Zebrafish inner ear sensory surfaces are similar to those in goldfish

Platt, Christopher

doi:10.1016/0378-5955(93)90208-i

Cited by 64 publications

(65 citation statements)

References 25 publications

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“…This indicated that a cell proliferation defect occurred prior to formation of the double-layered epithelium leading to a reduced number of precursor cells and thereby a diminished number of hair cells. Confirming this, we found that, as previously described (Platt, 1993;Sobkowicz et al, 1995), each kinocilium in the cdc14b morphant larvae was always associated with an actin-rich structure called stereocilia at the surface of the hair cells (Fig. 5K).…”

Section: Research Articlesupporting

confidence: 87%

The Cdc14B phosphatase contributes to ciliogenesis in zebrafish

2011

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SUMMARYProgression through the cell cycle relies on oscillation of cyclin-dependent kinase (Cdk) activity. One mechanism for downregulating Cdk signaling is to activate opposing phosphatases. The Cdc14 family of phosphatases counteracts Cdk1 phosphorylation in diverse organisms to allow proper exit from mitosis and cytokinesis. However, the role of the vertebrate CDC14 phosphatases, CDC14A and CDC14B, in re-setting the cell for interphase remains unclear. To understand Cdc14 function in vertebrates, we cloned the zebrafish cdc14b gene and used antisense morpholino oligonucleotides and an insertional mutation to inhibit its function during early development. Loss of Cdc14B function led to an array of phenotypes, including hydrocephaly, curved body, kidney cysts and left-right asymmetry defects, reminiscent of zebrafish mutants with defective cilia. Indeed, we report that motile and primary cilia were shorter in cdc14b-deficient embryos. We also demonstrate that Cdc14B function in ciliogenesis requires its phosphatase activity and can be dissociated from its function in cell cycle control. Finally, we propose that Cdc14B plays a role in the regulation of cilia length in a pathway independent of fibroblast growth factor (FGF). This first study of a loss of function of a Cdc14 family member in a vertebrate organism reveals a new role for Cdc14B in ciliogenesis and consequently in a number of developmental processes.

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Section: Research Articlesupporting

confidence: 87%

The Cdc14B phosphatase contributes to ciliogenesis in zebrafish

2011

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“…Regions of the developing macula communis appear to correspond to the utricular and saccular maculae of gnathostomes Comparison of our stage 29 embryonic data with those of Lowenstein et al (Lowenstein et al, 1968) (adult L. fluviatilis), Haddon et al (Haddon et al, 1999) (5-day-old zebrafish) and Platt (Platt, 1993) (adult zebrafish), suggests that the anterior and posterior regions of the macula communis correspond to the utricular and saccular maculae of zebrafish, respectively. It is likely that this can be extrapolated to the utricular and saccular maculae of gnathostomes in general, as there are extensive similarities between polarity patterns in the various groups, although in most cases these have only been studied in the adult ear (Baird, 1974;Popper, 1978;Popper and Northcutt, 1983;Mathiesen and Popper, 1987;Platt, 1993;Denman-Johnson and Forge, 1999;Platt et al, 2004).…”

Section: Discussionmentioning

confidence: 84%

The developing lamprey ear closely resembles the zebrafish otic vesicle:otx1expression can account for all major patterning differences

Hammond

Whitfield

2006

Development

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The inner ear of adult agnathan vertebrates is relatively symmetric about the anteroposterior axis, with only two semicircular canals and a single sensory macula. This contrasts with the highly asymmetric gnathostome arrangement of three canals and several separate maculae. Symmetric ears can be obtained experimentally in gnathostomes in several ways, including by manipulation of zebrafish Hedgehog signalling, and it has been suggested that these phenotypes might represent an atavistic condition. We have found, however, that the symmetry of the adult lamprey inner ear is not reflected in its early development; the lamprey otic vesicle is highly asymmetric about the anteroposterior axis, both morphologically and molecularly, and bears a striking resemblance to the zebrafish otic vesicle. The single sensory macula originates as two foci of hair cells, and later shows regions of homology to the zebrafish utricular and saccular maculae. It is likely, therefore, that the last common ancestor of lampreys and gnathostomes already had well-defined otic anteroposterior asymmetries. Both lamprey and zebrafish otic vesicles express a target of Hedgehog signalling, patched, indicating that both are responsive to Hedgehog signalling. One significant distinction between agnathans and gnathostomes, however, is the acquisition of otic Otx1 expression in the gnathostome lineage. We show that Otx1 knockdown in zebrafish, as in Otx1 -/-mice, gives rise to lamprey-like inner ears. The role of Otx1 in the gnathostome ear is therefore highly conserved; otic Otx1 expression is likely to account not only for the gain of a third semicircular canal and crista in gnathostomes, but also for the separation of the zones of the single macula into distinct regions.

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“…Based on the size of the zebrafish used by Higgs et al (2003), their ages likely ranged from 1 month old to adult. At 1-month post fertilization, zebrafish have an inner ear whose gross morphology resembles the inner ear of adult zebrafish, including the three otolithic organs and three semicircular canals with associated ampullae (Platt 1993;Bang et al 2001). Second, different independent variables and recording methods were used in the present study compared to the study by Higgs et al (2003).…”

Section: Development Of Structure and Function Of The Inner Earmentioning

confidence: 99%

“…The lagena does not appear until 11 days post fertilization (dpf). The inner ear of adult zebrafish has three pairs of otolith organs, each of which is composed of a calcareous otolith and a sensory epithelium covered with hair cells (Platt 1993;Bang et al 2001;Bever and Fekete 2002). The utricle is located in the pars superior whereas the saccule and the lagena are in the pars inferior.…”

Section: Introductionmentioning

confidence: 99%

Early Development of Hearing in Zebrafish

DeSmidt

2013

JARO

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The zebrafish (Danio rerio) has become a valuable vertebrate model for human hearing and balance disorders because it combines powerful genetics, excellent embryology, and exceptional in vivo visualization in one organism. In this study, we investigated auditory function of zebrafish at early developmental stages using the microphonic potential method. This is the first study to report ontogeny of response of hair cells in any fish during the first week post fertilization. The right ear of each zebrafish embedded in agarose was linearly stimulated with a glass probe that was driven by a calibrated piezoelectric actuator. Using beveled micropipettes filled with standard fish saline, extracellular microphonic potentials were recorded from hair cells in the inner ear of zebrafish embryos or larvae in response to 20, 50, 100, and 200-Hz stimulation. Saccular hair cells expressing green fluorescent protein of the transgenic zebrafish from 2 to 7 days post fertilization (dpf) were visualized and quantified using confocal microscopy. The otic vesicles' areas, otoliths' areas, and saccular hair cell count and density increased linearly with age and standard body length. Microphonic responses increased monotonically with stimulus intensity, stimulus frequency, and age of zebrafish. Microphonic threshold at 200 Hz gradually decreased with zebrafish age. The increases in microphonic response and sensitivity correlate with the increases in number and density of hair cells in the saccule. These results enhance our knowledge of early development of auditory function in zebrafish and provide the control data that can be used to evaluate hearing of young zebrafish morphants or mutants.

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Zebrafish inner ear sensory surfaces are similar to those in goldfish

Cited by 64 publications

References 25 publications

The Cdc14B phosphatase contributes to ciliogenesis in zebrafish

The Cdc14B phosphatase contributes to ciliogenesis in zebrafish

The developing lamprey ear closely resembles the zebrafish otic vesicle:otx1expression can account for all major patterning differences

Early Development of Hearing in Zebrafish

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