Zinc and Cadmium Toxicity Using a Biotest with Artemia franciscana

Nováková, J.; Danova, D.; Strišková, K.; Hromada, R.; Mičková, Helena; Rabišková, Miloslava

doi:10.2754/avb200776040635

Cited by 9 publications

(3 citation statements)

References 7 publications

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“…Alternative model systems are used in biomedical research (Dvořák and Beňová 2002;Sklenář et al 2006;Nováková et al 2007) in order to protect higher vertebrates. Our experiments have not proved any microbial contamination of fish musculature even 28 days after the intravital exposure of specimens to the ionizing radiation dose of 30 Gy.…”

Section: Discussionmentioning

confidence: 99%

Effects of Gamma-Irradiation on Microbial Contamination and on Histological Changes of Muscle in Poecilia reticulata

et al. 2009

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This study describes the effect of gamma-irradiation on the microbial skin and organ contamination and on the histological changes of fish skeletal musculature. The aquarium fish Poecilia reticulata were exposed to the radiation effect of 60 Co at the doses of 20 and 30 Gy (11.36 Gy/min). Frequent intravital haemorrhages were observed from day 8 after the exposure. A general long-term body fading appeared from day 10 to day 30 of the experiment. Microbiological and histological examinations were done on days 7, 14, 21 and 28. Individual muscle cells were bound together into fascicles surrounded by connective tissue. No significant morphological changes were observed on skeletal muscle cells, except for more watery connective tissue.The presence of Escherichia coli and Aeromonas hydrophila was proved in the body surface samples and in aquarium water based on the microbiological test. A moderate increase of the number of E. coli and A. hydrophila was observed in the intestines in both exposed groups (20 and 30 Gy) depending on the time that elapsed from the exposure (from 1 × 10 1 to 6 × 10 3 CFU). However, the heart, liver and musculature remained sterile. No microbial contamination of musculature was found for the intravital ionizing radiation dose of 30 Gy even for the single specimen killed on day 28 from the exposure.

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Section: Discussionmentioning

confidence: 99%

Effects of Gamma-Irradiation on Microbial Contamination and on Histological Changes of Muscle in Poecilia reticulata

et al. 2009

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“…With rising environmental pollution the interest arises in the consequences of the action of xenobiotics, including hazardous chemical elements, on live organisms (Nováková et al 2007;Čelechovská et al 2008). A series of trials were designed to evaluate the responses of tissues and organs of animal species of economic importance, such as the rabbit, to trace elements (cadmium, lead, mercury) at concentrations that could potentially exist in the feeds on a farm.…”

Section: Xcelligence System Nephrotoxicity Cytotoxicity Lactate Dementioning

confidence: 99%

Real-time monitoring of cadmium toxicity in rabbit kidney cells

et al. 2015

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The aim of this study was to investigate the toxic effect of the metal salt cadmium chloride dihydrate on the rabbit kidney cell line using the xCELLigence system or real-time cell analyser (RTCA), and to compare this relatively new method with standard biological cytotoxicity assays. This system provides real-time monitoring of cell behaviour and proliferative activity during the whole time of experiment. Moreover, after 24 h exposure of cells to cadmium, colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5-difenyl tetrazolium bromide (MTT) test was used to measure the metabolic activity and cytotoxicity was determined by measurement of lactate dehydrogenase (LDH) leaked from damaged cells. We found that renal cells exposed to lower concentrations (5-10 mg·l -1 ) of cadmium tend to grow similarly to control cells, however, cell index was significantly different (P < 0.05) after 24 h. With increasing concentration of cadmium (15-50 mg·l -1 ) significantly lower proliferative (P < 0.05) and metabolic activity (P < 0.05) of cells was observed and cytotoxicity increased simultaneously (P < 0.001). In addition, we found that the real-time monitoring of the cell response was significantly correlated with commonly used biological methods for toxicity measurement, for MTT assay R 2 was 0.9448 (P < 0.01) and for LDH assay R 2 was 0.9466 (P < 0.01), respectively. The present study is the first report when combination of RTCA, MTT assay and LDH test was used for cadmium nephrotoxicity assessment. In all these methods, the toxic effect of cadmium on rabbit kidney cells increased in a concentration-dependent manner. xCELLigence system, nephrotoxicity, cytotoxicity, lactate dehydrogenaseIncreased industrial activities brought gradual redistribution of a number of toxic elements from the terrestrial crust into the environment and thus increased the potential exposure of humans and animals. With rising environmental pollution the interest arises in the consequences of the action of xenobiotics, including hazardous chemical elements, on live organisms (Nováková et al. 2007;Čelechovská et al. 2008). A series of trials were designed to evaluate the responses of tissues and organs of animal species of economic importance, such as the rabbit, to trace elements (cadmium, lead, mercury) at concentrations that could potentially exist in the feeds on a farm. The daily rations included carrots, potatoes, or beetroot. Cadmium belongs to heavy metals widely distributed in the environment, obtained as a by-product of refining. Its compounds are used in the electroplating of metals, alkaline batteries or in compounds with other metals. Relatively high quantities of cadmium are present in phosphate fertilisers (from some locations), which increase the concentration of cadmium in soil and plants. Cadmium is also highly toxic to organisms living in the aqueous environment (Koréneková et al. 2002). Because of its ability to reabsorb and accumulate divalent metals, kidney is the first target organ of heavy metal toxicity. The extent of renal da...

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“…The environment including the aquatic ecosystems is often contaminated with various exogenous agents at low concentrations (Beňová et al 2007;Nováková et al 2007). Although the response of many test organisms to various agents is well-known, our knowledge of the toxicity of mixtures of such agents is rather limited.…”

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confidence: 99%

Use of the Crustacean Artemia franciscana for Alternative Biotests

et al. 2010

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Use of the crustacean Artemia franciscana for alternative biotests of the second generation was studied, and possible experimental design and applications of such tests outlined. In addition to the classical use in ecotoxicology, the test can be used in pharmacology as well, or to monitor the effects of ionizing radiation in co-exposure with some chemical compounds. The synergistic effect of co-exposure of PCB (DELOR 103), cadmium chloride and potassium dichromate with beta 89 Sr irradiation was shown. We also demonstrated the anti-oxidative and pro-oxidative effects of the ascorbic acid in dependence on its concentration. Use of the pharmaco-toxicological screening in search for the novel inhibitors of cyclin-dependent kinases was demonstrated as well, showing that Artemia franciscana may be used as a suitable biosensor instead of the expensive tests on higher vertebrates.

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Zinc and Cadmium Toxicity Using a Biotest with Artemia franciscana

Cited by 9 publications

References 7 publications

Effects of Gamma-Irradiation on Microbial Contamination and on Histological Changes of Muscle in Poecilia reticulata

Effects of Gamma-Irradiation on Microbial Contamination and on Histological Changes of Muscle in Poecilia reticulata

Real-time monitoring of cadmium toxicity in rabbit kidney cells

Use of the Crustacean Artemia franciscana for Alternative Biotests

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