Zebrafish fin and heart: what's special about regeneration?

Sehring, Ivonne Margarete; Jahn, Christopher; Weidinger, Gilbert

doi:10.1016/j.gde.2016.05.011

Cited by 60 publications

(57 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After injury, the production of ROS increases as a consequence of the inflammatory response, and this increase in ROS production modifies the redox status, affecting the process of regeneration by diminishing stem/progenitor cell self-renewal as well as the presence of active stemness factors dictating cell differentiation (Kobayashi and Suda, 2012). While our system supports that a decrease in ROS is necessary to induce proliferation of retinal stem/progenitors and subsequent regeneration, it is worth noting that it has also been reported that an initial increase in ROS is necessary for regeneration in some species (Sehring et al, 2016; Serras, 2016). This apparent contradiction will have to be resolved through future experiments but it is possible that an initial increase in ROS is necessary but without the activation of signaling pathways, the redox status is not changed, and regeneration will not ensue.…”

Section: Discussionsupporting

confidence: 71%

A biochemical basis for induction of retina regeneration by antioxidants

Echeverri-Ruíz

Haynes

Landers

et al. 2018

Developmental Biology

View full text Add to dashboard Cite

The use of antioxidants in tissue regeneration has been studied, but their mechanism of action is not well understood. Here, we analyze the role of the antioxidant N-acetylcysteine (NAC) in retina regeneration. Embryonic chicks are able to regenerate their retina after its complete removal from retinal stem/progenitor cells present in the ciliary margin (CM) of the eye only if a source of exogenous factors, such as FGF2, is present. This study shows that NAC modifies the redox status of the CM, initiates self-renewal of the stem/progenitor cells, and induces regeneration in the absence of FGF2. NAC works as an antioxidant by scavenging free radicals either independently or through the synthesis of glutathione (GSH), and/or by reducing oxidized proteins through a thiol disulfide exchange activity. We dissected the mechanism used by NAC to induce regeneration through the use of inhibitors of GSH synthesis and the use of other antioxidants with different biochemical structures and modes of action, and found that NAC induces regeneration through its thiol disulfide exchange activity. Thus, our results provide, for the first time, a biochemical basis for induction of retina regeneration. Furthermore, NAC induction was independent of FGF receptor signaling, but dependent on the MAPK (pErk1/2) pathway.

show abstract

Section: Discussionsupporting

confidence: 71%

A biochemical basis for induction of retina regeneration by antioxidants

Echeverri-Ruíz

Haynes

Landers

et al. 2018

Developmental Biology

View full text Add to dashboard Cite

show abstract

“…These animals can survive substantial heart and limb injuries, and efficiently replace the lost parts with new functional tissues12345678910. During reconstruction of the missing part, remnant injury-associated cells reactivate morphogenetic programs, proliferate and recreate a replicate of the original structure.…”

mentioning

confidence: 99%

The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin

Pfefferli

Jaźwińska

2017

Nat Commun

View full text Add to dashboard Cite

The existence of common mechanisms regulating organ regeneration is an intriguing concept. Here we report on a regulatory element that is transiently activated during heart and fin regeneration in zebrafish. This element contains a ctgfa upstream sequence, called careg, which is induced by TGFβ/Activin-β signalling in the peri-injury zone of the myocardium and the fin mesenchyme. In addition, this reporter demarcates a primordial cardiac layer and intraray osteoblasts. Using genetic fate mapping, we show the regenerative competence of careg-expressing cells. The analysis of the heart reveals that the primordial cardiac layer is incompletely restored after cryoinjury, whereas trabecular and cortical cardiomyocytes contribute to myocardial regrowth. In regenerating fins, the activated mesenchyme of the stump gives rise to the blastema. Our findings provide evidence of a common regenerative programme in cardiomyocytes and mesenchyme that opens the possibility to further explore conserved mechanisms of the cellular plasticity in diverse vertebrate organs.

show abstract

“…Potential migration attractants during healing are Igf (Chablais & Jaźwińska, ) and ROS (Sehring et al. ), which regulate wound closure and are expressed at wound epidermis (Gauron et al. ).…”

Section: Discussionmentioning

confidence: 99%

“…10E). Potential migration attractants during healing are Igf (Chablais & Ja zwi nska, 2010) and ROS (Sehring et al 2016), which regulate wound closure and are expressed at wound epidermis (Gauron et al 2013). actba (Ja zwi nska et al 2007) or retinoic acid (Blum & Begemann, 2015) signalling is also involved in maintenance and/or de-differentiation of distally migrating fibroblasts or osteoblasts.…”

Section: During Wound Healing Width Is Established By a Bioelectricimentioning

confidence: 99%

Widening control of fin inter‐rays in zebrafish and inferences about actinopterygian fins

et al. 2018

View full text Add to dashboard Cite

The amputation of a teleost fin rapidly triggers an intricate maze of hierarchically regulated signalling processes which ultimately reconstruct the diverse tissues of the appendage. Whereas the generation of the fin pattern along the proximodistal axis brings with it several well-known developmental regulators, the mechanisms by which the fin widens along its dorsoventral axis remain poorly understood. Utilizing the zebrafish as an experimental model of fin regeneration and studying more than 1000 actinopterygian species, we hypothesized a connection between specific inter-ray regulatory mechanisms and the morphological variability of inter-ray membranes found in nature. To tackle these issues, both cellular and molecular approaches have been adopted and our results suggest the existence of two distinguishable inter-ray areas in the zebrafish caudal fin, a marginal and a central region. The present work associates the activity of the cell membrane potassium channel kcnk5b, the fibroblast growth factor receptor 1 and the sonic hedgehog pathway to the control of several cell functions involved in inter-ray wound healing or dorsoventral regeneration of the zebrafish caudal fin. This ray-dependent regulation controls cell migration, cell-type patterning and gene expression. The possibility that modifications of these mechanisms are responsible for phenotypic variations found in euteleostean species, is discussed.

show abstract

Zebrafish fin and heart: what's special about regeneration?

Cited by 60 publications

References 85 publications

A biochemical basis for induction of retina regeneration by antioxidants

A biochemical basis for induction of retina regeneration by antioxidants

The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin

Widening control of fin inter‐rays in zebrafish and inferences about actinopterygian fins

Contact Info

Product

Resources

About