Zinc alpha2 glycoprotein alleviates palmitic acid-induced intracellular lipid accumulation in hepatocytes

Xiao, Xinhua; Han, Li; Qi, Xiaoyan; Wang, Yadi; Xu, Chenghai; Liu, Gexin; Ge, Wen; Liu, Jianghua

doi:10.1016/j.mce.2016.06.003

Cited by 37 publications

(32 citation statements)

References 40 publications

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“…Lpin1 and GPAM, genes of lipid metabolism involved in the metabolization-related process of glycerides, were significantly upregulated in the ZAG knockout group, which may influence the lipid metabolism in adipose tissue. Studies have showed that in vitro experimental the lipid synthesis capacity of cells was significantly enhanced after ZAG interference [14], the deletion of ZAG in our results changed the expression of lipid metabolism genes. Our results validate the function of ZAG in fat metabolism and provide theoretical support for better studies on lipid metabolism.…”

Section: Discussionsupporting

confidence: 45%

“…Dexamethasone (DEX) is an analogue of glucocorticoid, which can be used to simulate stress response [11,12]. Many studies have shown that DEX treatment can disrupt the body's lipid metabolism [13,14], and the treatment of DEX can accelerate lipid decomposition [15]. Exogenous glucocorticoid treatment or analogue of glucocorticoid have been shown to stimulate both triglyceride synthesis and lipolysis in white adipose tissue [16], which caused lipid metabolism disorder.…”

Section: Introductionmentioning

confidence: 99%

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Zinc-α2-Glycoprotein Knockout Influenced Genes Expression Profile in Adipose Tissue and Decreased the Lipid Mobilizing After Dexamethasone Treatment in Mice

Wen-ge

Qiao

et al. 2020

Horm Metab Res

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Zinc-α2-glycoprotein (ZAG), as an adipokine, plays an important role in lipid metabolism. However, its influence on whole gene expression profile in adipose tissue is not known. Under stress condition, how ZAG affects the lipid metabolism is also unclear. Therefore, in this study ZAG systemic knockout (KO) mice were used as a model to reveal the genes expression profile in visceral fat tissues of ZAG KO mice and wild-type mice by genome-wide microarray screening. Then dexamethasone (DEX) was used to explore the effect of ZAG deletion on body fat metabolism under stress. Our results showed that 179 genes were differentially expressed more than 1.5 times between ZAG KO mice and wild type mice, of which 26 genes were upregulated dramatically and 153 genes were significantly downregulated. Under DEX simulated stress, ZAG systemic knockout in vivo resulted in a markedly decrease of triglycerides (TG) and nonesterified fatty acid (NEFA) content in in plasma. Similarly, for lipid catabolism, ZAG KO led to a significant increase of phosphorylated HSL (p-HSL) protein and a rising tendency of adipose triglyceride lipase (ATGL) protein relative to those of the DEX group. For lipid anabolism, fatty acid synthase (FAS) and adiponectin protein expression in visceral fat rose notably in ZAG KO mice after DEX treatment. In conclusion, ZAG knockout can affect the gene expression profile of adipose tissue, reduce elevated TG and NEFA levels in plasma, and alter lipid metabolism under DEX treatment. These findings provide new insights into the mechanism of lipid metabolic disorders in response to stress.

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Section: Discussionsupporting

confidence: 45%

Section: Introductionmentioning

confidence: 99%

Zinc-α2-Glycoprotein Knockout Influenced Genes Expression Profile in Adipose Tissue and Decreased the Lipid Mobilizing After Dexamethasone Treatment in Mice

Wen-ge

Qiao

et al. 2020

Horm Metab Res

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“…ZAG was initially isolated from human plasma [31] and was subsequently proved to be a novel adipokine that can be secreted by adipose tissue and adipocytes [19]. Basic experiments have verified that ZAG could inhibit lipogenesis [21,32], stimulate lipolysis [21,32,33] and β-oxidation [32,33], promote white adipose tissue browning [33,34], and thus play a critical role in regulating body weight. Recent clinical studies also found that serum ZAG levels were significantly lower in overweight/obese patients and were negatively correlated with BMI, waist circumstance, hip circumference, and fat mass [21,22].…”

Section: Discussionmentioning

confidence: 99%

Serum Zinc-α2-Glycoprotein Levels in Patients with or without Coronary Artery Disease in Chinese North Population

Liu

Zhu

et al. 2020

International Journal of Endocrinology

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Coronary artery disease (CAD), the leading cause of morbidity and mortality, has imposed huge health and economic burdens globally. Zinc-α2-glycoprotein (ZAG) is a novel adipokine. Increasing evidence suggests the close relationship between serum ZAG levels and various cardiometabolic risk factors. However, the relationship between serum ZAG levels and CAD is still not fully clarified. We conducted this study to evaluate serum ZAG levels and its association with cardiovascular risk factors. A total of 129 patients with CAD, 99 patients with noncoronary artery disease (NCAD), and 121 controls were recruited in this retrospective study. CAD (coronary artery stenosis ≥50%) or NCAD (coronary artery stenosis <50%) patients who underwent coronary angiography were diagnosed according to the American Heart Association criteria. Serum ZAG levels were determined via commercial enzyme-linked immunosorbent assay (ELISA) kits. e results showed that serum ZAG levels in CAD and NCAD groups were significantly decreased when compared with those in the control group. Multiple stepwise regression analysis revealed that the grouping variable (control, NCAD, and CAD) was an independent determinant of serum ZAG levels (β � − 0.328, P < 0.001) after controlling other confounding factors. Further multivariate ordinary logistic regression analysis demonstrated that the risk of grouping at one level higher in subjects with the lowest tertile of ZAG levels was 2.28-fold higher than those with the highest tertile levels (OR � 3.281, 95% CI 1.782-6.038, P < 0.001). e receiver-operating characteristic (ROC) curve analysis showed that serum ZAG could distinguish CAD patients (AUC � 0.706, 95% CI, 0.643-0.770, P < 0.05), NCAD patients (AUC � 0.673, 95% CI, 0.602-0.743, P < 0.05), and NCAD and CAD patients (AUC � 0.692, 95% CI, 0.633-0.750, P < 0.05) from controls. In conclusion, serum ZAG levels were significantly decreased in NCAD/CAD patients. e decreased serum ZAG levels were independently associated with the presence of NCAD/CAD. ZAG might serve as a candidate diagnostic biomarker for NCAD/CAD.

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“…In addition, ZAG inhibits the activity of several key enzymes in the lipogenesis pathway that may contribute to the development of cachexia. 31 , 32 …”

Section: Discussionmentioning

confidence: 99%

Increased serum levels of S100A1, ZAG, and adiponectin in cachectic patients with COPD

Mokari-Yamchi

Sharifi

Kheirouri

2018

COPD

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BackgroundCOPD is a common irreversible obstructive airway disease. S100A1, ZAG, and adiponectin are important regulators of energy metabolism and body weight. Therefore, the aim of this study was to assess resting metabolic rate (RMR) and its association with serum levels of S100A1, ZAG, and adiponectin in cachectic and noncachectic COPD patients.Patients and methodsNinety men with COPD, aged 40–70 years, were enrolled in the study. Patients were divided into the following two groups based on the unintentional weight loss of .7.5% in previous 6 months: noncachectic (n=45) and cachectic (n=45). The groups were matched based on age and body mass index (BMI). RMR was measured by indirect calorimetry method. Anthropometric indices and body composition were also measured. Serum levels of S100A1, ZAG, and adiponectin were measured by ELISA.ResultsCachectic patients had significantly higher RMR than controls (P<0.001). Serum levels of ZAG, S100A1, and adiponectin were significantly higher in the cachexia group (P<0.0001). RMR was not significantly associated with S100A1, ZAG, and adiponectin levels. However, weight loss of patients was significantly associated with serum levels of ZAG and adiponectin (both, β=0.22, P=0.03). Strong and positive association were found between the serum levels of S100A1 and ZAG (β=0.88, P<0.0001), S100A1 and adiponectin (β=0.86, P<0.0001), and also ZAG and adiponectin (β=0.83, P<0.0001).ConclusionThe potential role of these factors in the wasting process is considerable. Also, the association between serum levels of S100A1, ZAG, and adiponectin represents that these three proteins are probably related to specific functions.

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Zinc alpha2 glycoprotein alleviates palmitic acid-induced intracellular lipid accumulation in hepatocytes

Cited by 37 publications

References 40 publications

Zinc-α2-Glycoprotein Knockout Influenced Genes Expression Profile in Adipose Tissue and Decreased the Lipid Mobilizing After Dexamethasone Treatment in Mice

Zinc-α2-Glycoprotein Knockout Influenced Genes Expression Profile in Adipose Tissue and Decreased the Lipid Mobilizing After Dexamethasone Treatment in Mice

Serum Zinc-α2-Glycoprotein Levels in Patients with or without Coronary Artery Disease in Chinese North Population

Increased serum levels of S100A1, ZAG, and adiponectin in cachectic patients with COPD

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