Zebrafish <i>acvr2a</i> and <i>acvr2b</i> exhibit distinct roles in craniofacial development

Albertson, R. Craig; Payne-Ferreira, Tracie L.; Postlethwait, John H.; Yelick, Pamela C.

doi:10.1002/dvdy.20480

Cited by 31 publications

(18 citation statements)

References 69 publications

(74 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5A-C) and expression possibly overlapped dlx2 -expressing cells consistent with cranial neural crest cells migrating posterior to the pharyngeal mandibular arch and along the hyoid arch and brachial arch (Fig. 5D,E; Albertson et al, 2005). Limited expression was observed in forebrain and midbrain structures.…”

Section: Resultsmentioning

confidence: 75%

Cloning and spatiotemporal expression of zebrafish neuronal nicotinic acetylcholine receptor alpha 6 and alpha 4 subunit RNAs

Ackerman

Nakkula

Zirger

et al. 2009

Developmental Dynamics

View full text Add to dashboard Cite

Acetylcholine plays an important role in regulation of nervous system development and function. We are developing zebrafish (Danio rerio) as a model system to study the role of specific neuronal nicotinic acetylcholine receptor (nAChR) subtypes in development and the effects of nicotine on the developing vertebrate nervous system. We previously characterized the expression of several zebrafish nAChR subunits. To further develop the zebrafish model, here we report a study on the molecular characterization of two additional nAChR subunit genes, designated chrna6 and chrna4. Both zebrafish nAChRs have a high degree of sequence identity to nAChRs expressed in a variety of mammalian species. Reverse transcription polymerase chain reaction was used to show that both nAChR subunit RNAs were expressed early in zebrafish development, with the chrna4 transcript present at 3 hours postfertilization (hpf) and the chrna6 RNA present at 10 hpf. In situ hybridization was used to localize chrna6 and chrna4 RNA expression in 24, 48, 72, and 96 hpf zebrafish. The chrna6 and chrna4 RNAs were each expressed in a unique pattern, which changed during development. At various ages, chrna6 was expressed in Rohon-Beard sensory neurons, trigeminal ganglion, retina, and the pineal gland. Most notably, chrna6 was expressed in catecholaminergic neurons in the midbrain, but was also present in noncatecholaminergic cells in both midbrain and hindbrain. The expression of chrna6 RNA in catecholaminergic cells supports the use of zebrafish as a valid model system to better understand the molecular basis of cholinergic regulation of dopaminergic signaling and the role of α6-containing nAChRs in Parkinson’s disease. The most notable chrna4 expression was in neural crest cells at 24 hpf and reticulospinal neurons in hindbrain at 48 hpf. chrna4 RNA exhibited a widespread and robust expression pattern in the midbrain in 72 hpf and 96 hpf zebrafish.

show abstract

Section: Resultsmentioning

confidence: 75%

Cloning and spatiotemporal expression of zebrafish neuronal nicotinic acetylcholine receptor alpha 6 and alpha 4 subunit RNAs

Ackerman

Nakkula

Zirger

et al. 2009

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

“…Interestingly, among those predicted targets, two genes, Acvr2a and Ndst1, which play important roles in skeletogenesis 82,83 , are predicted to be targets for both miR199 and miR214 in all three vertebrate species studied, suggesting a cooperative action of this miR cluster in the regulation of expression of those two protein coding genes. Furthermore, many other transcripts are shown to be targeted by both miRs but not conserved across all three species (Fig.…”

Section: Discussionmentioning

confidence: 99%

Evolution of themiR199-214cluster and vertebrate skeletal development

2014

View full text Add to dashboard Cite

The miR199 and miR214 genes occupy an intronic cluster located on the opposite strand of the Dynamin3 gene. These miRNAs play major roles in a broad variety of developmental processes and diseases, including skeletal development and several types of cancer. In the work reported here, we first deciphered the origin of the miR199 and miR214 families by following evolution of miR paralogs and their host Dynamin paralogs. We then examined the expression patterns of miR199 and miR214 in developing zebrafish embryos and demonstrated their regulation through a common primary transcript. Results suggest an evolutionarily conserved regulation across vertebrate lineages. Our expression study showed predominant expression patterns for both miR in tissues surrounding developing craniofacial skeletal elements consistent with expression data in mouse and human, thus indicating a conserved role of miR199 and miR214 in vertebrate skeletogenesis.

show abstract

“…The effects of the xGalntl-1 morpholinos on the formation of the spinal cord were rather mild, but the lack of anterior neural crest and the reduction of neural tissue suggest an important role of xGalntl-1 in the regulation of proliferation and differentiation of these tissues. Interestingly, the morpholino knock-down of ActR-IIA and ActR-IIB in zebrafish revealed a requirement for both receptor subtypes in the formation of cranial neural crest and neural tissue (Albertson et al, 2005). In addition, overexpression of xActR-IIB in the early Xenopus embryo inhibited msx1 expression at neurula stage (Fig.…”

Section: Negative Regulation Of Tgf-β Signalling By Galntl-1mentioning

confidence: 89%

Regulation of TGF-β signalling by N-acetylgalactosaminyltransferase-like 1

et al. 2008

View full text Add to dashboard Cite

The TGF-β superfamily of secreted signalling molecules plays a pivotal role in the regulation of early embryogenesis, organogenesis and adult tissue homeostasis. Here we report the identification of Xenopus N-acetylgalactosaminyltransferase-like 1 (xGalntl-1) as a novel important regulator of TGF-β signalling. N-acetylgalactosaminyltransferases mediate the first step of mucin-type glycosylation, adding N-acetylgalactose to serine or threonine side chains. xGalntl-1 is expressed in the anterior mesoderm and neural crest territory at neurula stage, and in the anterior neural crest, notochord and the mediolateral spinal cord at tailbud stage. Inhibition of endogenous xGalntl-1 protein synthesis, using specific morpholino oligomers, interfered with the formation of anterior neural crest, anterior notochord and the spinal cord. Xenopus and mammalian Galntl-1 inhibited Activin as well as BMP signalling in the early Xenopus embryo and in human HEK 293T cells. Gain-and loss-of-function experiments showed that xGalntl-1 interferes with the activity of the common TGF-β type II receptor ActR-IIB in vivo. In addition, our biochemical data demonstrated that xGalntl-1 specifically interferes with the binding of ActR-IIB to Activin-and BMP-specific type I receptors. This inhibitory activity of xGalntl-1 was dependent on mucin-type glycosylation, as it was sensitive to the chemical inhibitor benzyl-GalNAc. These studies reveal an important role of a N-acetylgalactosaminyltransferase in the regulation of TGF-β signalling. This novel regulatory mechanism is evolutionarily conserved and, thus, might provide a new paradigm for the regulation of TGF-β signalling in vertebrates.

show abstract

Zebrafish acvr2a and acvr2b exhibit distinct roles in craniofacial development

Cited by 31 publications

References 69 publications

Cloning and spatiotemporal expression of zebrafish neuronal nicotinic acetylcholine receptor alpha 6 and alpha 4 subunit RNAs

Cloning and spatiotemporal expression of zebrafish neuronal nicotinic acetylcholine receptor alpha 6 and alpha 4 subunit RNAs

Evolution of themiR199-214cluster and vertebrate skeletal development

Regulation of TGF-β signalling by N-acetylgalactosaminyltransferase-like 1

Contact Info

Product

Resources

About