Zidovudine Glycosylation by Filamentous Fungi Leads to a Better Redox Stability and Improved Cytotoxicity in B16F10 Murine Melanoma Cells

Arruda, Evilanna Lima; Japiassu, Kamila Bohne; Souza, Paula Letícia de Melo; Araujo, Kelly Carolina Frauzino; Thomaz, Douglas Vieira; Cortez, Alane Pereira; Garcia, Luane Ferreira; Valadares, Marize Campos; Gil, Eric de Souza; Oliveira, Valéria de

doi:10.2174/1871520620666200424112504

Cited by 3 publications

(3 citation statements)

References 22 publications

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“…C. blakesleeana and C. echinulata are two species that are often used in xenobiotic biotransformation studies [ 4 , 10 , 15 ], and both grow as biofilms if cultivated in the same manner as C. elegans , i.e., in an Erlenmeyer flask containing a steel spring. To determine what, if any, potential signalling molecules are produced by these fungi, supernatants from 120 h planktonic and biofilm cultures were extracted with ethyl acetate, and the extracts analysed by GC-MS.…”

Section: Resultsmentioning

confidence: 99%

Regulation of Cunninghamella spp. biofilm growth by tryptophol and tyrosol

Khan

Saleem

Murphy

2021

Biofilm

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Fungi belonging to the genus Cunninghamella are often used as microbial models of mammalian metabolism owing to their ability to transform a range of xenobiotic compounds. Furthermore, under specific growth conditions species such as Cunninghamella elegans and Cunninghamella echinulata grow as biofilms enabling a convenient semi-continuous production of valuable drug metabolites. However, the molecular mechanism of biofilm regulation is not understood, thus controlling biofilm thickness limits the productive applications of it. In this paper we describe the identification of two molecules, tyrosol and tryptophol, that were identified in C. blakesleeana cultures, but not in C. elegans and C. echinulata . The molecules are known quorum sensing molecules (QSMs) in yeast and their potential role in Cunninghamella biofilm regulation was explored. Both were present in higher concentrations in C. blakesleeana planktonic cultures compared with biofilms; they inhibited the growth of the fungus on agar plates and selectively inhibited biofilm growth in liquid cultures. The molecules had a comparatively minor impact on the biofilm growth of C. elegans and C. echinulata and on the growth of these fungi on agar plates. Finally, when exogenous tyrosol or tryptophol was added to previously grown C. blakesleeana biofilm, detachment was visible and new additional planktonic culture was measured, confirming that these molecules specifically regulate biofilm growth in this fungus.

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Section: Resultsmentioning

confidence: 99%

Regulation of Cunninghamella spp. biofilm growth by tryptophol and tyrosol

Khan

Saleem

Murphy

2021

Biofilm

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“…Differential glycosylation patterns on the drug could lead to size and charge heterogeneity and may affect ultimate therapeutic effect. Arruda et al also found that Zidovudine glycosylation patterns affected its efficacy on melanoma [11].…”

Section: Glycosylating Pharmaceuticals For Delivery Improvementmentioning

confidence: 95%

Sweetening the Deal: Glycosylation and its Clinical Applications

Zhang¹,

Sun²

2020

IPJBS

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Glycosylation, an important and nearly ubiquitous form of post-translational modification in eukaryotes, has been studied for its clinical applications. Its complex nature and heterogeneity of functions in the human body has allowed researchers to take multiple approaches in applying it to modern medicine. These approaches include glycosylation of pharmaceuticals to improve delivery; using glycosylation as vaccine/drug targets; editing the glycosylation pattern of immune system components; and profiling glycosylation signatures of diseases for diagnostic tests. In addition, glycosylation has also been used as a tool in developing potential therapeutics for the Covid-19 pandemic. This review covers and summarizes recent, interesting findings associated with the various approaches in the clinical study of glycosylation and aims to inspire future research in this promising direction.

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“…Palavras chaves: Antioxidantes; Quimioinformática; Análise multivariada; Conservantes Farmacêuticos drug, foodstuff and cosmetics (ARRUDA et al, 2020;MOREIRA et al, 2022;NAGARAJAN et al, 2020).…”

Section: Introductionunclassified

Natural or synthetic? Classification of common preservatives in food and drug industry by artificial intelligence

Thomaz¹,

Contardi²,

Santos³

et al. 2022

BJHP

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Monoaromatic antioxidants are one of the major classes of small druggable molecules whose use is widespread as preservatives in pharmaceutical and foodstuff industry. The differentiation of these compounds according to their source is notably difficult due to their shared structural features. This work showcases how to promote the classification of natural and synthetic monoaromatic antioxidants using multivariate analysis, data mining and machine learning algorithms. Physicochemical and biopharmaceutical molecular descriptors were selected and calculated do render alignment and classification models using principal components analysis, data mining, support-vector machines (linear kernel) and multilayer perceptron. We showcased that physicochemical and biopharmaceutical molecular predictors may be suitable attributes for differentiating natural and synthetic monoaromatic antioxidants, since their outputs from multivariate analysis, data mining and machine learning algorithms generated a reliable and accurate model for prompt classification of natural and synthetic monoaromatic antioxidants. Moreover, all classification models yielded accuracies above 80%. This work therefore sheds light on the use of artificial intelligence in the development of classifiers for pharmaceutical and foodstuff applications.

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Zidovudine Glycosylation by Filamentous Fungi Leads to a Better Redox Stability and Improved Cytotoxicity in B16F10 Murine Melanoma Cells

Cited by 3 publications

References 22 publications

Regulation of Cunninghamella spp. biofilm growth by tryptophol and tyrosol

Regulation of Cunninghamella spp. biofilm growth by tryptophol and tyrosol

Sweetening the Deal: Glycosylation and its Clinical Applications

Natural or synthetic? Classification of common preservatives in food and drug industry by artificial intelligence

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