Zinc–desferrioxamine reduces damage to lenses exposed to hyperbaric oxygen and has an ameliorative effect on catalase and Na, K-ATPase activities

Schaal, Shlomit; Beiran, Itzchak; Bormusov, E.; Chevion, Mordechai; Dovrat, A.

doi:10.1016/j.exer.2006.10.019

Cited by 6 publications

(7 citation statements)

References 29 publications

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“…While DFO proved only partially beneficial in salvaging the lens periphery from oxidative damage, Zn/DFO led to complete protective benefit to the lens anterior pole, the part most relevant for sight preservation [32,33]. These observations were in accord with other publications demonstrating the beneficial effects of iron chelating agents against oxidative insult-induced tissue damage [7,8,[34][35][36][37].…”

Section: Resultssupporting

confidence: 81%

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Antioxidants Prevention of Diabetic Damage in the Organ Culture Bovine Lenses

Dovrat¹

2012

Metabolomics

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Oxidative stress represents a mechanism which could lead to diabetic cataract. We exposed bovine lenses in culture conditions for two weeks to high glucose concentration (450 mg%) and investigated the damage to the lens and possible protection by special antioxidants -N-acetyl-L-cysteine (NAC) and the zinc complex of desferrioxamine (DFO), a selective chelator for iron. We monitored the optical quality of the lenses and the oxidation of the epithelium with dichlorofluorescein (DCF) assay, as well as the changes in lens proteins profile by 2D gel electrophoresis. Under high glucose changes in lens focal length, increased oxidation, and changes in lens crystalline were observed. NAC and Zn-DFO nearly completely protected the lenses; DFO showed only partial protection. The results demonstrated that antioxidants should be considered as treatment modality protecting the lens from high glucose damage. It is proposed that a combination of NAC and Zn/DFO could prove highly efficient.the presence of high glucose concentrations (450 mg %), mimicking the diabetic state, and examined the protection bestowed by the DFO complexes and NAC, on the injurious processes on the lenses. We used a unique system of intact bovine lenses, maintained for a longterm, under culture conditions. This system allows for direct exposure to a pre-set glucose concentration, and monitoring the effects on lens transparency with a highly sensitive optical method [20]. The system can detect early optical damage to lenses, which cannot be detected by other methods often used. At the completion of the culture period lens epithelium and lens proteins were analyzed.It was anticipated that the extent of protection exerted on the lens, in this system, will be indicative of the involvement of iron as a catalyst and of free radicals as causative agents, in the damage to the diabetic lens. Materials and Methods Lens organ culture systemLenses were excised in a delicate operation from eyes obtained from 1-year-old male calves under sterile conditions, 2-4 hours after enucleating. From each animal one eye is used for experimental treatment and the other eye serves as control. Each lens is placed in a specially designed culture container, which we have developed [21]. The culture medium consists of M199 with Earl's balanced salt solution, supplemented with 5.96g/L HEPES, 3% dialyzed fetal calf serum and antibiotics (penicillin 100 U/ml and streptomycin 0.1 mg/ ml). Our intact lens culture system mimics the lens conditions inside the eye and makes it possible to keep lenses for long-term studies for

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Section: Resultssupporting

confidence: 81%

“…The disadvantage of DFO in these studies stems from its limited tissue penetration [38,39]. Indeed, Zn/DFO easily infiltrates into cells and tissues [9,32,40] and is, thus, a better protective drug, as is evident also under conditions that simulate diabetes.…”

Section: Resultsmentioning

confidence: 99%

Antioxidants Prevention of Diabetic Damage in the Organ Culture Bovine Lenses

Dovrat¹

2012

Metabolomics

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“…This may be due to the higher CAT levels in the cortex+epithelium and the fact that the epithelium is the first site of oxygen reaction in the lens. 9,40 …”

Section: Discussionmentioning

confidence: 99%

Oxidative Responses Induced by Pharmacologic Vitreolysis and/or Long-term Hyperoxia Treatment in Rat Lenses

Li¹,

Zhang²,

Ding³

et al. 2013

Current Eye Research

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Purpose The aim of the study was to investigate the protective effects of intact vitreous gel on the lens after pharmacologic vitreolysis and hyperoxia exposure in rats in vivo. Methods Eyes of Sprague-Dawley rats were induced to posterior vitreous detachment (PVD) by pharmacologic vitreolysis, and the rats with and without PVD were treated with hyperoxia 3 h per day for 5 months. Lens transparency was monitored by a slit-lamp biomicroscope. A series of biochemical measurements were made in extracts of the lens cortex and nucleus. Ascorbate levels were measured in the aqueous and vitreous humors. Results No significant differences in lens transparency or morphology were observed in all groups, and no significant biochemical changes were observed in the cortex or nucleus of lenses of the PVD group. In the lens nucleus, the values of water-soluble protein concentration in PVD + hyperoxia group were lower than that of the PVD group. The levels of water-soluble proteins, glutathione (GSH) and ascorbate decreased in the hyperoxia group with an intact vitreous body. Vitreolysis enhanced the effect of hyperoxia, decreasing soluble protein, GSH and ascorbate below the levels seen in eyes with vitreolysis alone. The levels of antioxidants and soluble proteins were lower in the lens nucleus, and the effects of vitreolysis plus hyperoxia were more significant in the nucleus. Hyperoxia and hyperoxia plus vitreolysis reduced catalase activity and increased oxidized GSH to a greater extent in the lens cortex, although these treatments increased protein-GSH mixed disulfides in both regions. Long-term hyperoxia also lowered ascorbate levels in the vitreous and aqueous humors, an effect that was enhanced by vitreolysis. Conclusions Exposure to excess molecular oxygen produces significant oxidative damage to the lens, especially the lens nucleus. These effects were enhanced by pharmacologic vitreolysis, indicating that intact vitreous gel protects the lens from oxidative damage.

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“…Êàê óñòàíîâëåíî ê íàñòîÿùåìó âðåìåíè, îêèñëèòåëüíûé ñòðåññ èãðàåò ðîëü â ïàòîãåíåçå ìíîãèõ çàáîëåâàíèé, íàïðèìåð, äåãåíåðàòèâíûõ (âêëþ÷àÿ àòåðîñêëåðîç, áîêîâîé àìèîòðîôè÷åñêèé ñêëåðîç), ãåìîëèòè÷åñêîé àíåìèè, êàðäèîìèîïàòèè, àðòðèòîâ, äà è â ñàìîì ñòàðåíèè [8,25]. Îôòàëüìîëîãèÿ çäåñü íå èñêëþ÷åíèå -ñåíèëüíàÿ êàòàðàêòà, âîçðàñòíàÿ ìàêóëîäèñòðîôèÿ, ãåðïåòè÷åñêèé êåðàòèò, äèàáåòè÷åñêàÿ ðåòèíîïàòèÿ èëëþñòðèðóþò ýòî óòâåðaeäåíèå [19,31,49,50,51,61]. Íî îñîáî îòìå-òèì, ÷òî äëÿ ñåò÷àòêè, ÿâëÿþùåéñÿ ìåòàáîëè÷åñêè íàèáîëåå àêòèâíîé ñòðóêòóðîé â îðãàíèçìå ÷åëîâåêà [23], ýòîò ñòðåññ îñîáåííî îïàñåí.…”

Section: òàáëèöàunclassified

“…Ó÷èòûâàÿ âñå âûøåñêàçàííîå, íåóäèâèòåëüíî, ÷òî ó aeèâîòíûõ â íàøèõ îïûòàõ äàaeå íåêîòîðîå ñíèaeåíèå àêòèâíîñòè íåñêîëüêèõ ôåðìåíòîâ, îáåçâðåaeèâàþùèõ àêòèâíûå ôîðìû êèñëîðîäà, óaeå îêàçûâàåòñÿ âåñüìà îïàñíûì. Äîñòàòî÷íî íàïîìíèòü îá îáðàçîâàíèè âûñîêîòîêñè÷íîãî ãèäðîêñèëüíîãî ðàäèêàëà èç ïåðåêèñè âîäîðîäà â õîäå ðåàêöèè Ôåíòîíà [49,50], èíòåíñèâíîñòü ïðîòåêàíèÿ êîòîðîé óâåëè÷èâàåòñÿ õîòÿ áû áëàãîäàðÿ ñíèaeåíèþ àêòèâíîñòè ÃÏÎ è, â èçâåñòíîé ìåðå, êàòàëàçû, ÷òî è áûëî ïðîäåìîíñòðèðîâàíî íàìè âûøå.…”

Section: òàáëèöàunclassified

The state of the reticulated and vascular eye membranes of rabbit with diabetes mellitus, modeled by Dithi-zone. Information 2. Antioxidant enzymes and su¬peroxide oxygen

Maltsev¹,

Zborovskaya²,

Dorokhova³

2012

Oftalmol Zh

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ÃÓ «Èíñòèòóò ãëàçíûõ áîëåçíåé è òêàíåâîé òåðàïèè ÍÀÌÍ Óêðàèíû» Ïðè ³íòîêñèêàö³¿ êðîëèê³â äèòèçîíîì âaeå ÷åðåç 6=7 ãîäèí ï³ñëÿ éîãî ââåäåííÿ (ñòàä³ÿ ã³ïîãë³êå-ì³¿) ìàº ì³ñöå çíèaeåííÿ àêòèâíîñò³ êàòàëàçè (íà 12,6 %) ³ ãëþòàò³îíïåðîêñèäàçè (íà 18,0 %). ×åðåç 33-36 ãîäèí (ïî÷àòêîâèé ïåð³îä ñòàä³¿ âòîðèííî¿ ñò³éêî¿ ã³ïåðãë³êåì³¿) âèÿâëÿºòüñÿ òàêîae çíèaeåííÿ àêòèâíîñò³ ñóïåðîêñèääèñìóòàçè (íà 16,7 %). Öå ñïðèÿº ðîçâèòêó îêèñëþâàëüíîãî ñòðåñó â ñ³ò÷àñò³é òà ñóäèíí³é îáîëîíêàõ îêà òâàðèí ³ º, ïðèíàéì³, îäíîþ ç ïðè÷èí âèíèêàþ÷î¿ ïîò³ì ä³àáåòè÷íî¿ ðåòè-íîïàò³¿ ç ÿñêðàâî âèðàaeåíèìè ÿâèùàìè íåéðîäåãåíåðàö³¿.

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Zinc–desferrioxamine reduces damage to lenses exposed to hyperbaric oxygen and has an ameliorative effect on catalase and Na, K-ATPase activities

Cited by 6 publications

References 29 publications

Antioxidants Prevention of Diabetic Damage in the Organ Culture Bovine Lenses

Antioxidants Prevention of Diabetic Damage in the Organ Culture Bovine Lenses

Oxidative Responses Induced by Pharmacologic Vitreolysis and/or Long-term Hyperoxia Treatment in Rat Lenses

The state of the reticulated and vascular eye membranes of rabbit with diabetes mellitus, modeled by Dithi-zone. Information 2. Antioxidant enzymes and su¬peroxide oxygen

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