Zinc Content in Epididymal Spermatozoa of Metoclopramide-Treated Rats

Kamińska, Barbara; Roźéwicka, L; Dominiak, Bernard C.; Mielnicka, M.; Mikulska, Danuta

doi:10.1111/j.1439-0272.1987.tb01928.x

Cited by 16 publications

(2 citation statements)

References 15 publications

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“…During the epididymal transit of rat spermatozoa, approximately 60% of Zn 2+ content is lost from epididymal spermatozoa [112,113] and reabsorbed by the epididymal epithelium [114]. This maturation step allows for the oxidation of sulfhydryl groups thus stiffening the ODF, and is mandatory to obtain functional ODF, a prerequisite for progressive motility.…”

Section: Role Of Zinc In Sperm Maturationmentioning

confidence: 99%

Spermatozoan Metabolism as a Non-Traditional Model for the Study of Huntington’s Disease

Lawlor

Zigo

Kerns

et al. 2022

IJMS

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Huntington’s Disease (HD) is a fatal autosomal dominant neurodegenerative disease manifested through motor dysfunction and cognitive deficits. Decreased fertility is also observed in HD animal models and HD male patients, due to altered spermatogenesis and sperm function, thus resulting in reduced fertilization potential. Although some pharmaceuticals are currently utilized to mitigate HD symptoms, an effective treatment that remedies the pathogenesis of the disease is yet to be approved by the FDA. Identification of genes and relevant diagnostic biomarkers and therapeutic target pathways including glycolysis and mitochondrial complex-I-dependent respiration may be advantageous for early diagnosis, management, and treatment of the disease. This review addresses the HD pathway in neuronal and sperm metabolism, including relevant gene and protein expression in both neurons and spermatozoa, indicated in the pathogenesis of HD. Furthermore, zinc-containing and zinc-interacting proteins regulate and/or are regulated by zinc ion homeostasis in both neurons and spermatozoa. Therefore, this review also aims to explore the comparative role of zinc in both neuronal and sperm function. Ongoing studies aim to characterize the products of genes implicated in HD pathogenesis that are expressed in both neurons and spermatozoa to facilitate studies of future treatment avenues in HD and HD-related male infertility. The emerging link between zinc homeostasis and the HD pathway could lead to new treatments and diagnostic methods linking genetic sperm defects with somatic comorbidities.

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Section: Role Of Zinc In Sperm Maturationmentioning

confidence: 99%

Spermatozoan Metabolism as a Non-Traditional Model for the Study of Huntington’s Disease

Lawlor

Zigo

Kerns

et al. 2022

IJMS

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“…The capacity for progressive motility is gained solely during maturation of the spermatozoa as they are transported through the epididymis (Haidl et al, 1994). In the course of that journey, the zinc content of the sperm is reduced by approximately 60% (Kaminska et al, 1987), which leads to the increased stabilization of the outer dense fiber (ODF) proteins that is induced when sulfhydryl groups are oxidized to form disulfide bridges (Calvin et al, 1973). In our study, prolonging the time from ejaculation to analysis to more than 1 hour was associated with significantly lower levels of zinc in the semen samples.…”

Section: Discussionmentioning

confidence: 99%

Effects of Ejaculation‐to‐Analysis Delay on Levels of Markers of Epididymal and Accessory Sex Gland Functions and Sperm Motility

Elzanaty¹,

Malm²

2007

Journal of Andrology

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This study aimed to examine the association between the interval from ejaculation to analysis and epididymal and accessory sex gland function in relation to sperm motility. Ejaculates from 1079 men assessed for infertility were analyzed according to World Health Organization guidelines. Biochemical markers were measured in semen to assess the function of the epididymis (neutral a-glucosidase [NAG]), prostate (prostate-specific antigen [PSA] and zinc), and seminal vesicles (fructose). Three groups were defined according to time from ejaculation to analysis: G #30 (24-30 minutes), G 31-60 (31-60 minutes), and G .60 (63-180 minutes). The proportion of progressively motile sperm was significantly lower in G .60 than in G #30 (mean difference, 8.0%; 95% confidence interval [CI], 2.0%-13%) or G 31-60 (mean difference, 6.0%; 95% CI, 1.0%-12%). The proportion of rapid progressive sperm motility was significantly higher in G #30 compared with G (mean difference, 3.0%; 95% CI, 1.0%-5.0%) and G .60 (mean difference, 6.0%; 95% CI, 1.0%-10%). Sperm morphology and viability did not vary significantly between the groups. However, PSA levels in G .60 were 29% and 31% significantly lower than in G #30 (95% CI, 3.0%-54%) and G 31-60 (95% CI, 7.0%-58%), respectively. Moreover, men in G .60 had 29% and 17% significantly lower zinc compared with those in G #30 (95% CI, 4.0%-69%) and G 31-60 (95% CI, 4.0%-64%), respectively. Levels of NAG and fructose did not differ significantly between the groups. There were negative associations between the ejaculation-to-analysis interval and sperm motility and levels of PSA and zinc. In male infertility assessments, semen analysis should be performed within 60 minutes of ejaculation.Key words: Biochemical markers, morphology, semen analysis, viability.J Androl 2007;28:847-852 S perm motility is believed to be one of the most important parameters for evaluating the fertilizing ability of ejaculated spermatozoa both in vivo and in vitro (Bongso et al, 1989;Eimers et al, 1994;Donnelly et al, 1998;Larsen et al, 2000;Hirano et al, 2001). The ability of the spermatozoa to move is determined by a multitude of factors, including contributions from properly functioning epididymal and accessory sex glands (Malm et al, 2000;Elzanaty et al, 2002). The qualities of semen are also influenced by several factors, such as the time elapsed from ejaculation/collection to analysis. However, only 1 study has addressed this issue (Mortimer et al, 1982), and the results suggested that sperm motility and viability decline when the interval from ejaculation to analysis exceeds 2 hours.The analysis of semen quality plays an important role in clinical decisions regarding the strategy for infertility treatment. Therefore, it is essential to minimize the impact of variation in sample delivery and analytic conditions on the outcome of this testing. In the World Health Organization (WHO) manual (1999), which is the most accepted guideline for semen analysis, it is recommended that assessment of semen in infertilit...

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Histochemical localization of zinc ions in the epididymis of the rat

et al. 1996

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In the present study, the autometallograpic zinc sulphide technique, an improved version of the original Timm sulphide-silver method, was used. This technique reveals a particular pool of ionic zinc that is chelatable by diethyldithiocarbamate. At the light microscopical level, no reaction for zinc was found in tissues of young prepubertal rats. In adult mating and non-mating rats low zinc staining was found in the head and intermediate epididymis whereas the tail of the epididymis demonstrated high levels of zinc ions. Sections from the epididymal tail revealed a 'compartmentalization', based on pronounced differences in staining intensity along the epididymal ducts. At higher magnification zinc ions were found in the apical part of the principal cell and in the lumen. At the ultrastructural level autometallographic grains were located in vesicles and in lysosome-like structures of the apical parts of the principal cells. The luminal grains were found either associated with sperm cells, with the surface of the large microvilli (stereocilia), or free in the seminal fluid. The variation in content of zinc ions in the epididymal epithelium and lumen suggests that zinc ions are secreted into the lumen from the epididymal tail and may somehow be involved in maturation of the sperm cells.

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Zinc Content in Epididymal Spermatozoa of Metoclopramide-Treated Rats

Cited by 16 publications

References 15 publications

Spermatozoan Metabolism as a Non-Traditional Model for the Study of Huntington’s Disease

Spermatozoan Metabolism as a Non-Traditional Model for the Study of Huntington’s Disease

Effects of Ejaculation‐to‐Analysis Delay on Levels of Markers of Epididymal and Accessory Sex Gland Functions and Sperm Motility

Histochemical localization of zinc ions in the epididymis of the rat

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