Zebrafish gut colonization by mCherry-labelled lactic acid bacteria

Russo, Pasquale; Iturria, Iñaki; Mohedano, María Luz; Caggianiello, Graziano; Rainieri, Sandra; Fiocco, Daniela; Pardo, Miguel Ángel; López, Paloma; Spano, Giuseppe

doi:10.1007/s00253-014-6351-x

Cited by 82 publications

(63 citation statements)

References 53 publications

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“…However, the contribution of extracellular polysaccharides in bacterial in vivo adhesion to the intestinal epithelium has not yet been validated (Ruas-Madiedo et al 2008). Recently, the in vivo colonization and persistence of exogenous LAB including L. plantarum Lp90, an EPS-producing strain, have been reported, using zebrafish larvae (Russo et al 2015). Even in this case, contrasting data exist regarding the role of EPS on bacterial adhesion passing from in vitro to in vivo models.…”

Section: Exopolysaccharides-host Interactionsmentioning

confidence: 96%

Exopolysaccharides produced by lactic acid bacteria: from health-promoting benefits to stress tolerance mechanisms

Caggianiello

Kleerebezem

Spano

2016

Appl Microbiol Biotechnol

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303

181

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A wide range of lactic acid bacteria (LAB) is able to produce capsular or extracellular polysaccharides, with various chemical compositions and properties. Polysaccharides produced by LAB alter the rheological properties of the matrix in which they are dispersed, leading to typically viscous and "ropy" products. Polysaccharides are involved in several mechanisms such as prebiosis and probiosis, tolerance to stress associated to food process, and technological properties of food. In this paper, we summarize the beneficial properties of exopolysaccharides (EPS) produced by LAB with particular attention to prebiotic properties and to the effect of exopolysaccharides on the LAB-host interaction mechanisms, such as bacterial tolerance to gastrointestinal tract conditions, ability of ESP-producing probiotics to adhere to intestinal epithelium, their immune-modulatory activity, and their role in biofilm formation. The pro-technological aspect of exopolysaccharides is discussed, focusing on advantageous applications of EPS in the food industry, i.e., yogurt and gluten-free bakery products, since it was found that these microbial biopolymers positively affect the texture of foods. Finally, the involvement of EPS in tolerance to stress conditions that are commonly encountered in fermented beverages such as wine is discussed.

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Section: Exopolysaccharides-host Interactionsmentioning

confidence: 96%

Exopolysaccharides produced by lactic acid bacteria: from health-promoting benefits to stress tolerance mechanisms

Caggianiello

Kleerebezem

Spano

2016

Appl Microbiol Biotechnol

Self Cite

303

181

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“…The role that lactococcin 972 (a bacteriocin produced by Lactococcus lactis) plays in the stabilization of gene expression in L. lactis was studied by monitoring expression of the mCherry gene (33). The adhesion of L. plantarum and Lactobacillus fermentum strains to the intestinal tract of zebrafish was studied by transferring plasmid pRCR12, containing the mCherry gene, into the bacterial cells (34). Grimm and coworkers (35) used the expression of the mCherry gene to study interactions of bifidobacteria with mammalian host cells.…”

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confidence: 99%

Use of the mCherry Fluorescent Protein To Study Intestinal Colonization by Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 in Mice

Zyl

Deane

Dicks

2015

Appl Environ Microbiol

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Lactic acid bacteria (LAB) are natural inhabitants of the gastrointestinal tract (GIT) of humans and animals, and some LAB species receive considerable attention due to their health benefits. Although many papers have been published on probiotic LAB, only a few reports have been published on the migration and colonization of the cells in the GIT. This is due mostly to the lack of efficient reporter systems. In this study, we report on the application of the fluorescent mCherry protein in the in vivo tagging of the probiotic strains Enterococcus mundtii ST4SA and Lactobacillus plantarum 423. The mCherry gene, encoding a red fluorescent protein (RFP), was integrated into a nonfunctional region on the genome of L. plantarum 423 by homologous recombination. In the case of E. mundtii ST4SA, the mCherry gene was cloned into the pGKV223D LAB/Escherichia coli expression vector. Expression of the mCherry gene did not alter the growth rate of the two strains and had no effect on bacteriocin production. Both strains colonized the cecum and colon of mice. Lactic acid bacteria (LAB) are common inhabitants of a healthy human gastrointestinal tract (GIT). Lactobacillus spp. are used as starter cultures in many fermented foods and are well known for their probiotic properties and the exclusion of pathogens from the GIT (1-6). Some strains have been closely associated with the treatment of gastrointestinal disorders, lactose intolerance, and stimulation of the immune system (7,8).Despite the increasing consumer interest in probiotic LAB, the mechanisms whereby these bacteria exert their beneficial effects in the GIT are not well understood (9). Although simulated gastrointestinal models (10-14) produced valuable data on the colonization of probiotic LAB and the exclusion of pathogens, the method remains an in vitro approach and is not a true reflection of in situ conditions. Labeling of probiotic bacteria with genes expressing fluorophores allows studying colonization and competition between gut microorganisms in vivo (15)(16)(17)(18)(19).Since the discovery of the green fluorescent protein (GFP) by Shimomura et al. (20), a number of genetic variants that emit light at longer wavelengths, and that are more suited for in vivo animal studies, have been described (21-23). The mCherry red fluorescence protein (RFP), a variant of the Discosoma red (DsRed) protein (24,25), is excited at wavelengths longer than 600 nm and is photostable (26-28). Studies conducted with Bacillus subtilis (29) and Escherichia coli (30) showed that expression of the mCherry protein, even at high levels, had no effect on the cell's physiology. Tauer and coworkers (31) used the mCherry gene to study recombinant protein expression in Lactobacillus plantarum. Mohedano and coworkers (32) adapted the broad-host-range vector pNZ8048 to study gene expression in Lactobacillus and showed that the mCherry construct could be used to study complex promoter induction mechanisms, such as bacteriocin production by Lactobacillus acidophilus. The role that lactococcin 972 (...

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“…The ability of an invader to adhere and clolonise the native communities has a pivotal role (Russo et al, 2015), but the true drivers of the success of invasion seem to be related to the characteristics of the resident community, such as diversity and evenness, competition for resources, as well as changes in environmental conditions (van Elsas et al, 2012;Iasur-Kruh et al, 2011;Burmølle et al, 2006;Guillier et al, 2008;Mejlholm and Dalgaard, 2007;Zhao et al, 2006). A negative correlation between soil microbial diversity and the survival of Escherichia coli O157:H7 was reported by van Elsas et al (2012).…”

Section: Introductionmentioning

confidence: 99%