Zinc finger gene nolz1 regulates the formation of retinal progenitor cells and suppresses the Lim3/Lhx3 phenotype of retinal bipolar cells in chicken retina

Abstract

We conclude that Nolz1 has multiple functions during development and suggest a mechanism in which Nolz1 initially regulates the proliferation state of the retinal progenitor cells and then acts as a repressor that suppresses the Lim3/Lhx3 bipolar cell phenotype at the time of bipolar cell differentiation. Developmental Dynamics 247:630-641, 2018. © 2017 Wiley Periodicals, Inc.

Transcriptome analysis of the zebrafishatoh7−/−Mutant,lakritz, highlights Atoh7‐dependent genetic networks with potential implications for human eye diseases

“…Genetic evidences for the GTPase and zinc finger transcriptional repressor encoding genes, gnl2 and znf503 (NOLZ1) as eye disorders-related genes are still missing but studies support their functional requirement for retinal developmental processes, including proper cell cycle exit of RPCs during RGC differentiation. 72,73 Notably, tubgcp4, gnl2 and znf503 were implicated in the regulation of cytokinesis late in mitosis. [71][72][73] This observation further indicates Atoh7 requirements for the regulation of cell cycle progression, at least in RPCs.…”

“…Single pairs of eyes were dissected from single embryos at 25,28,35,48,72, and 96 hpf and snap-frozen in liquid nitrogen, and stored at −80°C. Embryos older than 28 hpf were first anesthetized for 5-10 minutes in ethyl 3-aminobenzoate methanesulfonate (MS-222) (Sigma-Aldrich) in E3 medium.…”

“…This analysis consistently underscored "neural retina development" (GO:0 003 407) as the enriched term both by Metascape (not shown) and Kobas (Table S3). This category contains nine statistically significantly differentially expressed genes (including atoh7), which comprehend early expressed eye-field transcription factors (EFTFs, rx1, and six6a), [62][63][64][65] stress response and extracellular matrix remodeling factors (hsp70.1, mmp14a), [66][67][68][69] chromatin regulators (smarca5) 70 as well as microtubules organizers and cell cycle regulatory proteins (tubgcp4, znf503, and gnl2) [71][72][73] (Table 2). Other (albeit less significant) relevant biological processes emerging from this analysis were "cell cycle process" (GO:0022402), "chromatin remodeling" (GO:0006338), and "Wnt signaling pathway" (GO:0016055) (Figure 3 and Table S2).…”

Transcriptome analysis of the zebrafishatoh7−/−mutant,lakritz, highlights Atoh7-dependent genetic networks with potential implications for human eye diseases

“…Table 3). This category contains 9 statistically significantly differentially expressed genes (including atoh7), which comprehend early expressed eye-field transcription factors (EFTFs, rx1 and six6a) [94][95][96][97] , stress response and extracellular matrix remodelling factors (hsp70.1, mmp14a) [98][99][100][101] , chromatin regulators (smarca5) 102 as well as microtubules organisers and cell cycle regulatory proteins (tubgcp4, znf503, gnl2) [103][104][105] . Other (albeit less significant) relevant biological processes emerging from this analysis were "cell cycle process" (GO:0022402), "chromatin remodeling" (GO:0006338) and "Wnt signaling pathway"…”

Generation and characterization of Lhx3GFP reporter knockin and Lhx3loxP conditional knockout mice

“…Published studies have reported that Lhx3 is expressed in hindbrain, pineal gland, spinal cord, and pituitary (Seidah et al, ) as well as in retinal bipolar cells (Balasubramanian, Bui, Ding, & Gan, ; Blixt, Konjusha, Ring, & Hallbook, ; Elshatory et al, ) and the inner ear (Huang et al, ; Hume, Bratt, & Oesterle, ; Rajab et al, ). Deletion of the two LIM domains and a part of the HD of LHX3 by conventional knockout approach in mice leads to abnormal Rathke's pouch and embryonic death at birth or neonatal death within 24 h after birth and abolishes the expression of Pit‐1 , TSH , GSU , and GH (Sheng et al, ).…”