Zerumbone protects human skin keratinocytes against UVA-irradiated damages through Nrf2 induction

Yang, Hsin‐Ling; Lee, Chin-Ling; Korivi, Mallikarjuna; Liao, Jiunn‐Wang; Rajendran, Peramaiyan; Wu, Junyi; Hseu, You‐Cheng

doi:10.1016/j.bcp.2017.12.014

Cited by 55 publications

(58 citation statements)

References 48 publications

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“…EC9706 and HET‐1A cell viability was evaluated using an MTT assay. EC9706 and HET‐1A cells were seeded (10 3 cells/well) onto 96well plates in the presence of RPMI‐1640 medium and then transfected with siRNA vectors at 37°C as described above . Following incubation for 6, 12, 24, 48, and 72 hours at 37°C, 5 mg/mL MTT was added to each well and the cells were incubated for another 4 hours at 37°C.…”

Section: Methodsmentioning

confidence: 99%

RasGRP3, a Ras guanyl releasing protein 3 that contributes to malignant proliferation and aggressiveness in human esophageal squamous cell carcinoma

Zhang

et al. 2018

Clin Exp Pharma Physio

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Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers worldwide; however, clinical and pathological parameters have limited ability in discriminating between clinically significant and indolent ESCC. Since RasGRP3 transcript levels have prognostic value in discriminating ESCC with different clinical aggressiveness, we decided to investigate its putative oncogenic role in ESCC. We found that RasGRP3 was highly expressed in ESCC cells. Suppression of endogenous RasGRP3 expression in esophageal cell lines reduced Ras-GTP formation as well as AKT phosphorylation. RasGRP3 suppression also inhibited cell invasion and migration and reduced proliferation, demonstrating the importance of RasGRP3 for the transformed phenotype of melanoma cells. Suppression of RasGRP3 expression in these cells inhibited downstream RasGRP3 responses and suppressed cell growth and migration, confirming the functional role of RasGRP3 in the altered behaviour of these cells. This suggests that RasGRP3 may function as a Ras activator in the phosphoinositide signalling pathway and may potentially serve as a new therapeutic target.

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Section: Methodsmentioning

confidence: 99%

RasGRP3, a Ras guanyl releasing protein 3 that contributes to malignant proliferation and aggressiveness in human esophageal squamous cell carcinoma

Zhang

et al. 2018

Clin Exp Pharma Physio

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“…As Nrf2 plays a central role in regulating the endogenous antioxidant defense system, activation of Nrf2-ARE pathway is increasingly considered as a promising approach for treatment or prevention of neurodegenerative ailments [5,6]. Thus, extensive interests have been attracted to activate the Nrf2-ARE pathway for therapeutic purposes [7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Reversing ROS‐mediated neurotoxicity by chlorogenic acid involves its direct antioxidant activity and activation of Nrf2‐ARE signaling pathway

Yao

Peng

et al. 2019

BioFactors

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Chlorogenic acid (CA), the ester of caffeic acid and quinic acid, is one of the most abundant polyphenols in coffee, and has multiple pharmacological functions. The present study is designed to explore the protection provided by CA against hydrogen peroxide (H2O2)‐induced oxidative damages in the rat pheochromocytoma cells, and the underlying mechanisms engaged in this process. CA displays robust free radical‐scavenging activity in vitro. More importantly, CA strikingly rescues the cells from the H2O2‐mediated oxidative insults. Mechanistic studies revealed that CA upregulates a panel of phase II cytoprotective species, such as heme oxygenase‐1, NAD(P)H: quinone oxidoreductase 1, glutathione, thioredoxin reductase 1, and thioredoxin 1. This neuroprotection is dependent on the activation of the transcription factor Nuclear factor erythroid 2‐related factor 2 (Nrf2), as knockdown of Nrf2 abolishes such effect. Our results demonstrate that CA provides dual neuroprotection via directly neutralizing free radicals and indirectly inducing expression of Nrf2‐driven cytoprotective enzymes, and suggest a potential therapeutic usage of CA as a neuroprotective agent. Coffee is one of the most popular drinks in the world, and our discovery may also contribute to understanding the beneficial effects of regular coffee consumption. © 2019 BioFactors, 45 (4):616–626, 2019

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“…The signalling pathway of Nrf2 is essential for protection of skin keratinocytes and fibroblasts against damage. In addition, the knockdown of Nrf2 gene exaggerated skin damage . We found that sulforaphane blocked skin cancer‐induced reduction in the gene expression of Nrf2 without affecting the control group.…”

Section: Discussionmentioning

confidence: 73%

Antitumor activity of sulforaphane in mice model of skin cancer via blocking sulfatase‐2

Alyoussef

Taha

2018

Experimental Dermatology

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Although there are many treatment options for skin cancer, the chemotherapeutic agents for skin cancer are linked with many adverse effects as well as the development of multidrug resistance. Sulforaphane is an isothiocyanate, which is found in cruciferous vegetables. Consumption of sulforaphane‐rich diet has been linked to inhibition of UV‐exposed skin carcinogenesis. Therefore, the goal of this study was to determine the ability of sulforaphane to reduce skin cancer in mice through inhibition of sulfatase‐2 enzyme. Epicutaneous application of 7,12‐dimethylbenz (a) anthracene was performed on the shaved dorsal skin of mice followed by croton oil. Sulforaphane (9 μmol/mouse/day) was administered to mice orally. Skin was removed from the dorsal area for assessment of sulfatase‐2, glypican‐3, heparan sulphate proteoglycans (HSPGs), nuclear factor (NF)κB, nuclear factor E2‐related factor 2 (Nrf2), tumor necrosis factor (TNF)‐α, IL‐1β and caspase‐3. In addition, skin sections were stained with haematoxylin/eosin, Mallory and cytokeratin immunostaining. We found that, sulforaphane blocked sulfatase‐2 activity, leading to significant elevation in HSPGs as well as significant reduction in glypican‐3. In addition, sulforaphane significantly activated Nrf2 and reduced both the gene and protein expression of NFκB, TNF‐α, IL‐1β and caspase‐3. In parallel, stained sections obtained from skin cancer mice treated with sulforaphane showed significant reduction in hyperkeratosis, acanthosis and epithelial dysplasia. The collective results indicate that sulforaphane suppresses skin cancer via blocking sulfatase‐2 with subsequent elevation in HSPGs and reduction in glypican‐3. Moreover, sulforaphane attenuated skin cancer‐induced activation of inflammatory and apoptotic pathways.

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Zerumbone protects human skin keratinocytes against UVA-irradiated damages through Nrf2 induction

Cited by 55 publications

References 48 publications

RasGRP3, a Ras guanyl releasing protein 3 that contributes to malignant proliferation and aggressiveness in human esophageal squamous cell carcinoma

RasGRP3, a Ras guanyl releasing protein 3 that contributes to malignant proliferation and aggressiveness in human esophageal squamous cell carcinoma

Reversing ROS‐mediated neurotoxicity by chlorogenic acid involves its direct antioxidant activity and activation of Nrf2‐ARE signaling pathway

Antitumor activity of sulforaphane in mice model of skin cancer via blocking sulfatase‐2

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