ZIKV-Specific NS1 Epitopes as Serological Markers of Acute Zika Virus Infection

Kam, Yiu‐Wing; Leite, Juliana Almeida; Amrun, Siti Naqiah; Lum, Fok‐Moon; Yee, Wearn-Xin; Bakar, Farhana Abu; Eng, Kai Er; Lye, David C.; Leo, Yee‐Sin; Chong, Chia-Yin; Freitas, André Ricardo Ribas; Milanez, Guilherme Paier; Proença-Módena, José Luiz; Rénia, Laurent; Costa, Fabio T. M.; Ng, Lisa F. P.

doi:10.1093/infdis/jiz092

Cited by 16 publications

(38 citation statements)

References 39 publications

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“…Interestingly, peptide 32 (amino acid residues 453-470) maps to a region that overlaps with a DENV-2 epitope (amino acid residues 451-468) described for immune sera of DENV-2-infected patients. 27 Identified putative epitopes were preliminary diagnostically evaluated with 38 patient samples. 23,38 Since they share low sequence identity (43.75%), this epitope could be conformationally different and thus differentially recognised by ZIKV-and DENV-specific antibodies.…”

Section: Discussionmentioning

confidence: 99%

“…18,[24][25][26][27] Twenty-two donors who had antibody levels lower than the assigned cut-off (mean + SD) in all three viruses (Supplementary figure 1a, b) were used as the healthy control pool and set as a baseline reference. 18,[24][25][26][27] Twenty-two donors who had antibody levels lower than the assigned cut-off (mean + SD) in all three viruses (Supplementary figure 1a, b) were used as the healthy control pool and set as a baseline reference.…”

Section: Zikv Patients Produce a Robust And Protective Humoral Responsementioning

confidence: 99%

“…24,25,27 Peptides were generated as a ZIKV and DENV peptide pair of corresponding sequences. 24,25,27 Peptides were generated as a ZIKV and DENV peptide pair of corresponding sequences.…”

Section: Linear Peptide Librariesmentioning

confidence: 99%

“…24,25,27 Briefly, streptavidincoated plates (Thermo Fisher Scientific) were blocked with 0.1% PBST (0.1% Tween-20 in PBS) containing 1% sodium caseinate (Sigma-Aldrich) and 1% bovine serum albumin (BSA; Sigma-Aldrich) overnight at 4°C, before addition of biotinylated peptides (1:1000 dilution in 0.1% PBST), followed by heat-inactivated pooled healthy control and patient plasma/serum samples (1:2000 dilution in 0.1% PBST). 24,25,27 Briefly, streptavidincoated plates (Thermo Fisher Scientific) were blocked with 0.1% PBST (0.1% Tween-20 in PBS) containing 1% sodium caseinate (Sigma-Aldrich) and 1% bovine serum albumin (BSA; Sigma-Aldrich) overnight at 4°C, before addition of biotinylated peptides (1:1000 dilution in 0.1% PBST), followed by heat-inactivated pooled healthy control and patient plasma/serum samples (1:2000 dilution in 0.1% PBST).…”

Section: Peptide-based Elisamentioning

confidence: 99%

“…Reactions were developed using TMB Stop reagent (Sigma-Aldrich), and absorbance was measured at 450 nm in a microplate autoreader (Tecan, M€ annedorf, Z€ urich, Switzerland) 18,[24][25][26][27]. Reactions were developed using TMB Stop reagent (Sigma-Aldrich), and absorbance was measured at 450 nm in a microplate autoreader (Tecan, M€ annedorf, Z€ urich, Switzerland) 18,[24][25][26][27].…”

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confidence: 99%

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Novel differential linear B‐cell epitopes to identify Zika and dengue virus infections in patients

et al. 2019

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Objectives Recent Zika virus (ZIKV) outbreaks challenged existing laboratory diagnostic standards, especially for serology‐based methods. Because of the genetic and structural similarity of ZIKV with other flaviviruses, this results in cross‐reactive antibodies, which confounds serological interpretations. Methods Plasma from Singapore ZIKV patients was screened longitudinally for antibody responses and neutralising capacities against ZIKV. Samples from healthy controls, ZIKV patients and DENV patients were further assessed using ZIKV and DENV peptides of precursor membrane (prM), envelope (E) or non‐structural 1 (NS1) viral proteins in a peptide‐based ELISA for epitope identification. Identified epitopes were re‐validated and diagnostically evaluated using sera of patients with DENV, bacteria or unknown infections from Thailand. Results Long‐lasting ZIKV‐neutralising antibodies were elicited during ZIKV infection. Thirteen potential linear B‐cell epitopes were identified, and of these, four common flavivirus, three ZIKV‐specific and one DENV‐specific differential epitopes had more than 50% sensitivity and specificity. Notably, ZIKV‐specific peptide 26 on domain I/II of E protein (amino acid residues 271–288) presented 80% sensitivity and 85.7% specificity. Importantly, the differential epitopes also showed significance in differentiating non‐flavivirus patient samples. Conclusion Linear B‐cell epitope candidates to differentiate between ZIKV and DENV infections were identified, providing the first step towards the design of a much‐needed serology‐based assay.

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Section: Discussionmentioning

confidence: 99%

Section: Zikv Patients Produce a Robust And Protective Humoral Responsementioning

confidence: 99%

“…24,25,27 Peptides were generated as a ZIKV and DENV peptide pair of corresponding sequences. 24,25,27 Peptides were generated as a ZIKV and DENV peptide pair of corresponding sequences.…”

Section: Linear Peptide Librariesmentioning

confidence: 99%

Section: Peptide-based Elisamentioning

confidence: 99%

mentioning

confidence: 99%

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Novel differential linear B‐cell epitopes to identify Zika and dengue virus infections in patients

et al. 2019

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Modulation of cellular machineries by Zika virus‐encoded proteins

Dong

Xiao

Liang

2022

Journal of Medical Virology

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The strain of Zika virus (ZIKV) that circulated during the 2015 epidemic in Brazil has been associated with more than 2000 cases of microcephaly from September 2015 through November 2016. The viral genome determines the biology and pathogenesis of a virus and the virus employs its own gene products to evade host immune surveillance, manipulate cellular machineries, and establish efficient replication. Therefore, understanding the functions of virus‐encoded protein not only aids the knowledge of ZIKV biology but also guides the development of anti‐ZIKV drugs. In this review, we focus on 10 proteins encoded by ZIKV and summarize their functions in ZIKV replication and pathogenesis according to studies published in the past 6 years.

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