Zinc and copper in human semen

Stanković, Helena; Mikac-Dević, Dušanka

doi:10.1016/0009-8981(76)90013-9

Cited by 52 publications

(24 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Majority of the studies on human sperm suggest that zinc has inhibitory effect on motility [32,33]. In contrary to these reports, few studies have observed that zinc enhances sperm motility [34,35]. However, no significant change in the motility pattern was observed in the present study after supplementing zinc to the semen sample (data not shown).…”

Section: Discussioncontrasting

confidence: 97%

Addition of zinc to human ejaculate prior to cryopreservation prevents freeze-thaw-induced DNA damage and preserves sperm function

Kotdawala

Kumar

Salian

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To study the effect of addition of zinc to human semen sample prior to cryopreservation on post-thaw sperm quality and function. Methods Semen samples were collected from men attending university infertility clinic for semen analysis (n0109). Liquefied semen samples were cryopreserved in glycerol-egg yolkcitrate medium with or without the prior addition of zinc (100 μM) and stored in liquid nitrogen. After 10 days, the semen samples were thawed to assess the outcome. Sperm motility, DNA integrity, mitochondrial potential and the ability of spermatozoa to undergo capacitation and acrosome reaction was assessed in post-thaw samples. Results Semen samples cryopreserved after addition of zinc had a significantly higher percentage of sperm with intact DNA (p<0.001), mitochondrial function (p<0.001) and progressive motility (p<0.01) compared to the semen samples cryopreserved without zinc supplementation. Apart from this, ability to undergo capacitation and acrosome reaction in vitro was significantly higher in semen samples cryopreserved with zinc (p<0.0001 and p<0.001 respectively).Conclusions Addition of zinc to semen samples prior to cryopreservation helps in preventing the freeze-thaw-induced sperm DNA damage and loss of sperm function.

show abstract

Section: Discussioncontrasting

confidence: 97%

Addition of zinc to human ejaculate prior to cryopreservation prevents freeze-thaw-induced DNA damage and preserves sperm function

Kotdawala

Kumar

Salian

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…In a study by Lewis-Jones et al (1996) 1178 patients referred for fertility treatment had zinc and fructose concentrations in seminal plasma measured, but no statistically significant correlation was found. Danscher et al (1978) have reported that high zinc concentrations are correlated with depressed sperm motility; however, to the contrary, Stankovic and Mikac-Devic (1976) found that a high zinc content in seminal plasma was correlated with high sperm cell motility.…”

Section: Discussionmentioning

confidence: 89%

Histochemical tracing of zinc ions in the rat testis

Sörensen

Stoltenberg²,

Henriksén³

et al. 1998

Molecular Human Reproduction

View full text Add to dashboard Cite

To detect free zinc ions in the rat testes four rats were transcardially perfused with Na 2 S, and the seminiferous tubules from two other rats were incubated in Na 2 S. Sections from the two sources were autometallographically (AMG) developed, whereby zinc sulphide crystal lattices created in the tissue by the sulphide treatment were silver enhanced. Light microscopical analysis showed zinc ions in primary spermatogonia until the zygotene primary spermatocytes (stage I), in late pachytene spermatocytes (stages XII and XIII), and in late spermatids from step 15 to step 19 (stages I-VIII). The highest intensity of AMG grains was detected in the residual bodies and tails of step 19 spermatids. Grains were occasionally found in the cytoplasm of Leydig cells. Sections from animals treated with the chelator diethyldithiocarbamate prior to sulphide treatment showed a complete lack of AMG staining. At ultrastructural levels the AMG grains were found in smooth-surfaced endoplasmic reticulum of all spermatogonial stages, and in the acrosome, midpiece, and tail of late spermatids. The presence of zinc ions in preleptotene spermatocytes and cytoplasmic lobes of late spermatids suggests a specific role of free zinc at the onset of meiosis and at spermiation.

show abstract

“…Abou-Shakra et al, 1989 Arver and Sjoberg, 1982Carpino and Siciliano, 1998Colleen et al, 1975Cooper et al, 199117.2 16.5 14 16 11.76 Hirsch et al, 1991Homonnai et al, 1978Jeyendran et al, 1989Kavanagh, 1985Lewis-Jones et al, 1996 8.4 14 15.0 Mandal and Bhattacharyya, 1986Mandal and Bhattacharyya, 1987aMandal and Bhattacharyya, 1990Mann and Lutwak-Mann, 1981Marmar et al, 1975; atomic absorption 18.9 13.4 9.26 19.0 16.6 Marmar et al, 1975; neutron activation Mawson and Fischer, 1956Mendiratta et al, 1980Papadimas et al, 1983Paz et al, 197714.64 14.7 69.29 10.6 19.5 Ponchietti et al, 1984Rosecrans et al, 1987Schoenfeld et al, 1979Sorensen et al, 1999Stankovic and Mikac-Devic, 197613 12.4 14.4 Stegmayr et al, 1982Umeyama et al, 1986Wood et al, 1982 Makler et al, 1981Mandal and Bhattacharyya, 1987bPolak and Daunter, 1984Velazquez et al, 1977 Purvis et al, 1986Rosecrans et al, 1987Schirren et al, 1977Schoenfeld et al, 1979225 302 136 Sheth and Rao, 1962bTauber et al, 1975…”

Section: Viscositymentioning

confidence: 99%

A Review of the Physical and Chemical Properties of Human Semen and the Formulation of a Semen Simulant

Owen

Katz

2005

Journal of Andrology

349

298

View full text Add to dashboard Cite

A fluid medium was developed to simulate the salient physical and chemical properties of human semen. The composition of the medium was based upon an extensive review of the literature on constituents of human semen. In choosing the ingredients for this medium, the goal was to emphasize properties that influence interactions of human semen with topical contraceptive, prophylactic, or therapeutic products. Among these properties, pH and buffering capacity, osmolarity, ionic strength, and rheological properties play dominant roles in the physico-chemical processes that govern drug release kinetics and delivery vehicle distribution. W hen therapeutic, contraceptive, or prophylactic formulations are applied to the vagina, they encounter a variety of fluids with widely varying physical and chemical properties. These fluids include those that originate in the vagina and those that flow into it (eg, cervical mucus and semen). The fluid actually present at any location within the vagina is a mixture, to a varying extent, of these fluids. The flow, retention, drug delivery kinetics, and bioactivity of vaginal formulations depend upon their interactions with these resident fluids. Work done by our group has determined that the physical and chemical properties of both the delivery vehicle and the surrounding environment are important factors in determining product performance (Katz et al, 1998;Owen et al, 1999aOwen et al, , 2000Owen et al, , 2001Owen et al, , 2003. Understanding of these interactions can, therefore, aid in the design and development of new and improved formulations.One component of such research is the in vitro testing of formulations with fluids representative of those that will be encountered within the vagina. Our laboratory has been developing and applying in vitro assays that focus on how the deployment and delivery of contraceptive and prophylactic compounds are affected by the properties of the delivery vehicle and its interactions with the surrounding fluids. To develop these assays using standardized materials of sufficient volume, we have found it useful to employ simulants of ambient biological fluids. Our for- mulation of a vaginal fluid simulant has been described in a previous publication (Owen and Katz, 1999b), and our semen simulant (and earlier versions) was presented in a number of studies of contraceptive and microbicidal gels (Owen et al, 2003(Owen et al, , 2004Geonnotti and Katz, 2004;Geonnotti et al, 2005a;Geonnotti et al, 2005b). Here we describe the formulation of a semen simulant embodying salient physical and chemical components and properties of human semen. This is based on a comprehensive review of the literature, and we present here, as well, an updated summary of the constituents of human semen. Materials and MethodsThe quantity and composition of human semen have been studied for a variety of reasons (eg, for the diagnosis of conditions such as prostatitis, infertility, and cancer), and the results of these studies were used in the development of our simulant. It i...

show abstract

Zinc and copper in human semen

Cited by 52 publications

References 10 publications

Addition of zinc to human ejaculate prior to cryopreservation prevents freeze-thaw-induced DNA damage and preserves sperm function

Addition of zinc to human ejaculate prior to cryopreservation prevents freeze-thaw-induced DNA damage and preserves sperm function

Histochemical tracing of zinc ions in the rat testis

A Review of the Physical and Chemical Properties of Human Semen and the Formulation of a Semen Simulant

Contact Info

Product

Resources

About