Trace Elements In Cementummentioning

confidence: 99%

“…Overall, cementum is zinc-enriched (407 ppm) compared with dentine and bone [34]. Slow forming peritubular dentine is also zinc-rich [36,37] as is the forming mineral front in bone [32] which has prompted suggestions that Zn is elevated where mineralisation is active but also that high levels at the mineralising front itself may only be transient [37]. On this basis it has been predicted that Zn will be strongly concentrated at the cementum surface in all vertebrates with apatitic teeth [30].…”

Section: Trace Elements In Cementummentioning

confidence: 99%

Incremental distribution of strontium and zinc in great ape and fossil hominin cementum using synchrotron X-ray fluorescence mapping

Dean

Cabec

Spiers

et al. 2018

J. R. Soc. Interface.

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Cementum and the incremental markings it contains have been widely studied as a means of ageing animals and retrieving information about diet and nutrition. The distribution of trace elements in great ape and fossil hominin cementum has not been studied previously. Synchrotron X-ray fluorescence (SXRF) enables rapid scanning of large tissue areas with high resolution of elemental distributions. First, we used SXRF to map calcium, phosphorus, strontium and zinc distributions in great ape dentine and cementum. At higher resolution, we compared zinc and strontium distributions in cellular and acellular cementum in regions where clear incremental markings were expressed. We then mapped trace element distributions in fossil hominin dentine and cementum from the 1.55–1.65 million year old site of Koobi Fora, Kenya. Zinc, in particular, is a precise marker of cementum increments in great apes, and is retained in fossil hominin cementum, but does not correspond well with the more diffuse fluctuations observed in strontium distribution. Cementum is unusual among mineralized tissues in retaining so much zinc. This is known to reduce the acid solubility of hydroxyapatite and so may confer resistance to resorption by osteoclasts in the dynamic remodelling environment of the periodontal ligament and alveolar bone.

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Zinc mapping in bone tissues by histochemistry and synchrotron radiation–induced x-ray emission: correlation with the distribution of alkaline phosphatase

Cited by 75 publications

References 39 publications

Pb distribution in bones from the Franklin expedition: synchrotron X-ray fluorescence and laser ablation/mass spectroscopy

Pb distribution in bones from the Franklin expedition: synchrotron X-ray fluorescence and laser ablation/mass spectroscopy

Accumulation of persistent tungsten in bone as in situ generated polytungstate

Incremental distribution of strontium and zinc in great ape and fossil hominin cementum using synchrotron X-ray fluorescence mapping

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