Zebrafish elav/HuC homologue as a very early neuronal marker

Kim, Cheol‐Hee; Ueshima, Emiko; Muraoka, Osamu; Tanaka, Hidekazu; Yeo, Sang-Yeob; Huh, Tae‐Lin; Miki, Naomasa

doi:10.1016/0304-3940(96)13021-4

Cited by 274 publications

(115 citation statements)

References 16 publications

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“…3D-3F). However, scrt-expressing cells completely overlapped with huC/elavl3-positive differentiating neurons in the brain as well as cranial ganglia, indicating that scrt is expressed in most differentiating neurons (3G-I) (Kim et al, 1996). These results suggest that the expression of zebrafish scrt genes is specifically restricted to differentiating neurons during early neurogenesis.…”

Section: Scratch Genes Are Expressed In Differentiating Neuronsmentioning

confidence: 81%

“…Anti-sense RNA probes were transcribed in vitro using T7 RNA polymerase and digoxigenin or fluorescein-labeled UTP. Antisense digoxigeninlabeled RNA probes for ngn1 (Kim et al, 1997) and huC/elavl3 (Kim et al, 1996) were produced using a digoxigenin-RNA labeling Kit (Roche, Germany) according to the manufacturer's instructions. Whole-mount in situ hybridization was performed as previously described (Gwak et al, 2010).…”

Section: Whole-mount In Situ Hybridizationmentioning

confidence: 99%

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Neuron-Specific Expression of Scratch Genes during Early Zebrafish Development

Dam

Kim

Moon

et al. 2011

Molecules and Cells

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Section: Scratch Genes Are Expressed In Differentiating Neuronsmentioning

confidence: 81%

Section: Whole-mount In Situ Hybridizationmentioning

confidence: 99%

Neuron-Specific Expression of Scratch Genes during Early Zebrafish Development

Dam

Kim

Moon

et al. 2011

Molecules and Cells

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“…By 48 hpf, ath5 is expressed in retinoblast cells and, possibly, postmitotic neurons before full differentiation but not in differentiated cells in the central retina. In contrast, the zebrafish elavl3 gene encodes an RNA-binding protein homologous to Drosophila elav that serves as an early marker for differentiated neurons (30). In vertebrate retinae, elavl3 is expressed in ganglion cells and the majority of amacrine cells in the inner nuclear layer (31).…”

Section: Results M850 Mutant Embryos Display Reduction In Cell Numbermentioning

confidence: 99%

Depletion of minichromosome maintenance protein 5 in the zebrafish retina causes cell-cycle defect and apoptosis

Ryu

Holzschuh

Erhardt

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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In multicellular organisms, the control of genome duplication and cell division must be tightly coordinated. Essential roles of the minichromosome maintenance (MCM) proteins for genome duplication have been well established. However, no genetic model has been available to address the function of MCM proteins in the context of vertebrate organogenesis. Here, we present positional cloning of a zebrafish mcm5 mutation and characterization of its retina phenotype. In the retina, mcm5 expression correlates closely with the pattern of cell proliferation. By the third day of development, mcm5 is down-regulated in differentiated cells but is maintained in regions containing retinal stem cells. We demonstrate that a gradual depletion of maternally derived MCM5 protein leads to a prolonged S phase, cell-cycle-exit failure, apoptosis, and reduction in cell number in mcm5 m850 mutant embryos. Interestingly, by the third day of development, increased apoptosis is detectable only in the retina, tectum, and hindbrain but not in other late-proliferating tissues, suggesting that different tissues may employ distinct cellular programs in responding to the depletion of MCM5.stem cell ͉ cell proliferation ͉ ciliary marginal zone ͉ embryogenesis ͉ development P roper duplication of the genome during the cell cycle is of paramount importance for the life of an organism. In eukaryotes, each replication origin is ''licensed'' to fire once per cell cycle through the cell-cycle-dependent formation and destruction of a prereplication complex (preRC) (1-3). A key component of this preRC is a family of six structurally related proteins, MCM2 through -7, which are evolutionarily conserved in all eukaryotes. The MCM proteins were originally identified as proteins required for minichromosome maintenance in Saccharomyces cerevisiae (4). MCM2 through -7 belong to a distinct subgroup of the large AAAϩ ATPase family (5) and share a conserved central region of Ϸ200 amino acids (MCM box). Biochemical studies in Xenopus have established the role of MCM2 through -7 as replication-licensing factors (6-8). Subsequent studies illustrate that proper orchestration of the functional interactions among MCM2 through -7 proteins and other components of the preRC by cell-cycledependent protein kinases results in initiation of DNA synthesis once every cell cycle (9, 10). The MCM2 through -7 proteins appear to form heterohexamers and play important roles in initiation and elongation during DNA replication (4,11,12). Furthermore, recent evidence supports involvement of MCMs in many other chromosome transactions, including transcription, chromatin remodeling, and genome stability (13). However, in vivo analysis of MCMprotein functions in multicellular organism has been scarce. Here, we report the isolation, positional cloning, and in-depth characterization of a vertebrate mutant in an MCM-family protein. We demonstrate that the zebrafish m850 allele harbors a mutation in the mcm5 gene and exhibits developmental defects in lateproliferating tissues, including the re...

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“…Whole Mount in Situ Hybridization-Details of the RNA in situ hybridizations with zebrafish embryos were described for the various probes: huC (22), flt4 (23), and ephrinB2a (23). To detect the ␤-galactosidase co-injected with the synthesized mRNAs, the embryos were fixed in 4% paraformaldehyde in phosphate-buffered saline at 4°C for 1 h and stained in 5-bromo-4-chloro-3-indolyl-␤-D-galactopyranoside (X-gal) staining buffer.…”

Section: Cloning Of the Mouse Mib2 Gene And Generation Of Mutations Imentioning

confidence: 99%

Mind Bomb-2 Is an E3 Ligase for Notch Ligand

Koo

Yoon

Yoo

et al. 2005

Journal of Biological Chemistry

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105

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The zebrafish gene, mind bomb (mib), encodes a protein that positively regulates of the Delta-mediated Notch signaling. It interacts with the intracellular domain of Delta to promote its ubiquitination and endocytosis. In our search for the mouse homologue of zebrafish mind bomb, we cloned two homologues in the mouse genome: a mouse orthologue (mouse mib1) and a paralogue, named mind bomb-2 (mib2), which is evolutionarily conserved from Drosophila to human. Both Mib1 and Mib2 have an E3 ubiquitin ligase activity in their C-terminal RING domain and interact with Xenopus Delta (XD) via their N-terminal region. Mib2 is also able to ligate ubiquitin to XD and shift the membrane localization of Delta to intracellular vesicles. Importantly, Mib2 rescues both the neuronal and vascular defects in the zebrafish mib ta52b mutants. In contrast to the functional similarities between Mib1 and Mib2, mib2 is highly expressed in adult tissues, but almost not at all in embryos, whereas mib1 is abundantly expressed in both embryos and adult tissues. These data suggest that Mib2 has functional similarities to Mib1, but might have distinct roles in Notch signaling as an E3 ubiquitin ligase.

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Zebrafish elav/HuC homologue as a very early neuronal marker

Cited by 274 publications

References 16 publications

Neuron-Specific Expression of Scratch Genes during Early Zebrafish Development

Neuron-Specific Expression of Scratch Genes during Early Zebrafish Development

Depletion of minichromosome maintenance protein 5 in the zebrafish retina causes cell-cycle defect and apoptosis

Mind Bomb-2 Is an E3 Ligase for Notch Ligand

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