Zinc-induced DNA damage and the distribution of metals in the brain of grasshoppers by the comet assay and micro-PIXE

Augustyniak, Maria; Juchimiuk, Jolanta; Przybyłowicz, W.J.; Mesjasz‐Przybyłowicz, Jolanta; Babczyńska, Agnieszka; Migula, P.

doi:10.1016/j.cbpc.2006.09.003

Cited by 38 publications

(26 citation statements)

References 37 publications

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“…This means that from the moment that eggs are laid until they hatched, the embryos were subjected to similar conditions. Such a result can be evidence of permanent changes that are possibly consolidated by natural selection [34]. The results described above are in accordance with the results obtained in the present studies for a Cd-exposed strain.…”

Section: Discussionsupporting

confidence: 92%

Phenotypic Plasticity, Epigenetic or Genetic Modifications in Relation to the Duration of Cd-Exposure within a Microevolution Time Range in the Beet Armyworm

et al. 2016

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In the case of the pests inhabiting metal polluted or fields where the use of pesticides is common, a natural selection of resistant individuals can occur. This may pose serious problems for humans, agriculture, as well as the economies of many countries. In this study, the hypothesis that multigenerational (120 generations) exposure to cadmium of a beet armyworm population could be a selecting factor toward a more efficient DNA protection was verified. The hemocytes of individuals from two culture strains (control and Cd-exposed) were treated with H2O2 (a DNA-damaging agent) or PBS (reference). The level of DNA damage was assessed using the Comet assay immediately and 5, 15 and 30 min. after the treatment. The immediate result of the contact with H2O2 was that the level of DNA damage in the hemocytes of the insects from both strains increased significantly. However, in the cells of the Cd-exposed individuals, the level of DNA damage decreased over time, while in the cells from the control insects it remained at the same level with no evidence of repair. These results suggest that efficient defense mechanisms may exist in the cells of insects that have prolonged contact with cadmium. Some evolutionary and trade-off aspects of the phenomenon are discussed. In a wider context, comparing the results obtained in the laboratory with field studies may be beneficial for understanding basic mechanisms of the resistance of an organism. To summarize, the high potential for the repair of DNA damage that was observed in the insects from the cadmium strain may confirm the hypothesis that multigenerational exposure to that metal may possibly contribute to the selection of insects that have a wider tolerance to oxidative stress. However, our investigations of polymorphism using AFLP did not reveal differences between the two main insect strains.

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Section: Discussionsupporting

confidence: 92%

Phenotypic Plasticity, Epigenetic or Genetic Modifications in Relation to the Duration of Cd-Exposure within a Microevolution Time Range in the Beet Armyworm

et al. 2016

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“…According to Jackson et al (2006), the complexity of sample preparation and instrumental techniques, the expense of instrument, and the number of analyte ions that can be determined are the important criteria for selecting a surface analytical technique. Based on literatures reported previously, besides the abovementioned fluorescence microscopic techniques, proton-induced X-ray emission (PIXE) (Thong et al, 1999;Robertson et al, 2002;Augustyniak et al, 2006;Reinert et al, 2007), microprobe synchrotron X-ray fluorescence (ASXRF) (Ide-Ektessabi, Kawakami, & Watt, 2004;Flinn et al, 2005;Serpa et al, 2008), synchrotron X-ray absorption spectroscopy (SXAS) (Collingwood et al, 2005;Mikhaylova et al, 2005), secondary ion mass spectrometry (SIMS) (Burns, 1981;Clerc, Fourre, & Fragu, 1997;Aranyosiova et al, 2008), and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) (Feldmann, Kindness, & Ek, 2002;Becker et al, 2005aBecker et al, ,b, 2007aBecker et al, ,b, 2008aJackson et al, 2006;Dobrowolska et al, 2008;Sela et al, 2007;Zoriy et al, 2008) are the most widely applied surface analytical techniques for elemental imaging in brain tissues.…”

Section: Surface Analytical Techniquesmentioning

confidence: 99%

In vivomonitoring of the transfer kinetics of trace elements in animal brains with hyphenated inductively coupled plasma mass spectrometry techniques

Su¹,

Sun²,

Tzeng³

et al. 2009

Mass Spectrom. Rev.

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The roles of metal ions to sustain normal function and to cause dysfunction of neurological systems have been confirmed by various studies. However, because of the lack of adequate analytical method to monitor the transfer kinetics of metal ions in the brain of a living animal, research on the physiopathological roles of metal ions in the CNS remains in its early stages and more analytical efforts are still needed. To explicitly model the possible links between metal ions and physiopathological alterations, it is essential to develop in vivo monitoring techniques that can bridge the gap between metalloneurochemistry and neurophysiopathology. Although inductively coupled plasma mass spectrometry (ICP-MS) is a very powerful technique for multiple trace element analyses, when dealing with chemically complex microdialysis samples, the detection capability is largely limited by instrumental sensitivity, selectivity, and contamination that arise from the experimental procedure. As a result, in recent years several high efficient and clean on-line sample pretreatment systems have been developed and combined with microdialysis and ICP-MS for the continuous and in vivo determination of the concentration-time profiles of metal ions in the extracellular space of rat brain. This article reviews the research relevant to the development of analytical techniques for the in vivo determination of dynamic variation in the concentration levels of metal ions in a living animal.

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“…It has been successfully applied to cells of various animal groups [13]. Only a few studies have been reported on DNA damage in insects, including D. melanogaster [14], and in the weevil Curculio sikkimensis [15], and in grasshoppers Chorthippus brunneus [16].…”

Section: Introductionmentioning

confidence: 99%

DNA damage in hemocytes of Schistocerca gregaria (Orthoptera: Acrididae) exposed to contaminated food with cadmium and lead

Yousef¹,

Afify²,

Hasan³

et al. 2010

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We measured in a comet assay the damage of DNA in the hemocytes of various stages of the grasshopper Schistocerca gregaria after exposing them to various doses of Cd and Pb in the food. The mechanisms of Cd and Pb toxicity for grasshopper are discussed. The accumulation of heavy metals and stage of the insect may play important roles in causing the DNA damage. S. gregaria may be considered a valuable bioindicator for evaluation the genotoxicity of environmental pollutants.

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Zinc-induced DNA damage and the distribution of metals in the brain of grasshoppers by the comet assay and micro-PIXE

Cited by 38 publications

References 37 publications

Phenotypic Plasticity, Epigenetic or Genetic Modifications in Relation to the Duration of Cd-Exposure within a Microevolution Time Range in the Beet Armyworm

Phenotypic Plasticity, Epigenetic or Genetic Modifications in Relation to the Duration of Cd-Exposure within a Microevolution Time Range in the Beet Armyworm

In vivomonitoring of the transfer kinetics of trace elements in animal brains with hyphenated inductively coupled plasma mass spectrometry techniques

DNA damage in hemocytes of Schistocerca gregaria (Orthoptera: Acrididae) exposed to contaminated food with cadmium and lead

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