Zinc accumulation and vegetation ecology in the allotetraploid, <i>Arabidopsis kamchatica</i> ssp. <i>kawasakiana</i>

Abstract

Arabidopsis kamchatica ssp. kawasakiana, a member of the family Brassicaceae, is an endangered winter annual species that grows on sandy coasts and lakesides. A. kamchatica is an allotetraploid plant produced by the hybridization of two closely related diploid taxa, the Zn/Cd hyperaccumulator A. halleri and the non-accumulator A. lyrata. The heavy metal accumulation and vegetation ecology of A. k. ssp. kawasakiana were investigated by collecting leaves and rhizosphere soil samples in three natural habitats on the shore of Lake Biwa in Japan. Leaf Zn contents in almost all plants were above the level required by hyperaccumulators. Plants from one habitat preferred to grow on soils with topical high Zn levels, whereas rhizosphere soils from other populations contained basal levels of Zn suggesting that plant vegetation is more affected by soil disturbance and by soil Zn contents. A. k. ssp. kawasakiana plants were also found to be tolerant to Zn and Cd, in contrast to the nontolerant species A. thaliana. These findings indicated that A. k. ssp. kawasakiana is a facultative Zn hyperaccumulator, inheriting the trait from its parent A. halleri. Furthermore, A. k. ssp. kawasakiana is a self-compatible plant and Arabidopsis floral dip transformation might be applicable to this plant. Considering their natural diversity, A. k. ssp. kawasakiana will help the determination of the molecular mechanisms by which plants accumulate and tolerate heavy metals.

Metal accumulation and its effect on leaf herbivory in an allopolyploid species Arabidopsis kamchatica inherited from a diploid hyperaccumulator A. halleri

“…Furthermore, genetic variation in ability to accumulate metal among populations should exist in A. kamchatica , as already found in A. halleri (Babst‐Kostecka et al, 2018; Macnair, 2002; Stein et al, 2017). Actually, large variation in Zn accumulation among several natural populations of A. kamchatica has been reported from Japan, which included the locality of our kam2 population's origin (Kosugi, Nishizawa, Kawabe, & Harada, 2016). Our study using two A. kamchatica genotypes also detected a variation in accumulation level.…”