Zinc and Copper Reduce Conjugative Transfer of Resistance Plasmids from Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i>

Buberg, May Linn; Witsø, Ingun Lund; L’Abée-Lund, Trine M.; Wasteson, Yngvild

doi:10.1089/mdr.2019.0388

Cited by 26 publications

(27 citation statements)

References 58 publications

(64 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The rise in multi-drug resistant bacteria has sparked concern around the world, especially in Gram-negative pathogens (Young, 2013). Our previous work showed that zinc could block the emergence of new antibiotic resistance in vitro in E. coli, Klebsiella pneumoniae, and Enterobacter cloacae (Bunnell et al, 2017;Crane et al, 2018), and this was confirmed by other investigators (Buberg et al, 2020). The inhibitory effect of zinc on SOS-induced antibiotic resistance was also observed in vivo .…”

Section: Discussionsupporting

confidence: 70%

Inhibition of SOS Response by Nitric Oxide Donors in Escherichia coli Blocks Toxin Production and Hypermutation

Crane

Burke

Alvarado

2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

BackgroundPrevious reports have differed as to whether nitric oxide inhibits or stimulates the SOS response, a bacterial stress response that is often triggered by DNA damage. The SOS response is an important regulator of production of Shiga toxins (Stx) in Shiga-toxigenic E. coli (STEC). In addition, the SOS response is accompanied by hypermutation, which can lead to de novo emergence of antibiotic resistance. We studied these effects in vitro as well as in vivo.ResultsNitric oxide donors inhibited induction of the SOS response by classical inducers such as mitomycin C, ciprofloxacin, and zidovudine, as measured by assays for E. coli RecA. Nitric oxide donors also inhibited Stx toxin protein production as well as stx2 RNA in vitro and in vivo. In vivo experiments were performed with ligated ileal segments in the rabbit using a 20 h infection. The NO donor S-nitroso-acetylpenicillamine (SNAP) reduced hypermutation in vitro and in vivo, as measured by emergence of rifampin resistance. SNAP blocked the ability of the RecA protein to bind to single-stranded DNA in an electrophoretic mobility shift assay (EMSA) in vitro, an early event in the SOS response. The inhibitory effects of SNAP were additive with those of zinc acetate.ConclusionsNitric oxide donors blocked the initiation step of the SOS response. Downstream effects of this blockade included inhibition of Stx production and of hypermutation. Infection of rabbit loops with STEC resulted in a downregulation, rather than stimulation, of nitric oxide host defenses at 20 h of infection.

show abstract

Section: Discussionsupporting

confidence: 70%

Inhibition of SOS Response by Nitric Oxide Donors in Escherichia coli Blocks Toxin Production and Hypermutation

Crane

Burke

Alvarado

2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…A previous report showed that Cu 2+ and CuO nanoparticles at 100 µmol/L could decrease the horizontal transfer of plasmid-mediated ARGs due to the reduced recipient number (Zhang et al, 2019). Higher sub-MIC concentrations of Cu 2+ reduced conjugative transfer of plasmids (Buberg et al, 2020). As reported, 100 µmol/L Cu 2+ suppressed the transfer of plasmid-mediated ARG in a sludge bacterial community, probably attributed to disrupted iron-sulfur clusters of metalloenzymes and the purified fumarase A poisoning (Lin et al, 2019).…”

Section: Discussionmentioning

confidence: 63%

Copper Ions Facilitate the Conjugative Transfer of SXT/R391 Integrative and Conjugative Element Across Bacterial Genera

Song

Zuo

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

Copper can persist stably in the environment for prolonged periods. Except for inducing antibiotic resistance in bacteria, copper ions (Cu2+) can facilitate the horizontal transfer of plasmid DNA. However, whether and how Cu2+ can accelerate the conjugative transfer of SXT/R391 integrative and conjugative element (ICE) is still largely unknown. In this study, Proteus mirabilis ChSC1905, harboring an SXT/R391 ICE that carried 21 antibiotic resistance genes (ARGs), was used as a donor, and Escherichia coli EC600 was used as a recipient. Cu2+, at subinhibitory and environmentally relevant concentrations (1–10 μmol/L), significantly accelerated the conjugative transfer of SXT/R391 ICE across bacterial genera (from P. mirabilis to E. coli) (p < 0.05). The combined analyses of phenotypic tests and genome-wide sequencing indicated that reactive oxygen species (ROS) production and cell membrane permeability were critical in the enhanced conjugative transfer of SXT/R391 ICE. Furthermore, the expression of genes related to cell adhesion and ATP synthesis was also significantly upregulated on exposure to Cu2+ at a concentration of 5 μmol/L. This study clarified the potential mechanisms of Cu2+ to promote the conjugative transfer of SXT/R391 ICE, revealing the potential risk imposed by Cu2+ on the horizontal transfer of SXT/R391 ICE-mediated ARGs.

show abstract

“…Beta-lactamases, as well as integron and plasmid-encoded extended-spectrum beta-lactamases (ESBLs), are responsible for contributing resistance against cephalosporin and penicillin. Beta-lactamase inhibitors interfere with the enzyme by plasmid but not by AmpC gene (Buberg et al 2020).…”

Section: Modification and Inactivation Of Antibioticsmentioning

confidence: 99%

Mechanisms of Antimicrobial Resistance in Pseudomonas aeruginosa and a Multi-Pronged Approach to Combat its Infection in Veterinary Science and Public Health: A Review

Mustafa¹

2021

IJAB

View full text Add to dashboard Cite

Pseudomonas aeruginosa is one of the most important nosocomial pathogens associated with a variety of medical and veterinary infections and therefore, it presents a major public health threat. Different classes of antibiotics are being used to treat its infections which are increasing selective pressure to multi-drug resistance development. Resistance to antibiotics in P. aeruginosa is due to many of the common and unique mechanisms which include: reducing membrane permeability, modification or inactivation of antibiotics, alteration of enzymes, modification of target sites and over-expression of efflux systems. Over or under expression of the genes of porin channels and components of efflux systems play a major role in the resistance mechanisms of P. aeruginosa. To overcome the problem of the emergence of antibiotic resistance, many new strategies are being employed to control infections caused by P. aeruginosa. These include the use of herbs/medicinal plants and phage therapy. With the advent of modern technology, the molecular mechanisms of these alternative therapies are being elucidated and may be used in future to treat P. aeruginosa infections in humans and veterinary clinics. This review thus highlights the mechanisms of antibiotic resistance of P. aeruginosa against the commonly used antimicrobials and also some alternative strategies to control P. aeruginosa infection. © 2021 Friends Science Publishers

show abstract

Zinc and Copper Reduce Conjugative Transfer of Resistance Plasmids from Extended-Spectrum Beta-Lactamase-Producing Escherichia coli

Cited by 26 publications

References 58 publications

Inhibition of SOS Response by Nitric Oxide Donors in Escherichia coli Blocks Toxin Production and Hypermutation

Inhibition of SOS Response by Nitric Oxide Donors in Escherichia coli Blocks Toxin Production and Hypermutation

Copper Ions Facilitate the Conjugative Transfer of SXT/R391 Integrative and Conjugative Element Across Bacterial Genera

Mechanisms of Antimicrobial Resistance in Pseudomonas aeruginosa and a Multi-Pronged Approach to Combat its Infection in Veterinary Science and Public Health: A Review

Contact Info

Product

Resources

About