ZFBONE: An ImageJ toolset for semi-automatic analysis of zebrafish bone structures

Tarasco, Marco; Cordelières, Fabrice P.; Cancela, M. Leonor; Laizé, Vincent

doi:10.1016/j.bone.2020.115480

Cited by 18 publications

(11 citation statements)

References 32 publications

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“…Images were analyzed using ImageJ (version 1.52p). For morphometric analysis, images were processed using ZFBONE macro-toolset for Fiji [ 34 ]. The area of the operculum (OpA), the area of the head (HA), the splanchnocranial area (SA), and the areas positive for reporter signals within the HA ( sp7 + , oc + ) and within the SA ( runx2 + ) were determined using an Intuos M drawing tablet (Wacom, Kazo, Japan).…”

Section: Methodsmentioning

confidence: 99%

The osteogenic and mineralogenic potential of the microalgae Skeletonema costatum and Tetraselmis striata CTP4 in fish models

Carletti,

Rosa,

Pes

et al. 2023

Cell. Mol. Life Sci.

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Skeletal disorders are problematic aspects for the aquaculture industry as skeletal deformities, which affect most species of farmed fish, increase production costs and affect fish welfare. Following recent findings that show the presence of osteoactive compounds in marine organisms, we evaluated the osteogenic and mineralogenic potential of commercially available microalgae strains Skeletonema costatum and Tetraselmis striata CTP4 in several fish systems. Ethanolic extracts increased extracellular matrix mineralization in gilthead seabream (Sparus aurata) bone-derived cell cultures and promoted osteoblastic differentiation in zebrafish (Danio rerio) larvae. Long-term dietary exposure to both extracts increased bone mineralization in zebrafish and upregulated the expression of genes involved in bone formation (sp7, col1a1a, oc1, and oc2), bone remodeling (acp5a), and antioxidant defenses (cat, sod1). Extracts also improved the skeletal status of zebrafish juveniles by reducing the incidence of skeletal anomalies. Our results indicate that both strains of microalgae contain osteogenic and mineralogenic compounds, and that ethanolic extracts have the potential for an application in the aquaculture sector as dietary supplements to support fish bone health. Future studies should also identify osteoactive compounds and establish whether they can be used in human health to broaden the therapeutic options for bone erosive disorders such as osteoporosis.

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Section: Methodsmentioning

confidence: 99%

The osteogenic and mineralogenic potential of the microalgae Skeletonema costatum and Tetraselmis striata CTP4 in fish models

Carletti,

Rosa,

Pes

et al. 2023

Cell. Mol. Life Sci.

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“…Fluorescence images were analyzed using ImageJ software version 2.0.0-rc-69/1.52p. For morphometric analysis, the images were processed by using the toolbox available within the “ZFBONE” macro toolset for Fiji ( 35 ). The area of the operculum and the area of the head were digitally measured with the support of an Intous M drawing tablet (Wacom, Ōtone, Japan).…”

Section: Methodsmentioning

confidence: 99%

Antioxidant and Anti-inflammatory Extracts From Sea Cucumbers and Tunicates Induce a Pro-osteogenic Effect in Zebrafish Larvae

et al. 2022

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Bone metabolic disorders such as osteoporosis are characterized by the loss of mineral from the bone tissue leading to its structural weakening and increased susceptibility to fractures. A growing body of evidence suggests that inflammation and oxidative stress play an important role in the pathophysiological processes involved in the rise of these conditions. As the currently available therapeutic strategies are often characterized by toxic effects associated with their long-term use, natural antioxidants and anti-inflammatory compounds such as polyphenols promise to be a valuable alternative for the prevention and treatment of these disorders. In this scope, the marine environment is becoming an important source of bioactive compounds with potential pharmacological applications. Here, we explored the bioactive potential of three species of holothurians (Echinodermata) and four species of tunicates (Chordata) as sources of antioxidant and anti-inflammatory compounds with a particular focus on polyphenolic substances. Hydroethanolic and aqueous extracts were obtained from animals’ biomass and screened for their content of polyphenols and their antioxidant and anti-inflammatory properties. Hydroethanolic fractions of three species of tunicates displayed high polyphenolic content associated with strong antioxidant potential and anti-inflammatory activity. Extracts were thereafter tested for their capacity to promote bone formation and mineralization by applying an assay that uses the developing operculum of zebrafish (Danio rerio) to assess the osteogenic activity of compounds. The same three hydroethanolic fractions from tunicates were characterized by a strong in vivo osteogenic activity, which positively correlated with their anti-inflammatory potential as measured by COX-2 inhibition. This study highlights the therapeutic potential of polyphenol-rich hydroethanolic extracts obtained from three species of tunicates as a substrate for the development of novel drugs for the treatment of bone disorders correlated to oxidative stress and inflammatory processes.

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“…Scales were imaged on binocular microscope using Leica Application Suite (LAS 3.3, Leica). Scales were assessed for size, demineralized area and circularity using the ZF macro (Tarasco et al 2020).…”

Section: Von Kossa Stainingmentioning

confidence: 99%

foxe1mutant zebrafish show indications of a hypothyroid phenotype and increased sensitivity to ethanol for craniofacial malformations

Raterman,

Wagener,

Zethof

et al. 2024

Preprint

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FOXE1 mutations in humans are associated with Bamforth-Lazarus syndrome, characterized by cleft palate and hypothyroidism. Moreover, polymorphisms of FOXE1 are implicated in non- syndromic cleft palate. Much uncertainty still exists about the function of transcription factor FOXE1 in development. To address this, we have previously developed a foxe1 mutant zebrafish demonstrating mineralization defects in larvae. In the present study, we further investigate the thyroid status and skeletal phenotype of adult foxe1 mutants. Compared to wild type controls, mutant fish have increased expression of hypothalamic tshb, and hepatic dio1 and dio2. In plasma we found higher circulating Mg levels, together these findings are indicative of hypothyroidism. We further observed mineralization defects in scales, likely due to enhanced osteoclast activity as measured by increased expression levels of the markers tracp, ctsk and rankl. Gene-environment interactions in the etiology of FOXE1-related craniofacial abnormalities remain elusive, which prompts the need for models to investigate genotype-phenotype associations. We here investigated whether ethanol exposure increases the risk of developing craniofacial malformations in foxe1 mutant larvae that we compared to wild types. We found in ethanol-exposed mutants an increased incidence of developmental malformations and marked changes in gene expression patterns of cartilage markers (sox9a), apoptotic markers (casp3b), retinoic acid metabolism (cyp26c1), and tissue hypoxia markers (hifaa, hifab). Taken together, this study shows that the foxe1 mutant zebrafish recapitulates phenotypes associated with FOXE1 mutations in human patients and a clear foxe1-ethanol interaction.

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ZFBONE: An ImageJ toolset for semi-automatic analysis of zebrafish bone structures

Cited by 18 publications

References 32 publications

The osteogenic and mineralogenic potential of the microalgae Skeletonema costatum and Tetraselmis striata CTP4 in fish models

The osteogenic and mineralogenic potential of the microalgae Skeletonema costatum and Tetraselmis striata CTP4 in fish models

Antioxidant and Anti-inflammatory Extracts From Sea Cucumbers and Tunicates Induce a Pro-osteogenic Effect in Zebrafish Larvae

foxe1mutant zebrafish show indications of a hypothyroid phenotype and increased sensitivity to ethanol for craniofacial malformations

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