Zebrafish stm is involved in the development of otoliths and of the fertilization envelope

Pachoensuk, Theeranukul; Fukuyo, Taketo; Rezanujjaman, Md.; Klangnurak, Wanlada; Yamamoto, Chihiro; Maeno, Akiteru; Rahaman, Md. Mostafizur; Ali, Md. Hasan; Tokumoto, Toshinobu

doi:10.1530/raf-20-0040

Cited by 6 publications

(3 citation statements)

References 22 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By comparison, the stm -deficient embryos showed the phenotype of otolith aggregation but not a single otolith compared with BPA-induced otolith malformations. This also agrees well with previous studies using both knockout and knockdown approaches. , These results suggest that the dysfunction of otop1 was more likely responsible for the BPA-induced otolith malformation than stm . A previous study has shown that Otop1 affects the localization of Stm.…”

Section: Discussionmentioning

confidence: 94%

See 1 more Smart Citation

Genetic and Epigenetic Evidence for Nonestrogenic Disruption of Otolith Development by Bisphenol A in Zebrafish

Yuan,

Zeng,

et al. 2023

Environ. Sci. Technol.

View full text Add to dashboard Cite

Bisphenol A (BPA) is a well-known endocrine-disrupting chemical (EDC) that has estrogenic activities. In addition to disrupting reproductive development and function via estrogenic signaling pathways, BPA can also interfere with nonreproductive functions through nonestrogenic pathways; however, the mechanisms underlying such nonestrogenic activities are not well understood. In this study, we demonstrated that BPA could disrupt otolith formation during the early development of zebrafish with long-lasting ethological effects. Using multiple mutants of estrogen receptors, we provided strong genetic evidence that the BPA-induced otolith malformation was independent of estrogen signaling. Transcriptome analysis revealed that two genes related to otolith development, otopetrin 1 (otop1) and starmaker (stm), decreased their expression significantly after BPA exposure. Knockout of both otop1 and stm genes could phenocopy the BPA-induced otolith malformation, while microinjection of their mRNAs could rescue the BPA-induced abnormalities of otolith formation. Further experiments showed that BPA inhibited the expression of otop1 and stm by activating the MEK/ERK-EZH2-H3K27me3 signaling pathway. Taken together, our study provided comprehensive genetic and molecular evidence that BPA induced the otolith malformation through nonestrogenic pathway during zebrafish early development and its activities involved epigenetic control of key genes (e.g., otop1 and stm) participating in otolith formation.

show abstract

Section: Discussionmentioning

confidence: 94%

“…This also agrees well with previous studies using both knockout and knockdown approaches. 42,44 These results suggest that the dysfunction of otop1 was more likely responsible for the BPA-induced otolith malformation than stm. A previous study has shown that Otop1 affects the localization of Stm.…”

Section: ■ Discussionmentioning

confidence: 99%

Genetic and Epigenetic Evidence for Nonestrogenic Disruption of Otolith Development by Bisphenol A in Zebrafish

Yuan,

Zeng,

et al. 2023

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…Interestingly, vtg1 mutants had an increased number of eggs, although the larvae died due to multiple developmental defects during late developmental stages [ 334 ]. Defective eggs with disrupted pineal structures in the shell are formed in mutants of the stm gene [ 335 ].…”

Section: Genome Editing In Salmonidae and Cyprinidae Aquaculture Fish...mentioning

confidence: 99%

In Search of a Target Gene for a Desirable Phenotype in Aquaculture: Genome Editing of Cyprinidae and Salmonidae Species

Orlova,

Ruzina,

Emelianova

et al. 2024

Genes

View full text Add to dashboard Cite

Aquaculture supplies the world food market with a significant amount of valuable protein. Highly productive aquaculture fishes can be derived by utilizing genome-editing methods, and the main problem is to choose a target gene to obtain the desirable phenotype. This paper presents a review of the studies of genome editing for genes controlling body development, growth, pigmentation and sex determination in five key aquaculture Salmonidae and Cyprinidae species, such as rainbow trout (Onchorhynchus mykiss), Atlantic salmon (Salmo salar), common carp (Cyprinus carpio), goldfish (Carassius auratus), Gibel carp (Carassius gibelio) and the model fish zebrafish (Danio rerio). Among the genes studied, the most applicable for aquaculture are mstnba, pomc, and acvr2, the knockout of which leads to enhanced muscle growth; runx2b, mutants of which do not form bones in myoseptae; lepr, whose lack of function makes fish fast-growing; fads2, Δ6abc/5Mt, and Δ6bcMt, affecting the composition of fatty acids in fish meat; dnd mettl3, and wnt4a, mutants of which are sterile; and disease-susceptibility genes prmt7, gab3, gcJAM-A, and cxcr3.2. Schemes for obtaining common carp populations consisting of only large females are promising for use in aquaculture. The immobilized and uncolored zebrafish line is of interest for laboratory use.

show abstract

Applications of genome editing in fish development and disease

Zhang,

2024

Encyclopedia of Fish Physiology

View full text Add to dashboard Cite

Zebrafish stm is involved in the development of otoliths and of the fertilization envelope

Cited by 6 publications

References 22 publications

Genetic and Epigenetic Evidence for Nonestrogenic Disruption of Otolith Development by Bisphenol A in Zebrafish

Genetic and Epigenetic Evidence for Nonestrogenic Disruption of Otolith Development by Bisphenol A in Zebrafish

In Search of a Target Gene for a Desirable Phenotype in Aquaculture: Genome Editing of Cyprinidae and Salmonidae Species

Applications of genome editing in fish development and disease

Contact Info

Product

Resources

About