Zebrafish Hsp40 and Hsc70 genes are both induced during caudal fin regeneration

Tawk, Marcel; Joulie, Catherine; Vriz, Sophie

doi:10.1016/s0925-4773(00)00478-0

Cited by 21 publications

(13 citation statements)

References 16 publications

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“…While HSC70 acts as an important chaperone in non-stressed cells, its role upon exposure to stress is unknown. Our results are in line with previous studies in which HSC70 induction was observed upon exposure of Zebrafish embryos to heat shock [16] and during caudal fin regeneration [17]. Together with the HSP70 family of proteins, HSP90 regulates steroid–hormone response, protein translocation and degradation [18], all crucial systems in chronic stress.…”

Section: Discussionsupporting

confidence: 92%

Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress

et al. 2014

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Heat shock proteins (HSPs), originally identified as heat-inducible gene products, are a family of highly conserved proteins that respond to a wide variety of stress including oxidative stress. Although both acute and chronic oxidative stress have been well demonstrated to induce HSP responses, little evidence is available whether increased HSP levels provide enhanced protection against oxidative stress under elevated yet sublethal temperatures. We studied relationships between oxidative stress and HSPs in a physiological model by using Garra rufa (doctor fish), a fish species naturally acclimatized to different thermal conditions. We compared fish naturally living in a hot spring with relatively high water temperature (34.4±0.6 °C) to those living in normal river water temperature (25.4±4.7 °C), and found that levels of all the studied HSPs (HSP70, HSP60, HSP90, HSC70 and GRP75) were higher in fish living in elevated water temperature compared with normal river water temperature. In contrast, indicators of oxidative stress, including protein carbonyls and lipid hydroperoxides, were decreased in fish living in the elevated temperature, indicating that HSP levels are inversely associated with oxidative stress. The present results provide evidence that physiologically increased HSP levels provide protection against oxidative stress and enhance cytoprotection.

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Section: Discussionsupporting

confidence: 92%

Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress

et al. 2014

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“…In agreement with this, recent studies on limb regeneration in vertebrates (axolotl) demonstrated an upregulation of the RNA transcript for Hsp70 as early as 24 hours following amputation (Levesque et al 2005), confirming earlier reports describing an increased synthesis and accumulation of a 73-kDa protein (Carlone et al 1993). Other studies showed a coordinated expression of Hsp40 and Hsp70 as part of a stress response system operating after injury in zebrafish (Tawk et al 2000). A recent accumulation of literature suggests the use of sea stars as sentinel organisms for the assessment of environmental pollution.…”

Section: Discussionsupporting

confidence: 87%

Coelomocytes and post-traumatic response in the common sea star Asterias rubens

Pinsino¹,

Thorndyke²,

Matranga³

2007

Cell Stress Chaper

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Coelomocytes are recognized as the main cellular component of the echinoderm immune system. They are the first line of defense and their number and type can vary dramatically during infections or following injury. Sea stars have been used as a model system to study the regeneration process after autotomy or predation. In the present study we examined the cellular and biochemical responses of coelomocytes from the European sea star Asterias rubens to traumatic stress using immunochemical and biochemical approaches. In terms of trauma and post-traumatic stress period, here we consider the experimental arm amputation and the repair phase involved in the first 24 hours postamputation, which mimicked a natural predation event. Four cell morphotypes were distinguishable in the coelomic fluid of both control and post-traumatic-stressed animals (phagocytes, amoebocytes, vibratile cells, hemocytes), but phagocytes were the major components, accounting for about 95% of the total population. Thus, the effects measured relate to the overall population of coelomocytes. A modest increase in the total number of freely circulating coelomocytes was observed 6 hours post-amputation. Interestingly, a monoclonal antibody (McAb) to a sea urchin embryo adhesion protein (toposome) cross-reacted with isolated sea star coelomocytes and stained the coelomic epithelium of control animals with an increase in trauma-stressed arms. In addition, coelomocytes from trauma-stressed animals showed a time-dependent increase in Hsp70 levels, as detected by both immunocytochemistry and immunoblotting within 24 hours after arm tip amputation, with a peak at 6 hours after amputation. Our findings indicate a clear role for coelomocytes and classic stress molecules in the post-traumatic stress associated with the early repair phase of regeneration.

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“…These might be related to nerves, as innervation has been proven to be necessary for teleost fin regeneration (Goss and Stagg, 1957; Géraudie and Singer, 1979). They may also be factors related to injury and wounding, because the heat shock protein genes Hsp40 and Hsc70 have recently been reported to be transcribed in a large intra‐ray portion underlying the amputation plane (Tawk et al, 2000). The participation of other, as yet unknown signals cannot be ruled out.…”

Section: Discussionmentioning

confidence: 99%

Cell proliferation during blastema formation in the regenerating teleost fin

Santos-Ruíz

Santamaría

Ruiz-Sánchez

et al. 2002

Developmental Dynamics

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Epimorphic regeneration in teleost fins occurs through the establishment of a balanced growth state in which a blastema gives rise to all the mesenchymal cells, whereas definite areas of the epidermis proliferate leading to its extension, thus, allowing the enlargement of the whole structure. This type of regeneration involves specific mechanisms that temporally and spatially regulate cell proliferation. To understand how the blastema is formed and how this growth situation is set up, we investigated cell proliferation patterns in the regenerating fin of the goldfish Carassius auratus from the time of amputation to that of blastema formation by using proliferating cell nuclear antigen immunostaining and bromodeoxyuridine labeling. Wound closure and apical epidermal cap formation took place by epidermal migration and rearrangement, without the contribution of cell proliferation. As soon as the apical cap had formed, the epidermis started to proliferate at its lateral surfaces, in which all layers maintained cycling for the duration of the studied process. The distal epidermal cap, on the contrary, presented very few cycling cells, and its cytoarchitecture was indicative of continuous remodeling due to ray growth. The basal layer of this epidermal cap showed a typical morphology and remained nonproliferative whilst in contact with the proliferating blastema. Proliferation in the mesenchymal compartment of the ray started far from the amputation plane. Subsequently, cycling cells approached that location, until they formed the blastema in contact with the apical epidermal cap. Differences observed between the epidermis and mesenchyma, regarding activation of the cell cycle and the establishment of proliferative patterns, suggest that differential mechanisms regulate cell proliferation in each of these compartments during the initial stages of regeneration.

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Zebrafish Hsp40 and Hsc70 genes are both induced during caudal fin regeneration

Cited by 21 publications

References 16 publications

Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress

Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress

Coelomocytes and post-traumatic response in the common sea star Asterias rubens

Cell proliferation during blastema formation in the regenerating teleost fin

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