Zebrafishstat3 is expressed in restricted tissues during embryogenesis andstat1 rescues cytokine signaling in aSTAT1-deficient human cell line

Oates, Andrew C.; Wollberg, Patrik; Pratt, Stephen J.; Paw, Barry H.; Johnson, Stephen L.; Ho, Ruoya; Postlethwait, John H.; Zon, Leonard I.; Wilks, Andrew F.

doi:10.1002/(sici)1097-0177(199908)215:4<352::aid-aja7>3.0.co;2-j

Cited by 107 publications

(11 citation statements)

References 111 publications

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“…Previous expression data, along with data presented here has revealed that piscine STAT1 is widely expressed in many tissues [30,31,46-49]. However, data concerning functional activity in lower vertebrates, such as STAT1 phosporylation and cellular localization upon stimulation of cells, is scarce.…”

Section: Discussionmentioning

confidence: 78%

Structural and functional studies of STAT1 from Atlantic salmon (Salmo salar)

Skjesol¹,

Hansen²,

Shi

et al. 2010

BMC Immunol

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BackgroundType I and type II interferons (IFNs) exert their effects mainly through the JAK/STAT pathway, which is presently best described in mammals. STAT1 is involved in signaling pathways induced by both types of IFNs. It has a domain-like structure including an amino-terminus that stabilizes interaction between STAT dimers in a promoter-binding situation, a coiled coil domain facilitating interactions to other proteins, a central DNA-binding domain, a SH2 domain responsible for dimerization of phosphorylated STATs and conserved phosphorylation sites within the carboxy terminus. The latter is also the transcriptional activation domain.ResultsA salmon (Salmo salar) STAT1 homologue, named ssSTAT1a, has been identified and was shown to be ubiquitously expressed in various cells and tissues. The ssSTAT1a had a domain-like structure with functional motifs that are similar to higher vertebrates. Endogenous STAT1 was shown to be phosphorylated at tyrosine residues both in salmon leukocytes and in TO cells treated with recombinant type I and type II IFNs. Also ectopically expressed ssSTAT1 was phosphorylated in salmon cells upon in vitro stimulation by the IFNs, confirming that the cloned gene was recognized by upstream tyrosine kinases. Treatment with IFNs led to nuclear translocation of STAT1 within one hour. The ability of salmon STAT1 to dimerize was also shown.ConclusionsThe structural and functional properties of salmon STAT1 resemble the properties of mammalian STAT1.

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Section: Discussionmentioning

confidence: 78%

Structural and functional studies of STAT1 from Atlantic salmon (Salmo salar)

Skjesol¹,

Hansen²,

Shi

et al. 2010

BMC Immunol

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“…Although the ERV-9 LTRs are found only in primates (1,4,6), we assumed that the LTR enhancer would function in Tg zebrafish, because transcription factors that may bind to the GATA, CCAAT, and CACCC motifs and activate the LTR enhancer are expressed in similar temporal fashion in zebrafish as in man and mouse (18)(19)(20). Breeding of the Tg zebrafish lines showed that in the Tg embryos, GFP expression was maternally inherited.…”

mentioning

confidence: 99%

The LTR enhancer of ERV-9 human endogenous retrovirus is active in oocytes and progenitor cells in transgenic zebrafish and humans

Yang

Wang

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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The solitary LTRs of ERV-9 human endogenous retrovirus are middle repetitive DNAs associated with 3,000 -4,000 human gene loci including the ␤-globin gene locus where the ERV-9 LTR is juxtaposed to the locus control region (␤-LCR) far upstream of the globin genes. The ERV-9 LTRs are conserved during primate evolution, but their function in the primate genomes is unknown. Here, we show that in transgenic zebrafish harboring the ␤-globin ERV-9 LTR coupled to the GFP gene, the LTR enhancer was active and initiated synthesis of GFP mRNA in oocytes but not in spermatozoa, and GFP expression in the embryos was maternally inherited. The LTR enhancer was active also in stem͞ progenitor cell regions of adult tissues of transgenic zebrafish. In human tissues, ERV-9 LTR enhancer was active also in oocytes and stem͞progenitor cells but not in spermatozoa and a number of differentiated, adult somatic cells. Transcriptional analyses of the human ␤-globin gene locus showed that the ␤-globin ERV-9 LTR enhancer initiated RNA synthesis from the LTR in the direction of the downstream ␤ locus control region and globin genes in ovary and erythroid progenitor cells. The findings suggest that, during oogenesis, ERV-9 LTR enhancers in the human genome could activate the cis-linked gene loci to synthesize maternal mRNAs required for early embryogenesis. Alternatively, the ERV-9 LTR enhancers, in initiating RNA syntheses into the downstream genomic DNAs, could transcriptionally potentiate and preset chromatin structure of the cis-linked gene loci in oocytes and adult stem͞progenitor cells.

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“…LIF activation through the JAK1/STAT3 pathway has been shown to induce SOCS4 expression during ovarian follicular development [ 9 ], consistent with the strong levels of maternally derived socs4a transcripts observed. Moreover, the expression of zebrafish stat3 overlaps strongly with that of socs4a during embryogenesis, including in a variety of sensory neural structures, such as the ALLG, PLLG, TG and SAG [ 40 , 41 ]. Interestingly, zebrafish stat3 has been implicated in convergence/extension [ 40 ], with stat3 mutants displaying reduced A-P extension [ 42 ], as observed in socs4a morphants.…”

Section: Discussionmentioning

confidence: 99%

Functional Analysis of Zebrafish socs4a: Impacts on the Notochord and Sensory Function

et al. 2022

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The suppressor of cytokine signaling (SOCS) proteins play important roles in cytokine and growth factor signaling, where they act principally as negative feedback regulators, particularly of the downstream signal transducer and activator of transcription (STAT) transcription factors. This critical mode of regulation impacts on both development and homeostasis. However, understanding of the function of SOCS4 remains limited. To address this, we investigated one of the zebrafish SOCS4 paralogues, socs4a, analyzing its expression and the consequences of its ablation. The socs4a gene had a dynamic expression profile during zebrafish embryogenesis, with initial ubiquitous expression becoming restricted to sensory ganglion within the developing nervous system. The knockdown of zebrafish socs4a revealed novel roles in notochord development, as well as the formation of a functional sensory system.

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Zebrafishstat3 is expressed in restricted tissues during embryogenesis andstat1 rescues cytokine signaling in aSTAT1-deficient human cell line

Cited by 107 publications

References 111 publications

Structural and functional studies of STAT1 from Atlantic salmon (Salmo salar)

Structural and functional studies of STAT1 from Atlantic salmon (Salmo salar)

The LTR enhancer of ERV-9 human endogenous retrovirus is active in oocytes and progenitor cells in transgenic zebrafish and humans

Functional Analysis of Zebrafish socs4a: Impacts on the Notochord and Sensory Function

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