Zika virus leads to olfactory disorders in mice by targeting olfactory ensheathing cells

Zhou, Jia; Guan, Mengyue; Li, Rui-Ting; Qi, Yini; Yang, Guan; Deng, Yong-Qiang; Li, Xiaofeng; Li, Liang; Yang, Xiao; Liu, Jian-Feng; Qin, Cheng‐Feng

doi:10.1016/j.ebiom.2023.104457

Cited by 7 publications

(5 citation statements)

References 77 publications

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“…The onset of SARS-CoV-2 infection initiates with the attachment of the viral glycoprotein spike S protein to angiotensin-converting enzyme 2 (ACE2), a receptor belonging to the human olfactory epithelium (OE). It is widely recognized that viral proteomes consist of various arrangements of protein–protein interactions (PPIs) to fulfill a wide range of essential functions throughout the viral life cycle [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Proteoform Analysis of the Human Olfactory System: A Window into Neurodegenerative Diseases

Rusi,

Pennacchia,

Ruqa

et al. 2024

Proteomes

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Background: Very little is known about the proteome of the human olfactory system and how diseases associated with olfactory dysfunctions can affect it. With this review, we try to summarize the existing literature on the use of this technique for a better understanding of the neurodegenerative disease process. Methods: We used the PubMed database and found different articles which were then selected independently by three authors. Results: We found 157 articles, of which, after careful selection, only 30 were analyzed in this review. We presented all the associations identified between the protein/pathway alterations neurodegenerative diseases and SARS-CoV-2 infection. Conclusions: We think that the proteome of the olfactory system through blood, saliva, and mucus analysis could be a new way to better understand, diagnose, and finally treat neurodegenerative diseases.

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Section: Resultsmentioning

confidence: 99%

Proteoform Analysis of the Human Olfactory System: A Window into Neurodegenerative Diseases

Rusi,

Pennacchia,

Ruqa

et al. 2024

Proteomes

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“…Understanding clinical and laboratory findings can aid in narrowing potential routes of interest. In recent findings, ZIKV has been discovered in saliva, urine, body fluids, breast milk, semen, and in nasopharyngeal swabs [ 9 , 10 ]. Sustained secretion of Zika in lacrimal fluids and in nasal mucosa suggests that close contact with an individual or exposure to the infectious body fluids could transmit and cause infection in others.…”

Section: Discussionmentioning

confidence: 99%

“…Other methods of transmission include congenital, sexual, and intranasal transmission. Clinicians have reported an array of symptoms, including speech delay, intellectual disability, movement issues, and vision and hearing loss [ 9 ]. Due to these congenital clinical findings, a new term was coined: congenital Zika syndrome (CZS).…”

Section: Introductionmentioning

confidence: 99%

Intranasal Immunization for Zika in a Pre-Clinical Model

Shah,

Patel,

Bagwe

et al. 2024

Viruses

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Humans continue to be at risk from the Zika virus. Although there have been significant research advancements regarding Zika, the absence of a vaccine or approved treatment poses further challenges for healthcare providers. In this study, we developed a microparticulate Zika vaccine using an inactivated whole Zika virus as the antigen that can be administered pain-free via intranasal (IN) immunization. These microparticles (MP) were formulated using a double emulsion method developed by our lab. We explored a prime dose and two-booster-dose vaccination strategy using MPL-A® and Alhydrogel® as adjuvants to further stimulate the immune response. MPL-A® induces a Th1-mediated immune response and Alhydrogel® (alum) induces a Th2-mediated immune response. There was a high recovery yield of MPs, less than 5 µm in size, and particle charge of −19.42 ± 0.66 mV. IN immunization of Zika MP vaccine and the adjuvanted Zika MP vaccine showed a robust humoral response as indicated by several antibodies (IgA, IgM, and IgG) and several IgG subtypes (IgG1, IgG2a, and IgG3). Vaccine MP elicited a balance Th1- and Th2-mediated immune response. Immune organs, such as the spleen and lymph nodes, exhibited a significant increase in CD4+ helper and CD8+ cytotoxic T-cell cellular response in both vaccine groups. Zika MP vaccine and adjuvanted Zika MP vaccine displayed a robust memory response (CD27 and CD45R) in the spleen and lymph nodes. Adjuvanted vaccine-induced higher Zika-specific intracellular cytokines than the unadjuvanted vaccine. Our results suggest that more than one dose or multiple doses may be necessary to achieve necessary immunological responses. Compared to unvaccinated mice, the Zika vaccine MP and adjuvanted MP vaccine when administered via intranasal route demonstrated robust humoral, cellular, and memory responses. In this pre-clinical study, we established a pain-free microparticulate Zika vaccine that produced a significant immune response when administered intranasally.

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“…Two other attractive candidates for further studies are the genes DLX5 and ATOH8, which are also repressed in ZIKV-infected cells. DLX5 has been shown to be involved in the development of the olfactory system [ 36 ], and ZIKV has recently been associated with olfactory disorders in mice [ 37 ] as well as reduced olfactory function in patients with ZIKV-induced Guillain-Barré syndrome [ 38 ]. ATOH8 is involved in the developing nervous system as well as the inner ear [ 39 ], and ZIKV has been observed to cause hearing loss [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Kunjin Virus, Zika Virus, and Yellow Fever Virus Infections Have Distinct Effects on the Coding Transcriptome and Proteome of Brain-Derived U87 Cells

Brand

Deschamps-Francoeur

Bullard-Feibelman

et al. 2023

Viruses

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As obligate intracellular parasites, viruses rely heavily on host cells for replication, and therefore dysregulate several cellular processes for their benefit. In return, host cells activate multiple signaling pathways to limit viral replication and eradicate viruses. The present study explores the complex interplay between viruses and host cells through next generation RNA sequencing as well as mass spectrometry (SILAC). Both the coding transcriptome and the proteome of human brain-derived U87 cells infected with Kunjin virus, Zika virus, or Yellow Fever virus were compared to the transcriptome and the proteome of mock-infected cells. Changes in the abundance of several hundred mRNAs and proteins were found in each infection. Moreover, the alternative splicing of hundreds of mRNAs was found to be modulated upon viral infection. Interestingly, a significant disconnect between the changes in the transcriptome and those in the proteome of infected cells was observed. These findings provide a global view of the coding transcriptome and the proteome of Flavivirus-infected cells, leading to a better comprehension of Flavivirus–host interactions.

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Zika virus leads to olfactory disorders in mice by targeting olfactory ensheathing cells

Cited by 7 publications

References 77 publications

Proteoform Analysis of the Human Olfactory System: A Window into Neurodegenerative Diseases

Proteoform Analysis of the Human Olfactory System: A Window into Neurodegenerative Diseases

Intranasal Immunization for Zika in a Pre-Clinical Model

Kunjin Virus, Zika Virus, and Yellow Fever Virus Infections Have Distinct Effects on the Coding Transcriptome and Proteome of Brain-Derived U87 Cells

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