Zebrafish Melanophores: A Model for Teaching Second Messenger Systems

Abstract

A strong literature base supports the notion that active learning improves retention in the science classroom. To that end, a course was designed to allow students to develop their own experiments around a central biological question. The model system used in this particular course is control of melanosome dispersal via second messenger systems in zebrafish (Danio rerio) scales. Students start by applying agonists and antagonists to the cAMP and Ca(2+) second messenger systems, and then can progress to more refined questions with the model system. This project is advantageous because it could be easily adapted to fit the needs of many different courses and ability levels; it is relatively easy to perform; it is enjoyable to teach; and students can be largely given a free reign to decide upon the design of their experiments.

Development of an undergraduate cell biology laboratory to assess pigmentation and cell size in a zebrafish model of uveal melanoma

“…The zebrafish (Danio rerio) is a popular model organism not only in scientific research laboratories [1][2][3][4] , but also in undergraduate education settings 5,6 and for K-12 outreach [7][8][9][10][11] . This small freshwater fish is used as a model across many subfields of biology and biomedical science, including cell biology, developmental biology, genetics, neuroscience, immunology, and cancer biology 12 .…”