Zeta potential of motile spores of the green alga <i>Ulva linza</i> and the influence of electrostatic interactions on spore settlement and adhesion strength

Rosenhahn, Axel; Finlay, John A.; Pettit, Michala E.; Ward, Andrew D.; Wirges, Werner; Gerhard, Reimund; Callow, Maureen E.; Grunze, M.; Callow, James A.

doi:10.1116/1.3110182

Cited by 56 publications

(61 citation statements)

References 40 publications

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“…The Callow group established an assay that allows spores to settle within 45 min to surfaces in order to compare the spore accumulation rate on different surfaces and thus to discriminate their non-fouling potential [62]. A vast number of experiments demonstrated that the settlement kinetics of zoospores of Ulva is affected by a number of physical and chemical surface cues, such as wettability [31,64,67,68], topography [69][70][71][72], and charge [73,74]. Especially self-assembled monolayers (SAM) [75,76] are a versatile class of functional interfaces that are frequently applied in biofouling research, since their mechanical properties are determined by the substrate and thus biological response is solely caused by the surface chemistry.…”

Section: Surface Cues Can Trigger Permanent Adhesion Of Zoospores Of mentioning

confidence: 99%

Surface Sensing and Settlement Strategies of Marine Biofouling Organisms

Rosenhahn

Sendra

2012

Biointerphases

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This review article summarizes some recent insights into the strategies used by marine organisms to select surfaces for colonization. While larger organisms rely on their sensory machinery to select surfaces, smaller microorganisms developed less complex but still effective ways to probe interfaces. Two examples, zoospores of algae and barnacle larvae, are discussed and both appear to have build-in test mechanisms to distinguish surfaces with different physicochemical properties. Some systematic studies on the influence of surface cues on exploration, settlement and adhesion are summarized. The intriguing notion that surface colonization resembles a parallelized surface sensing event is discussed towards its complementarity with conventional surface analytical tools. The strategy to populate only selected surfaces seems advantageous as waves, currents and storms constantly challenge adherent soft and hard fouling organism.

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Section: Surface Cues Can Trigger Permanent Adhesion Of Zoospores Of mentioning

confidence: 99%

Surface Sensing and Settlement Strategies of Marine Biofouling Organisms

Rosenhahn

Sendra

2012

Biointerphases

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“…Even though such attractive surfaces cannot be considered to have antifouling potential, their higher settlement density means they are well-suited to reveal subtle responses to morphology, or as previously shown, to electric fields. [66] The smallest structures (600 nm) have the highest number of settled spores while structures above $1 mm show a reduced settlement density (Fig. 6c).…”

Section: Surface Characterization Of Pem Surfacesmentioning

confidence: 99%

Interaction of Zoospores of the Green Alga Ulva with Bioinspired Micro‐ and Nanostructured Surfaces Prepared by Polyelectrolyte Layer‐by‐Layer Self‐Assembly

Cao

Pettitt

Wode

et al. 2010

Adv Funct Materials

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The interaction of spores of Ulva with bioinspired structured surfaces in the nanometer–micrometer size range is investigated using a series of coatings with systematically varying morphology and chemistry, which allows separation of the contributions of morphology and surface chemistry to settlement (attachment) and adhesion strength. Structured surfaces are prepared by layer‐by‐layer spray‐coating deposition of polyelectrolytes. By changing the pH during application of oppositely charged poly(acrylic acid) and polyethylenimine polyelectrolytes, the surface structures are systematically varied, which allows the influence of morphology on the biological response to be determined. In order to discriminate morphological from chemical effects, surfaces are chemically modified with poly(ethylene glycol) and tridecafluoroctyltriethoxysilane. This chemical modification changes the water contact angles while the influence of the morphology is retained. The lowest level of settlement is observed for structures of the order 2 µm. All surfaces are characterized with respect to their wettability, chemical composition, and morphological properties by contact angle measurement, X‐ray photoelectron spectroscopy, scanning electron microscopy, and atomic force microscopy.

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“…Settlement of zoospores of U. linza is 'selective', i.e., the spores respond to physical and chemical surface cues such as wettability [4][5][6][7], topography [8][9][10][11], and charge [12,13], resulting in clear settlement preferences and therefore variable surface colonization by the alga. The question arises as to how spores exert this selectivity-for example; does it involve different behavioral responses in terms of swimming patterns, trajectories and velocities?…”

Section: Introductionmentioning

confidence: 99%

Settlement Behavior of Zoospores of Ulva linza During Surface Selection Studied by Digital Holographic Microscopy

et al. 2012

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Settlement of the planktonic dispersal stages of marine organisms is the crucial step for the development of marine biofouling. Four-dimensional holographic tracking reveals the mechanism by which algal spores select surfaces suitable for colonization. Quantitative analysis of the three dimensional swimming trajectories of motile spores of a macroalga (Ulva linza) in the vicinity of surfaces functionalized with different chemistries reveals that their search strategy and swimming behavior is correlated to the number of settled spores found in spore settlement bioassays conducted over 45 min. The spore motility and exploration behavior can be classified into different motion patterns, with their relative occurrence changing with the surface chemistry. Based on the detailed motility analysis we derived a model for the surface selection and settlement process of Ulva zoospores.

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Zeta potential of motile spores of the green alga Ulva linza and the influence of electrostatic interactions on spore settlement and adhesion strength

Cited by 56 publications

References 40 publications

Surface Sensing and Settlement Strategies of Marine Biofouling Organisms

Surface Sensing and Settlement Strategies of Marine Biofouling Organisms

Interaction of Zoospores of the Green Alga Ulva with Bioinspired Micro‐ and Nanostructured Surfaces Prepared by Polyelectrolyte Layer‐by‐Layer Self‐Assembly

Settlement Behavior of Zoospores of Ulva linza During Surface Selection Studied by Digital Holographic Microscopy

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