Zebrafish mast cells possess an FcɛRI-like receptor and participate in innate and adaptive immune responses

Da’as, Sahar; Teh, Evelyn M.; Dobson, J. Tristan; Nasrallah, Gheyath K.; McBride, Eileen; Wang, Hao; Neuberg, Donna; Marshall, Jean S.; Lin, Tong‐Jun; Berman, Jason N.

doi:10.1016/j.dci.2010.09.001

Cited by 55 publications

(47 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4,7,8 We first described a mast cell counterpart in the zebrafish 9 and subsequently showed conserved roles of these cells in adaptive and innate responses to inflammatory stimuli. 10 Zebrafish carboxypeptidase a5 (cpa5) was identified as a zebrafish mast cell-specific marker, and gata2 and pu.1 were found to be the key transcription factors required for early mast cell lineage commitment in keeping with studies in mammalian systems. 9,11,12 The Notch signaling pathway is a critical regulator of cell fate determination conserved through evolution.…”

Section: Introductionmentioning

confidence: 84%

“…43 The zebrafish model affords the opportunity for elucidating the transcriptional regulation of many vertebrate hematopoietic lineages in vivo, including mast cells. 9,10 We previously identified cpa5 as a unique marker of zebrafish mast cells, and we characterized both pu.1 and gata2 as key transcription factors for primitive zebrafish mast cell development. 9 Our present study confirms the requirement for gata2 in early mast cell development with new insight into its upstream regulation being directed through Notch signaling.…”

Section: Notch In Zebrafish Mast Cells 3589mentioning

confidence: 99%

See 1 more Smart Citation

The zebrafish reveals dependence of the mast cell lineage on Notch signaling in vivo

Da’as

Coombs

Balci

et al. 2012

Blood

Self Cite

View full text Add to dashboard Cite

We used the opportunities afforded by the zebrafish to determine upstream pathways regulating mast cell development in vivo and identify their cellular origin. Colocalization studies demonstrated zebrafish notch receptor expression in cells expressing carboxypeptidase A5 (cpa5), a zebrafish mast cell-specific marker. Inhibition of the Notch pathway resulted in decreased cpa5 expression in mindbomb mutants and wild-type embryos treated with the ␥-secretase inhibitor, Compound E. A series of morpholino knockdown studies specifically identified notch1b and gata2 as the critical factors regulating mast cell fate. Moreover, hsp70::GAL4;UAS::nicd1a transgenic embryos overexpressing an activated form of notch1, nicd1a, displayed increased cpa5, gata2, and pu.1 expression. This increase in cpa5 expression could be reversed and reduced below baseline levels in a dose-dependent manner using Compound E. Finally, evidence that cpa5 expression colocalizes with lmo2 in the absence of IntroductionMast cells are best known for their role in acute and chronic allergic reactions; however, they also function as a crucial component in both innate and acquired immune responses 1 as well as solid tumor and leukemia progression. 2,3 Mast cells delineate from hematopoietic stem cells (HSCs) in the bone marrow but, unlike other blood cells, enter circulation as progenitors. They only complete maturation in resident tissues, which greatly hinders accurate lineage tracing studies in traditional mammalian models. 1 We have been using the zebrafish model to study mast cell development and, specifically, the transcriptional regulation of mast cell lineage commitment. The zebrafish is a highly efficient model system for studying blood cell development. 4-6 All of the major hematopoietic cellular lineages studied to date have zebrafish counterparts, and the fundamental genetic mechanisms that control hematopoiesis are well conserved. 4,7,8 We first described a mast cell counterpart in the zebrafish 9 and subsequently showed conserved roles of these cells in adaptive and innate responses to inflammatory stimuli. 10 Zebrafish carboxypeptidase a5 (cpa5) was identified as a zebrafish mast cell-specific marker, and gata2 and pu.1 were found to be the key transcription factors required for early mast cell lineage commitment in keeping with studies in mammalian systems. 9,11,12 The Notch signaling pathway is a critical regulator of cell fate determination conserved through evolution. Aberrant Notch signaling is associated with a wide range of human disorders from developmental syndromes to cancer. 13 Notch signaling is involved in the fate determination of a variety of cell types, including hematopoietic cells where it participates in differentiation, proliferation, and apoptosis. 14 In mammals, the Notch pathway consists of 4 Notch genes (Notch1-4), which encode transmembrane receptor proteins. These receptors are activated by 5 ligands encoded by the Delta and Serrate/Jagged gene families: Delta-like1, (Dll1), Dll3, Dll4, Jagged 1 (Jag1), and Jag2...

show abstract

Section: Introductionmentioning

confidence: 84%

Section: Notch In Zebrafish Mast Cells 3589mentioning

confidence: 99%

The zebrafish reveals dependence of the mast cell lineage on Notch signaling in vivo

Da’as

Coombs

Balci

et al. 2012

Blood

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, the helix length in Dare-PTX is insufficient for the simultaneous recognition of the two FcR domains, which is called diagonal binding (Lu et al, 2008). In addition, FccR and FcaR have not been observed in zebrafish (Lu et al, 2012(Lu et al, , 2011, and only certain FceRc chain-like genes have been determined, primarily functioning in intracellular signal transduction (Da'as et al, 2011). Therefore, it is difficult to determine the FcR binding capacity for trimeric Dare-PTX.…”

Section: Discussionmentioning

confidence: 99%

Crystal structures for short-chain pentraxin from zebrafish demonstrate a cyclic trimer with new recognition and effector faces

Chen

Yuan

et al. 2015

Journal of Structural Biology

View full text Add to dashboard Cite

“…Studying the role of histamine in mammalian arousal is complicated because, in addition to its neurologic functions, it also plays a central role in humoral and cellular immunity (Haas et al, 2008; Jutel et al, 2009). The zebrafish has recently emerged as a useful model organism to study sleep (Chiu and Prober, 2013) and may be particularly appropriate to explore the role of histamine in arousal, because histamine production is restricted to the TMN (Da'as et al, 2011) and the zebrafish genome appears to lack an ortholog of the peripherally expressed Hrh4 (Peitsaro et al, 2007). Furthermore, with only 10-12 histaminergic neurons, zebrafish larvae provide a simpler model to explore the role of these neurons in sleep (Sundvik et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Genetic Analysis of Histamine Signaling in Larval Zebrafish Sleep

Chen

Singh

Oikonomou

et al. 2017

eNeuro

View full text Add to dashboard Cite

Pharmacological studies in mammals and zebrafish suggest that histamine plays an important role in promoting arousal. However, genetic studies using rodents with disrupted histamine synthesis or signaling have revealed only subtle or no sleep/wake phenotypes. Studies of histamine function in mammalian arousal are complicated by its production in cells of the immune system and its roles in humoral and cellular immunity, which can have profound effects on sleep/wake states. To avoid this potential confound, we used genetics to explore the role of histamine in regulating sleep in zebrafish, a diurnal vertebrate in which histamine production is restricted to neurons in the brain. Similar to rodent genetic studies, we found that zebrafish that lack histamine due to mutation of histidine decarboxylase (hdc) exhibit largely normal sleep/wake behaviors. Zebrafish containing predicted null mutations in several histamine receptors also lack robust sleep/wake phenotypes, although we are unable to verify that these mutants are completely nonfunctional. Consistent with some rodent studies, we found that arousal induced by overexpression of the neuropeptide hypocretin (Hcrt) or by stimulation of hcrt-expressing neurons is not blocked in hdc or hrh1 mutants. We also found that the number of hcrt-expressing or histaminergic neurons is unaffected in animals that lack histamine or Hcrt signaling, respectively. Thus, while acute pharmacological manipulation of histamine signaling has been shown to have profound effects on zebrafish and mammalian sleep, our results suggest that chronic loss of histamine signaling due to genetic mutations has only subtle effects on sleep in zebrafish, similar to rodents.

show abstract

Zebrafish mast cells possess an FcɛRI-like receptor and participate in innate and adaptive immune responses

Cited by 55 publications

References 47 publications

The zebrafish reveals dependence of the mast cell lineage on Notch signaling in vivo

The zebrafish reveals dependence of the mast cell lineage on Notch signaling in vivo

Crystal structures for short-chain pentraxin from zebrafish demonstrate a cyclic trimer with new recognition and effector faces

Genetic Analysis of Histamine Signaling in Larval Zebrafish Sleep

Contact Info

Product

Resources

About