Zinc finger proteins: what we know and what we would like to know

El-Baradi, T. T. A. L.; Pieler, Tomas

doi:10.1016/0925-4773(91)90015-x

Cited by 118 publications

(42 citation statements)

References 138 publications

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“…These data suggest that additional imprinted genes may be found near Peg3 in the proximal region of Mmu7, and the ZNF genes that surround Peg3 in Mmu7 represent excellent candidates as additional members of this newly mapped imprinted region. Because Peg3 and other ZNFs are putative transcription factors (El-Baradi and Pieler 1991), it will be interesting to see whether many downstream genes controlled by these transcription factors are also affected indirectly by the imprinting.…”

Section: Discussionmentioning

confidence: 99%

“…The Peg3 transcription unit encodes a Krü ppel-type (C 2 H 2 ) zinc finger-containing (ZNF) protein, most of which are thought to function as transcription factors (El-Baradi and Pieler 1991;Pieler and Bellefroid 1994). Genetic studies have indicated that there are three different imprinted regions along the length of mouse chromosome 7 (Mmu7), located in proximal, central, and distal regions of the chromosome, respectively (Searle and Beechey 1990;Cattanach et al 1992).…”

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confidence: 99%

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The Human Homolog of a Mouse-Imprinted Gene, Peg3, Maps to a Zinc Finger Gene-Rich Region of Human Chromosome 19q13.4

et al. 1997

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Peg3 (paternally expressedgene 3) is the first imprinted gene detected in the proximal region of mouse chromosome 7. Because imprinting is a trait that is generally conserved among mammals, and imprinted domains generally encompass several adjacent genes, expression patterns and chromosomal environment of the human counterpart of Peg3 are of special interest. In this study we have localized human PEG3 ∼2 Mb proximal of the telomere of chromosome 19q, within a region known to carry large numbers of tandemly clustered Krüppel-type zinc finger-containing (ZNF) genes. Peg3 also encodes a Krüppel-type ZNF protein but one that is distinguished from other ZNF gene products by the fact that it carries two novel proline-rich motifs. Comparison between mousePeg3 and partial human PEG3 gene sequences revealed a high level of conservation between the two species, despite the fact that one of the two proline-rich repeats is absent from the human gene. Our data demonstrate that the human gene is expressed at highest levels in ovary and placenta; mouse Peg3, by contrast, is transcribed at highest levels in the adult brain. These comparative mapping, sequencing, and expression data provide the first clues to the potential activities of PEG3, and generate new tools to aid in the analysis of structure and function of a potentially new imprinted domain located in human chromosome 19q13.4 and mouse chromosome 7.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

The Human Homolog of a Mouse-Imprinted Gene, Peg3, Maps to a Zinc Finger Gene-Rich Region of Human Chromosome 19q13.4

et al. 1997

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“…A zinc finger motif was first identified in the Xenopus aevis RNA polymerase III transcription factor TFIIIA [3,4]. qince then, several zinc finger genes involved in gene regulaion have been identified in a variety of animals and plants 5,6]. ~Corresponding author.…”

Section: T Introductionmentioning

confidence: 99%

Zfp60, a mouse zinc finger gene expressed transiently during in vitro muscle differentiation

et al. 1996

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t. IntroductionThe formation of mature muscle fibers requires the coordin-,tted expression of many different genes coding for the myo~ube-specific proteins. These functions are governed by a set .)f regulatory genes [1]. Myogenic cell lines and myoblast 0rimary cultures can mimic many of the steps characteristic )f myogenic differentiation. The possibility to follow and to • ~tudy the in vitro transition of myoblast cells to myotube !~rovides a useful system to study gene regulation during myo-!genesis. In the last few years, several muscle regulatory genes !lave been identified and characterized such as many members • )f the MyoD gene family. This family of regulatory genes ~MyoD, myogenin, Myf5 and MRF4) share the same evoluionarily conserved domains, a basic region involved in DNA ~inding to the specific target sequence (E box), and a helix oop helix (HLH) implicated in homodimerization and/or leterodimerization with other regulatory protein [2]. The badc idea of this work is the isolation and characterization of )ther regulatory genes, involved in myogenesis, coding for ~roteins with the 'zinc finger' motif as a DNA binding donain. A zinc finger motif was first identified in the Xenopus aevis RNA polymerase III transcription factor TFIIIA [3,4]. qince then, several zinc finger genes involved in gene regulaion have been identified in a variety of animals and plants 5,6]. ~Corresponding author. Fax: (39) (6) 446 2891. E-mail: claudio@dbu.uniroma 1 .itIn a previous work we reported on the characterization of three zinc finger genes, isolated from an adult mouse skeletal muscle cDNA library [7]. In this work we describe one of those genes, termed Mfg3 and presently renamed Zfp60 according to the international convention. We determine the complete cDNA coding sequence and the predicted amino acid sequence of the ZFP60 protein. This protein is characterized by a cluster of 19 zinc finger motifs at the C-terminus and by the two Kruppel associated boxes (KRAB) A and B at the N-terminus. These modules have been shown to define a subfamily of multifinger proteins [8,9] and to be associated with transcriptional repression activity [10 13]. The expression patterns of Zfp60, MyoD and myosin heavy chain (MHC) mRNAs have been followed and compared during myoblast C2 cell line differentiation. We also show that the bacterial expressed Zfp60 protein is able to bind DNA only in presence of zinc ions. Using restriction length polymorphism (RFLP) we assigned the Zfp60 locus to the murine chromosome 7, at about 10 cM from the centromere, where a cluster of Zfp loci maps. This region shows homology with human chromosome 19q13.

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“…The zinc finger is one of the structural motifs characteristic of DNA binding proteins [9]. It consists of a characteristic arrangement of cysteine and histidine residues which are thought to co-ordinate Zn 2+ ions.…”

Section: Introductionmentioning

confidence: 99%

“…Some of these have been identified as transcription factors such as TFIIIA [8] and Spl [14], developmental genes in Drosophila such as Kruppel [15] and genes implicated as oncogenes [16,17] or tumour suppressor genes [18]. Many other ZFP genes have been isolated by low stringency hybridization or hybridization with consensus oligonucleotide probes [9,12,13,19]. Although the presence of zinc fingers is often taken to be indicative of a role in transcription, some ZFPs may have a structural role, while others may bind exclu-*Corresponding author.…”

Section: Introductionmentioning

confidence: 99%

Zfy1 encodes a nuclear sequence‐specific DNA binding protein

1995

FEBS Letters

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Zfyl is a mouse Y chromosomal gene encoding a zinc finger protein which is thought to have some function during spermatogenesis. Here we show that, when introduced into tissue culture cells, Zfyl is targeted to the nucleus. Two independent signals are present within the protein for nuclear localization. This nuclear Zfyl protein is able to bind strongly to DNA-ceHulose and, using site-selection assays, we have identified specific Zfyl DNA binding sites. Taken together these results suggest that Zfyl is a nuclear-located sequence-specific DNA binding protein which functions during spermatogenesis.

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Zinc finger proteins: what we know and what we would like to know

Cited by 118 publications

References 138 publications

The Human Homolog of a Mouse-Imprinted Gene, Peg3, Maps to a Zinc Finger Gene-Rich Region of Human Chromosome 19q13.4

The Human Homolog of a Mouse-Imprinted Gene, Peg3, Maps to a Zinc Finger Gene-Rich Region of Human Chromosome 19q13.4

Zfp60, a mouse zinc finger gene expressed transiently during in vitro muscle differentiation

Zfy1 encodes a nuclear sequence‐specific DNA binding protein

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