Zellelektrophoretische Charakterisierung der Oberfläche von Candida guilliermondii

Abstract

The electrical properties of the outer layer of the cell wall of a hydrocarbon-grown yeast C . guilliermondii were studied in detail by cell electrophoresis. Some experiments were also made with the yeast 8. cerewisiae. The mobilities of the yeasts were measured as a function of pH a t pH 2-11.5 and constant ionic strength I = 0.02 mol/l. For identification of surface groups the pHmobility curves are used to calculate pK-values. The results indicate the presence of amino and carboxyl groups on the surface of C. guilliermondii. These groups are probably a part of a glycoprotein with an isoelectric point a t pH 3.3 located on the surface. Electrophoresis of droplets of mineral oil coated with adsorbed protein layer confirms these conclusions. The importance of this glycoprotein in hydrocarbon uptake is discussed.