Zebrafish Development: High‐Throughput Test Systems to Assess Developmental Toxicity

Padilla, Stephanie

doi:10.1002/9781118538203.ch20

Cited by 2 publications

(1 citation statement)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…That is assuming, however, that the internal concentration in the zebrafish larva equates with the nominal concentration. Because these chemicals are, in general, highly lipophilic (Table 1), it likely that the internal concentration of these chemicals in the zebrafish is orders of magnitude higher than the nominal concentration (Dishaw et al, 2014a; Padilla, 2013; Petersen and Kristensen, 1998), thereby decreasing the applicability to the human condition. Another confounder is that exposure to these chemicals in the real world does not occur in isolation; the real world exposures are most likely via multiple flame retardants (Stapleton et al, 2009), and no information exists on the toxicity of these chemicals using mixture dosing scenarios, although the zebrafish model would be an excellent one with which to assess the combinatorial toxicology of these chemicals.…”

Section: Discussionmentioning

confidence: 99%

Acute and developmental behavioral effects of flame retardants and related chemicals in zebrafish

Jarema

Hunter

Shaffer

et al. 2015

Neurotoxicology and Teratology

Self Cite

145

View full text Add to dashboard Cite

As polybrominated diphenyl ethers are phased out, numerous compounds are emerging as potential replacement flame retardants for use in consumer and electronic products. Little is known, however, about the neurobehavioral toxicity of these replacements. This study evaluated the neurobehavioral effects of acute or developmental exposure to t-butylphenyl diphenyl phosphate (BPDP), 2-ethylhexyl diphenyl phosphate (EHDP), isodecyl diphenyl phosphate (IDDP), isopropylated phenyl phosphate (IPP), tricresyl phosphate (TMPP; also abbreviated TCP), triphenyl phosphate (TPHP; also abbreviated TPP), tetrabromobisphenol A (TBBPA), tris (2-chloroethyl) phosphate (TCEP), tris (1,3-dichloroisopropyl) phosphate (TDCIPP; also abbreviated TDCPP), tri-o-cresyl phosphate (TOCP), and 2,2-,4,4′-tetrabromodiphenyl ether (BDE-47) in zebrafish (Danio rerio) larvae. Larvae (n ≈ 24 per dose per compound) were exposed to test compounds (0.4–120 µM) at sub-teratogenic concentrations either developmentally or acutely, and locomotor activity was assessed at 6 days post fertilization. When given developmentally, all chemicals except BPDP, IDDP and TBBPA produced behavioral effects. When given acutely, all chemicals produced behavioral effects, with TPHP, TBBPA, EHDP, IPP, and BPDP eliciting the most effects at the most concentrations. The results indicate that these replacement flame retardants may have developmental or pharmacological effects on the vertebrate nervous system.

show abstract