Zerumbone from Zingiber zerumbet (L.) smith: a potential prophylactic and therapeutic agent against the cariogenic bacterium Streptococcus mutans

Abstract

BackgroundEssential oil obtained from rhizomes of the Zingiber zerumbet (L.) Smith (popularly known in Brazil as bitter ginger) is mainly constituted by the biomolecule zerumbone, which exhibit untapped antimicrobial potential. The aim of this study was to investigate the antimicrobial activity of the zerumbone from bitter ginger rhizomes against the cariogenic agent Streptococcus mutans.MethodsFirstly, the essential oil from rhizomes of Zingiber zerumbet (L.) Smith extracted by hydrodistillation was submitted to purification and recrystallization process to obtain the zerumbone compound. The purity of zerumbone was determined through high-performance liquid chromatography analysis. Different concentrations of zerumbone were tested against the standard strain S. mutans (ATCC 35668) by using microdilution method. The speed of cidal activity was determined through a time kill-curve assay. The biological cytotoxicity activity of zerumbone was assessed using Vero cell line through MTT assay.ResultsThe zerumbone showed a minimum inhibitory concentration (MIC) of 250 μg/mL and a minimum bactericidal concentration (MBC) of 500 μg/mL against S. mutans. After six hours of bacteria-zerumbone interaction, all concentrations tested starts to kill the bacteria and all bacteria were killed between 48 and 72 h period at the concentration of 500 μg/mL (99,99% of bacteria were killed in comparison with original inoculum). In addition, zerumbone showed no cytotoxicity activity on mammalian continuous cells line.ConclusionsThese results draw attention to the potential of zerumbone as antimicrobial agent against S. mutans infection, indicating its possible use in the phyto-pharmaceutical formulations as new approach to prevent and treat tooth decay disease.Electronic supplementary materialThe online version of this article (10.1186/s12906-018-2360-0) contains supplementary material, which is available to authorized users.

Complete chloroplast genomes of Zingiber montanum and Zingiber zerumbet: Genome structure, comparative and phylogenetic analyses

“…Z. zerumbet, commonly known as "shampoo ginger", is found across southern China (Guangdong, Guangxi, Hainan and Yunnan provinces), most of Southeast Asia, Myanmar, India, and Sri Lanka [1][2][3][4]. Zerumbone from the Z. zerumbet rhizome has been reported to suppress the phagocytic activity of human neutrophils [9], to prevent and treat tooth decay disease [10], to cure osteoarthritis of the knee [11], and to treat various immune-inflammatory related disorders [12].…”

“…Zerumbone from the Z . zerumbet rhizome has been reported to suppress the phagocytic activity of human neutrophils [ 9 ], to prevent and treat tooth decay disease [ 10 ], to cure osteoarthritis of the knee [ 11 ], and to treat various immune-inflammatory related disorders [ 12 ].…”

Protective Effects of Zerumbone on Colonic Tumorigenesis in Enterotoxigenic Bacteroides fragilis (ETBF)-Colonized AOM/DSS BALB/c Mice

“…However, there exist no studies investigating the suppressive effects of natural product on ETBF-mediated tumorigenesis in mice. Previous research has demonstrated health-promoting properties of various dietary natural compounds, including carotenoids, flavonoids, and polyphenols contained in vegetables and fruits [18,19]. Chemoprevention is defined as the administration of dietary natural compounds and/or compounds chemically synthesized that prevents and/or delays the development of cancers.…”

“…Recent studies revealed several biological properties of zerumbone that may be responsible for inhibition of ETBF-induced colon carcinogenesis. Zerumbone has been reported to have anti-bacterial, anti-inflammatory and anti-cancer activities [19][20][21][22][23]. Zerumbone decreases ETBF infection-induced colon inflammation in mice via suppression of NF-κB signaling irrespective of ETBF colonization [9].…”

Atividade antimicrobiana de um nanocompósito de nanocristais baseado em óxido de zinco dopado com nanocristais de prata e óxido de prata

“…Ag mostra uma atividade antibacteriana melhor para as bactérias gram-negativas do que gram-positivas, enquanto ZnO mostra uma melhor atividade antibacteriana para as bactérias gram-positivas.Nesse estudo utilizamos a bactéria Streptococcus mutans, principal agente causador da cárie dentária, a doença infecciosa oral mais prevalente do mundo(PETERSEN, 2014). A S. mutans possui capacidade de aderir às superfícies dentárias, é uma bactéria gram-positiva, acidogênica e possui capacidade de sobreviver em ambientes com baixo pH (MOREIRA DA SILVA et al, 2018). de íons de Zn2 + , relacionada com acumulação de NP ZnO em células bacterianas e formação deROS (KASEMETS et al, 2009).…”