Zebrafish dives into food research: effectiveness assessment of bioactive compounds

Caro, Mercedes; Iturria, Iñaki; Martinez-Santos, M.; Pardo, Miguel Ángel; Rainieri, Sandra; Tueros, Itziar; Navarro, Virginia

doi:10.1039/c6fo00046k

Cited by 16 publications

(10 citation statements)

References 77 publications

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“…Nevertheless, they could be unsuited for certain types of studies [14]. In the past decade, teleost species have been regarded as excellent alternative models for studying human diseases [15,16] and now constitute an emerging method for assessing bioactive compounds in food research [17]. A number of in vitro and in vivo studies have highlighted the applicability of several fish species within the areas of lipid metabolism and adipose tissue biology [18–23].…”

Section: Introductionmentioning

confidence: 99%

Caffeic acid and hydroxytyrosol have anti-obesogenic properties in zebrafish and rainbow trout models

et al. 2017

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Some natural products, known sources of bioactive compounds with a wide range of properties, may have therapeutic values in human health and diseases, as well as agronomic applications. The effect of three compounds of plant origin with well-known dietary antioxidant properties, astaxanthin (ATX), caffeic acid (CA) and hydroxytyrosol (HT), on zebrafish (Danio rerio) larval adiposity and rainbow trout (Onchorynchus mykiss) adipocytes was assessed. The zebrafish obesogenic test (ZOT) demonstrated the anti-obesogenic activity of CA and HT. These compounds were able to counteract the obesogenic effect produced by the peroxisome proliferator-activated receptor gamma (PPARγ) agonist, rosiglitazone (RGZ). CA and HT suppressed RGZ-increased PPARγ protein expression and lipid accumulation in primary-cultured rainbow trout adipocytes. HT also significantly reduced plasma triacylglycerol concentrations, as well as mRNA levels of the fasn adipogenic gene in the adipose tissue of HT-injected rainbow trout. In conclusion, in vitro and in vivo approaches demonstrated the anti-obesogenic potential of CA and HT on teleost fish models that may be relevant for studying their molecular mode of action. Further studies are required to evaluate the effect of these bioactive components as food supplements for modulating adiposity in farmed fish.

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Section: Introductionmentioning

confidence: 99%

Caffeic acid and hydroxytyrosol have anti-obesogenic properties in zebrafish and rainbow trout models

et al. 2017

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“…Zebrafish has similar physiology and anatomy to mammals as well as being present orthologous genes for 70% of the human ones. However, the central reasons for the wide use of Zebrafish in research area are the low cost for maintenance and experimentation, their high fecund and rapid breeding, and the feasibility of the in vivo visualization of complex responses (Caro et al, 2016).…”

Section: Zebrafish Modelmentioning

confidence: 99%

“…The antioxidants activity of bioactives in zebrafish model can be determined through the direct measurement of reactive oxygen species production and biomarkers produced by oxidative damage under both normal physiological conditions and after treatment with an oxidative stressor (Caro et al, 2016). Among those approaches in zebrafish model, the primary method to investigate the antioxidant activity of bioactives is to detect the quantity of ROS using fluorescence indicators.…”

Section: Zebrafish Modelmentioning

confidence: 99%

“…For that, fluorescent probes such as DCFH-DA (dichlorodihydrofluorescein diacetate) and HPF (hydroxyphenyl fluorescein) are utilized in which these fluorescent probes emit a fluorescence signal once they are oxidized by ROS. Thus, this method enables rapid and direct detection of oxidative stress (Caro et al, 2016). For instance, Yang et al (2012) used DCFH-DA to detect the generation of ROS in zebrafish upon ultraviolet (UV) B radiation and to investigate the antioxidant effect of quercitrin.…”

Section: Zebrafish Modelmentioning

confidence: 99%

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Should the in vitro colorimetric assays in antioxidant and lipid oxidation evaluation be abandoned?: A critical review focusing on bioactive molecule screening assays in in vitro and in vivo models

Shahidi¹,

Yeo²

2020

JFB

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Increasing evidence has proven the potent antioxidative effectiveness of bioactives in natural products for preventing/suppressing chronic diseases. In this connection, the development of efficient methods that are suitable to screen bioactives in vitro and in vivo tests has taken place. Thus, a variety of assays have been used in the extraction of bioactives, testing of their antioxidant potential in both in vitro and in vivo model, and evaluating lipid oxidation are comprehensively discussed here. This review mainly focuses on the principle and the use of individual assays in both in vitro and in vivo models. Given that induvial assays have pros and cons due to the discrepancy in the reaction environment and applied biological system, application to the various assays in order to complement the drawbacks of each assay is highly recommended to obtain the reasonable information from experimental trials.

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“…The zebrafish has many advantages that provide a useful framework to comparatively investigate chemical bioactivity in vivo (reviewed by: Bugel et al , 2014; Caro et al , 2016). The zebrafish genome also shares 70% homology with the human genome, which is valuable for translational medicine, drug discovery, and large-scale chemical screens (Howe et al , 2013).…”

Section: Introductionmentioning

confidence: 99%

Multidimensional chemobehavior analysis of flavonoids and neuroactive compounds in zebrafish

Bugel

Tanguay

2018

Toxicology and Applied Pharmacology

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The comparative analysis of complex behavioral phenotypes is valuable as a reductionist tool for both drug discovery and defining chemical bioactivity. Flavonoids are a diverse class of chemicals that elicit robust neuroactive and hormonal actions, though bioactivity information is limited for many, particularly for neurobehavioral endpoints. Here, we used a zebrafish larval chemomotor response (LCR) bioassay to comparatively evaluate a suite of 24 flavonoids, and in addition a panel of 30 model neuroactive compounds representing diverse modes of action (e.g. caffeine, chlorpyrifos, methamphetamine, nicotine, picrotoxin). Naïve larval zebrafish were exposed to concentration ranges of each compound at 120 hour post-fertilization (hpf) and locomotor activity measured for 5 h. The model neuroactive compounds were largely behaviorally bioactive (20 of 30) with most effects phenotypic of their known modes of action. Flavonoids rapidly and broadly elicited hyperactive locomotor effects (22 of 24). Multidimensional analyses compared responses over time and identified three distinct bioactive groups of flavonoids based on efficacy and potency. Using GABAergics to modulate hyperactive responses, two flavonoids, (S)-equol and kaempferol were tested for GABA receptor antagonism, as well as a known GABA receptor antagonist, picrotoxin. Pharmacological intervention with positive allosteric modulators of the GABA receptor, alfaxalone and chlormethiazole, ameliorated the hyperactive response to picrotoxin, but not for (S)-equol or kaempferol. Taken together, these studies demonstrate that flavonoids are differentially bioactive and that the chemobehavioral effects likely do not involve a GABA receptor mediated mode of action. Overall, the integrative zebrafish platform provides a useful framework for comparatively evaluating high-content chemobehavioral data for sets of structurally- and mechanistically-related flavonoids and neuroactive compounds.

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Zebrafish dives into food research: effectiveness assessment of bioactive compounds

Cited by 16 publications

References 77 publications

Caffeic acid and hydroxytyrosol have anti-obesogenic properties in zebrafish and rainbow trout models

Caffeic acid and hydroxytyrosol have anti-obesogenic properties in zebrafish and rainbow trout models

Should the in vitro colorimetric assays in antioxidant and lipid oxidation evaluation be abandoned?: A critical review focusing on bioactive molecule screening assays in in vitro and in vivo models

Multidimensional chemobehavior analysis of flavonoids and neuroactive compounds in zebrafish

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