Zebrafish eggs used as bioreactors for the production of bioactive tilapia insulin-like growth factors

Hu, Shao‐Yang; Liao, Chia-Hsuan; Lin, Yi‐Pei; Li, Yen-Hsing; Gong, Hong‐Yi; Lin, Gen-Hwa; Kikuchi, Kôichi; Yang, Ting‐Hua; Wu, Jen‐Leih

doi:10.1007/s11248-010-9388-3

Cited by 13 publications

(6 citation statements)

References 37 publications

(39 reference statements)

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“…Additionally, owing to their small size, rapid development, and short life cycle, zebrafish are often used in gene expression studies. More importantly, the zebrafish are transparent and easy to monitor by real-time images (Zon & Peterson, 2005 ; Hu et al, 2011 ). As shown in Figure 5(D) , the expression of green fluorescent protein was observed in zebrafish brain after being treated with PT-02/pIRES2-EGFP complexes.…”

Section: Resultsmentioning

confidence: 99%

Transferrin receptor targeting segment T7 containing peptide gene delivery vectors for efficient transfection of brain tumor cells

Kang¹,

Zeng²,

Tian

et al. 2022

Drug Delivery

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Successful gene therapy for brain tumors are often limited by two important factors, the existence of blood brain barrier (BBB) and inefficient transfection of brain tumor cells. In this study, we designed a series of peptide-based gene delivery vectors decorated with T7 segment for binding the transferrin (Tf) receptors which were highly expressed on brain tumor cells, and evaluated their ability of gene delivery. The physicochemical properties of peptide vectors or peptide/DNA complexes were studied as well. The in vitro transfection efficiency was investigated in normal and glioma cell lines. Among these complexes, PT-02/DNA complexes showed the highest transfection efficiency in glioma cells and low cytotoxicity in normal cell lines, and it could transport DNA across the BBB model in vitro . Furthermore, PT-02/DNA could deliver pIRES2-EGFP into the brain site of zebrafish in vivo . The designed peptide vectors offered a promising way for glioma gene therapy.

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Section: Resultsmentioning

confidence: 99%

Transferrin receptor targeting segment T7 containing peptide gene delivery vectors for efficient transfection of brain tumor cells

Kang¹,

Zeng²,

Tian

et al. 2022

Drug Delivery

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“…Biologically active tilapia insulin‐like growth factors were successfully produced in transgenic zebrafish (Hu et al . 2011).…”

Section: Genetically Modified Fish Applicationsmentioning

confidence: 99%

“…Because there are 17 different amino acids between Nile tilapia (Oreochromis niloticus) and human insulin, only humanized tilapia insulin may be used for the treatment of human diabetes (Wright et al 2014). Biologically active tilapia insulin-like growth factors were successfully produced in transgenic zebrafish (Hu et al 2011).…”

Section: Bioreactor Systemsmentioning

confidence: 99%

A comprehensive review on genetically modified fish: key techniques, applications and future prospects

Wang

Hamid

Jia

et al. 2021

Reviews in Aquaculture

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Genetically modified fish is a general term used for whole fish whose DNA has been artificially altered by directly integrating (or deleting) single or multiple genes to introduce or modify a targeted character. Different techniques have been performed to produce genetically modified fish. Importantly, the introduction of various types of fluorescent proteins has dramatically expanded the range of applications of genetically modified fish. Currently, genetically modified fish have been widely employed as experimental models for medical science, pharmacology and environmental toxicology. The commercial application of genetically modified fish in aquaculture is still up for debate, although the first commercialization of genetically modified fish, GloFish, was in 2003, and further, the United States and Canada approved the commercial production and sale of the AquAdvantage Salmon. In this review, we discuss the principles and developmental history of fish transgenesis via gene transfer and gene-editing techniques, and their applications in diverse fields. Further, challenges and prospects associated with the genetically modified fish are discussed.

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“…Transgenic fish can also be used as a bioreactor. Several studies have used zebrafish eggs to produce recombinant proteins such as human coagulation factor VII [68], luteinizing hormone [69] and insulin-like growth factors [70].…”

Section: Transgenic Breedingmentioning

confidence: 99%

Fish genome manipulation and directional breeding

Zhu

Sun

2015

Sci. China Life Sci.

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Aquaculture is one of the fastest developing agricultural industries worldwide. One of the most important factors for sustainable aquaculture is the development of high performing culture strains. Genome manipulation offers a powerful method to achieve rapid and directional breeding in fish. We review the history of fish breeding methods based on classical genome manipulation, including polyploidy breeding and nuclear transfer. Then, we discuss the advances and applications of fish directional breeding based on transgenic technology and recently developed genome editing technologies. These methods offer increased efficiency, precision and predictability in genetic improvement over traditional methods. fish directional breeding, polyploidy breeding, nuclear transfer, transgenic fish, genome editing Citation:Ye D, Zhu ZY, Sun YH. Fish genome manipulation and directional breeding. Sci China Life Sci, 2015Sci, , 58: 170 -177, doi: 10.1007 Fish are one of the most important sources of protein for humans. As a result of declines in wild fisheries, aquaculture has become one of the fastest developing agricultural industries worldwide [1]. To increase the sustainability of aquaculture, culturists commonly conduct selective breeding to develop strains that perform well in captivity. Unfortunately, the sustainability of many sectors of the industry is negatively affected by inbreeding depression, disease outbreaks, under production, and low meat quality [2]. To address these issues, there is an urgent need for the development of high quality fish strains that have high growth rates, disease-resistance, and/or higher nutritional value.Traditional crossbreeding methods such as intra-species crossbreeding [3] and inter-species hybridization [4] have been successfully used for several decades. However, crossbreeding requires multiple-generations of hybridization to introduce a desirable trait to a given strain. Additionally, the outcomes of these methods are unpredictable because the underlying mechanisms controlling desirable traits are unknown. Thus, there is a need to develop more efficient, precise and predictable techniques for producing production scale numbers of high-quality fish. We review the history of fish breeding methods based on classical genome manipulation approaches, including polyploidy breeding [5] and nuclear transfer [6]. Then, we discuss directional breeding of fish based on transgenic technology and recently developed genome editing technologies. Advances in breeding methodology based on classical genome manipulation and recently developed genome editing methods will likely play a major role in the future of genetic breeding in fish.

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Zebrafish eggs used as bioreactors for the production of bioactive tilapia insulin-like growth factors

Cited by 13 publications

References 37 publications

Transferrin receptor targeting segment T7 containing peptide gene delivery vectors for efficient transfection of brain tumor cells

Transferrin receptor targeting segment T7 containing peptide gene delivery vectors for efficient transfection of brain tumor cells

A comprehensive review on genetically modified fish: key techniques, applications and future prospects

Fish genome manipulation and directional breeding

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