Zika virus impairs growth in human neurospheres and brain organoids

Garcez, Patrícia P.; Loiola, Erick Correia; Costa, Rosa Maria Esteves Moreira da; Higa, Luiza M.; Trindade, Pablo; Delvecchio, Rodrigo; Nascimento, Juliana; Brindeiro, Rodrigo M.; Tanuri, Almicar; Rehen, Stevens

doi:10.7287/peerj.preprints.1817v3

Cited by 152 publications

(188 citation statements)

References 4 publications

Supporting

Mentioning

169

Contrasting

Unclassified

Order By: Relevance

“…ZIKV targets human brain cells, reduces their viability and growth. ZIKV abrogates neurogenesis during human brain development . Recently, organoids (three‐dimensional stem cells culture) have been developed to form a model for the human developing brain.…”

Section: Mechanism Of Microcephalymentioning

confidence: 99%

Zika and pregnancy: A comprehensive review

Hajra

Bandyopadhyay

Heise

et al. 2016

American J Rep Immunol

View full text Add to dashboard Cite

Zika virus (ZIKV) infection is a well-nurtured topic for healthcare personnel nowadays.Central nervous system involvement including microcephaly and ocular involvements has already been reported in neonates of affected pregnant ladies. In this article, we have discussed these effects on the newborns of ZIKV-infected mothers. The proposed pathogenesis, modes of transmission of this infection from mothers to the fetuses, diagnosis of the cases and precaution for the pregnant ladies have also been discussed. We have gathered the recently available data on the risk of ZIKV for expectant mothers from PubMed, https://www.gov.uk/guidance/zika-virus as well as from centers for disease control and prevention websites. K E Y W O R D Sfetal anomaly, microcephaly, pregnancy, Zika virus infection

show abstract

Section: Mechanism Of Microcephalymentioning

confidence: 99%

Zika and pregnancy: A comprehensive review

Hajra

Bandyopadhyay

Heise

et al. 2016

American J Rep Immunol

View full text Add to dashboard Cite

show abstract

“…Traditionally, ZIKV was thought to be a mild vector‐borne disease transmitted by mosquitoes, primarily Aedes aegypti or Aedes albopictus . ZIKV induces cell cycle arrest and apoptosis (Garcez et al, ; Li et al, ; Tang et al, ), autophagy (Delvecchio et al, ; Liang et al, ), and differentiation defects in the developing nervous system (Hanners et al, ; Li et al, ). ZIKV infects cells when it interacts with cell surface receptors and is then internalized via endocytosis (Perera‐Lecoin, Meertens, Carnec, & Amara, ).…”

Section: Infectious Agentsmentioning

confidence: 99%

The role of teratogens in neural crest development

Cerrizuela

Vega-Lopez

Aybar

2020

Birth Defects Research

View full text Add to dashboard Cite

The neural crest (NC), discovered by Wilhelm His 150 years ago, gives rise to a multipotent migratory embryonic cell population that generates a remarkably diverse and important array of cell types during the development of the vertebrate embryo. These cells originate in the neural plate border (NPB), which is the ectoderm between the neural plate and the epidermis. They give rise to the neurons and glia of the peripheral nervous system, melanocytes, chondrocytes, smooth muscle cells, odontoblasts and neuroendocrine cells, among others. Neurocristopathies are a class of congenital diseases resulting from the abnormal induction, specification, migration, differentiation or death of NC cells (NCCs) during embryonic development and have an important medical and societal impact. In general, congenital defects affect an appreciable percentage of newborns worldwide. Some of these defects are caused by teratogens, which are agents that negatively impact the formation of tissues and organs during development. In this review, we will discuss the teratogens linked to the development of many birth defects, with a strong focus on those that specifically affect the development of the NC, thereby producing neurocristopathies.Although increasing attention is being paid to the effect of teratogens on embryonic development in general, there is a strong need to critically evaluate the specific role of these agents in NC development. Therefore, increased understanding of the role of these factors in NC development will contribute to the planning of strategies aimed at the prevention and treatment of human neurocristopathies, whose etiology was previously not considered.

show abstract

“…In a similar vein, organoids generated from a patient with autism spectrum disorder (ASD) have allowed researchers to pinpoint a transcription factor (FOXG1), which when overexpressed causes an increase in GABAergic inhibitory neurons . Finally, brain organoids have been used to study the effects of Zika virus infection , which resulted in a 40% growth reduction of the neural tissues. These studies strongly indicate that organoids can enhance our understanding of not only the developmental abnormalities themselves, but also on their possible treatment, even in cases of polygenic disorders.…”

Section: Organoids As Models Of Diseasementioning

confidence: 99%

Dawn of the organoid era

Akkerman

Defize

2017

BioEssays

View full text Add to dashboard Cite

Novel and updated approaches of culturing cells in 3D are rapidly advancing our understanding of development, health, and disease. As tissues have been found to behave more realistically in 3D than in 2D cultures, organoid technology in combination with recent advances in the isolation and generation of stem cells, has rapidly become a promising concept in developmental and regenerative research. The development of all kinds of tissues can now be studied ''in a dish,'' allowing more detailed observations of stem cell maintenance, morphogens, and differentiation. This review explores how organoids have revolutionized academic research over the last 4 decades, and how they may continue to do so. It also addresses remaining hurdles in 3D cell culturing, and how they may be overcome.

show abstract

Zika virus impairs growth in human neurospheres and brain organoids

Abstract: SUMMARY:We provide evidence that Zika virus infects human iPS-derived neural stem cells, causing cell death and reduced growth in neurospheres and cerebral organoids.

Cited by 152 publications

References 4 publications

Zika and pregnancy: A comprehensive review

Zika and pregnancy: A comprehensive review

The role of teratogens in neural crest development

Dawn of the organoid era

Contact Info

Product

Resources

About