Zeolite‐containing mixture alleviates microbial dysbiosis in dextran sodium sulfate‐induced colitis in mice

Abstract

Inflammatory bowel disease (IBD) is a multifactorial immunomodulatory disorder. In relative nosogenesis, gut microbiota has been the focus of research on IBD. In our previous study, we demonstrated the ameliorating effect of zeolite‐containing mixture (Hydryeast®, HY) on dextran sodium sulfate (DSS)‐induced colitis, through transcriptomics and proteomics. In the present study, we performed further investigation from the perspective of metagenomics using the gut microbiota. C57BL6 mice were provided an AIN‐93G basal diet or a 0.8% HY‐containing diet, and sterilized tap water for 11 days. Thereafter, colitis was induced by providing 1.5% (w/v) DSS‐containing water for 9 days. DNA was extracted from the cecal contents and pooled into libraries in a single Illumina MiSeq run. The resulting sequences were analyzed using Quantitative Insights Into Microbial Ecology (QIIME) software. According to the alterations in the relative abundance of certain bacteria, and the related gene and protein expressions, HY supplementation could improve the gut microbiota composition, ameliorate the degree of inflammation, inhibit the colonic mucosal microbial growth, and, to some extent, promote energy metabolism in the colon compared with the DSS treatment. Thus, we believe that HY may be a candidate to prevent and treat IBD.

Effect of the Additional Natural Compound in Litter on Performance and Health Parameter of Broiler

“…The improvement observed in broiler growth may be attributed to several reasons. For example, it is well-known that zeolites possess cation-exchange properties that limit the growth of pathogenic bacteria, thereby creating a healthy environment (Lyu et al, 2021). The results of the immune organ relative weight in Table 2 showed that the addition of different compounds of broiler litter had a significant effect on the relative weight of the liver (P<0.05) but had no significant effect on the spleen, thymus, and bursa Fabricius (P>0.05).…”

Clinical Parameters in Osteoporosis Patients Supplemented With PMA-Zeolite at the End of 5-Year Double-Blinded Clinical Trial

“…Proven detoxifying properties of the clinoptilolite materials including PMA-zeolite may also improve the overall health and bone status ( 23 , 24 ). At last, local effects in the intestine as the primary site of PMA-zeolite action, i.e., on the microbiota status, as well as corresponding anti-inflammatory and immunomodulatory effects, are likely to contribute to the bone health as well ( 25 , 26 ).…”

Study on relationship between bacterial diversity and quality of Huangjiu (Chinese Rice Wine) fermentation

“…With the advance of high‐throughput sequencing, metagenomic sequencing detecting microbial diversity in the whole environmental system has been increasingly mature. Because of huge data volume, ability to detect all microbes including uncultured ones, metagenomic sequencing has been extensively used in fecal and food microbiota analysis (Chen et al., 2020; Lyu et al., 2021; Mao et al., 2015; Xie et al., 2013). For example, Liu et al (Liu et al., 2018) identified Burkholderia and Bacillus as predominant bacterial genus in Gu Tian Qu (71.62%) and Wu Yi Hong Qu (44.73%), respectively, and detected 213 bacterial genus with low abundance via Illumina‐based metagenomic sequencing.…”