2018
DOI: 10.1111/pbi.12941
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Abstract: SummarySequence‐specific nucleases have been used to engineer targeted genome modifications in various plants. While targeted gene knockouts resulting in loss of function have been reported with relatively high rates of success, targeted gene editing using an exogenously supplied DNA repair template and site‐specific transgene integration has been more challenging. Here, we report the first application of zinc finger nuclease (ZFN)‐mediated, nonhomologous end‐joining (NHEJ)‐directed editing of a native gene in… Show more

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Cited by 58 publications
(26 citation statements)
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“…In the future, understanding the relationship between multiple genes affecting yield components will most likely require multiplex genome editing of the different targets across several genetic backgrounds (Shen et al ). This will involve not only knock‐out mutations but also allele replacement and promoter manipulations (Puchta ; Rodríguez‐Leal et al ; Li et al ; Ran et al ).…”
Section: Interactionsmentioning
confidence: 99%
“…In the future, understanding the relationship between multiple genes affecting yield components will most likely require multiplex genome editing of the different targets across several genetic backgrounds (Shen et al ). This will involve not only knock‐out mutations but also allele replacement and promoter manipulations (Puchta ; Rodríguez‐Leal et al ; Li et al ; Ran et al ).…”
Section: Interactionsmentioning
confidence: 99%
“…Both circular and linear DNA can be introduced into plant cells by particle bombardment, and the co-delivery of different DNA fragments or plasmids is quite efficient because they can be coated onto the same microparticles [121,122]. A recent study in wheat demonstrated the ZFN-mediated precise editing of the three homoeologous ALS genes following bombardment with the ZFN construct and a short dsDNA donor with compatible overhangs [76].…”
Section: Delivery Methodsmentioning
confidence: 99%
“…TALENs and the CRISPR/Cas9 system have been used for nucleotide exchange in the rice gene encoding acetolactate synthase ( ALS ), generating plants resistant to bispyribac-sodium [75]. Furthermore, ZFNs have been used for nucleotide exchange in the wheat ALS gene to confer resistance to imidazolinone herbicides [76]. By delivering the CRISPR/Cas9 components either as DNA or RNP in combination with single-strand oligonucleotides, the maize ALS2 gene was also edited and chlorsulfuron-resistant plants were obtained [77,78].…”
Section: Outcomes Of Genome Editingmentioning
confidence: 99%
“…In this case, a strain of the Bean Yellow Dwarf Virus was modified so that only the elements essential for the method remained and the artificial repair template was amplified with high frequency [110]. In barley and wheat, precise editing has been achieved either only at the cellular level [98] or has the limiting prerequisite that the obtained genetic modification leads to an in vitro selectable trait, e.g., an herbicide resistance [111].…”
Section: Methodological Aspects Of Cas Endonuclease Technologymentioning
confidence: 99%